Method Development

MS-based methods play an important role in metabolomics for detecting and quantifying metabolites and metabolic flux. Our previous results lie predominantly in the following areas: 1) expanding the detectable metabolite coverage, 2) improving metabolite quantitation combining both NMR and MS, and 3) developing novel metabolic flux analysis approaches. Currently, metabolic measurement of over 1,000 small molecules (MW<2,000 Da) is now available in my Lab on a routine basis. We have developed advanced targeted and global metabolite profiling methods, for both metabolite level measurements and metabolic flux analysis.

a) H. Gu, H. Chen, Z. Pan, A. U. Jackson, N. Talaty, B. Xi, C. Kissinger, C. Duda, D. Mann, D. Raftery*, R. G. Cooks*, “Monitoring Diet Effects from Biofluids and Their Implications for Metabolomics Studies”, Anal. Chem., 79, 89-97, 2007. PMID: 17194125

b) F. Tayyari, A. N. Gowda, H. Gu, D. Raftery*, “15N-Cholamine—A Smart Isotope Tag for Combining NMR- and MS-Based Metabolite Profiling”, Anal. Chem., 85, 8715-8721, 2013. PMCID: PMC3803152

c) H. Gu*, P. Zhang, J. Zhang, D. Raftery*, “Globally Optimized Targeted Mass Spectrometry: Reliable Metabolomics Analysis with Broad Coverage”, Anal. Chem., 87, 12355-12362, 2015. PMID: 26579731

d) H. Gu, P. Carroll, J. Du, J. Zhu, F. Carnevale Neto, R. Eisenman, and D. Raftery*, “Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine”, Angew. Chem. Int. Ed., 55, 15646-15650, 2016. PMID: 2786010