Protocols

RNA extraction: refer to protocols from Usha's thesis file below

Antibody purification from serum: refer to protocols from Mansi's thesis file below

a) Coupling of antigen to the column

b) Ammonium sulfate precipitation of IgG: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC380730/pdf/applmicro00053-0040.pdf

c) Antibody usage: refer to protocols from Mansi and Saurabh's thesis files below

(i) Immuno-fluorescence (IF) (ii) Immuno-precipitation (IP)

d) Affinity purification of proteins: refer to protocols from Mansi, Usha and Saurabh's thesis files below

i) GSH purification ii) Ni-NTA purification iii) GBP protocol: