Protocols
RNA extraction: refer to protocols from Usha's thesis file below
Antibody purification from serum: refer to protocols from Mansi's thesis file below
a) Coupling of antigen to the column
b) Ammonium sulfate precipitation of IgG: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC380730/pdf/applmicro00053-0040.pdf
c) Antibody usage: refer to protocols from Mansi and Saurabh's thesis files below
(i) Immuno-fluorescence (IF) (ii) Immuno-precipitation (IP)
d) Affinity purification of proteins: refer to protocols from Mansi, Usha and Saurabh's thesis files below
i) GSH purification ii) Ni-NTA purification iii) GBP protocol: