Method Development

Our lab uses mass spectrometry (MS) to detect and measure small molecules involved in metabolism. We've focused on:

1. Detecting More Metabolites: Expanding the range of metabolites we can identify.

2. Better Measurements: Combining MS with other methods to improve how accurately we measure these molecules.

3. New Techniques: Creating new methods to study how these molecules move and change in the body.

Right now, we can routinely measure over 1,000 metabolites in our lab. We've developed advanced methods to profile these molecules and study their movement and changes in detail. Routinely measuring metabolites accurately is crucial for the early detection and diagnosis of diseases, creating more effective drugs and treatments to understand the underlying mechanisms of diseases. 

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b) F. Tayyari, A. N. Gowda, H. Gu, D. Raftery*, “15N-Cholamine—A Smart Isotope Tag for Combining NMR- and MS-Based Metabolite Profiling”, Anal. Chem., 85, 8715-8721, 2013. PMCID: PMC3803152

c) H. Gu*, P. Zhang, J. Zhang, D. Raftery*, “Globally Optimized Targeted Mass Spectrometry: Reliable Metabolomics Analysis with Broad Coverage”, Anal. Chem., 87, 12355-12362, 2015. PMID: 26579731

d) H. Gu, P. Carroll, J. Du, J. Zhu, F. Carnevale Neto, R. Eisenman, and D. Raftery*, “Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine”, Angew. Chem. Int. Ed., 55, 15646-15650, 2016. PMID: 2786010