Flux Analysis

The whole metabolome can be studied as a big map, in which metabolites (cities) and metabolic pathways (roads) comprise the metabolic reaction network. Metabolite levels provide “snapshots” of biochemical abundances; however, cellular metabolism in a living system is not static. In contrast, metabolic flux analysis (MFA) is able to provide a dynamic description of the cellular metabolome in a living system. Therefore, pathway-specific information from MFA is often required to fully understand a biological process. 

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2. H. Gu, J. Du, P. A. Carroll, J. Zhu, F. Carnevale Neto, R. N. Eisenman, D. Raftery*, “Quantitative Method to Investigate the Balance between Metabolism and Proteome Biomass: Starting from Glycine”, Angew. Chem. Int. Ed., 55, 15656-15650, 2016. PMID: 27860107