Overview of Status of Analysis, Design, Fabrication, Tests, etc.
Finalized initial design of the automation system
Began making parts and testing the water flow method with breads for risk reduction
Accomplishments from Previous Week
[Jing, Woo Jae] Discussed the layout of the complete system, research and documented necessary components
[Jing] Propose the overall design of the system
[Jing] Begin CADing the system--container layout and funnel
[Everyone] Prepare design proposal presentation
[Gregory] Background (for Report)
[Gregory] Deliverables (for Report)
[Jasmine] Title page (for Report)
[Jasmine] Appendix with Individual Component Reports (for Report)
[Gregory] Review of Existing Solutions (for Report)
[Gregory] Statement of Requirements (for Report)
Goals for Next Week (list names after each item). Use specific and measurable objectives.
[Gregory] Impact on Society (for Report)
[Gregory] Professional Responsibility (for Report)
[Jing] Continue CAD of the flow system
[Jing] Design and begin manufacture funnel
[Jasmine] Buy parts/materials for first prototype
[Gregory] Design revolving door to separate and scan the pupae
[Jasmine] Find a cheap low flow rate pump
[Woo Jae] Work on the software and hardware for color detection
Sponsor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
1/10/2020 in person
News:
MIT Mechanical Engineering grad student is working on another project for the mosquito lab, something about injection. We can get help and review from him.
Genetically modified mosquitoes will be arriving to our sponsor's lab next week (1/13 - 1/17)
member of sponsor's team (also attended this in person meeting) is available to meet any time on Tuesdays, Wednesdays, and Fridays. We just need to notify in advance
mosquito behavior/characteristics to consider in our design:
florescent green coloration will be expressed on the eyes of the mosquito pupae and larvae
genetically modified mosquitoes come in two strains: one has green florescent coloration (GFP), the other has red/crimson florescent coloration (CFP)
pupae tend to stay at the top of the water because they only need air
larvae swim up for air and down for food
mosquito larvae and pupae swim in de-ionized water (cheap, plentiful and easy to make)
size of the stage 4 larvae (largest stage) and pupae is about 3 to 5 mm
about 300 mosquito pupae/larvae in each container (shoe box sized), about 15 containers to be processed each week
sponsor and her team will be in charge of:
checking the correct wavelength of light to make the GFP and CFP fluoresce
testing the ability to infect the genetically modified mosquitoes to further their research
sponsor suggestions:
we can use regular mosquito pupae (alive or dead) to test our risk reduction
better to sort larvae because the pupae can turn into flying adult mosquitoes in 4 hours
need to kill any unused pupae and larvae (usually the males) before discarding for bio-safety
1/15/2020 by email
Genetically modified mosquito eggs have arrived. If they begin the hatching process today (1/15), the pupae will be ready for testing on 1/28 or 1/29.
Instructor Comments from Last Meeting and Actions Taken to Address these Comments (indicate date of comments and if via email or in person)
1/9/2020 in person
need a weekly meeting with sponsor or member of sponsor's team
put names next to tasks
risk reduction (ASAP): test water flow method with floating beads of similar size to pupae
talk to Chris about how to manufacture the channel
if need to use special water for mosquitoes or need to use a lot of water, we must have re-circulation
use a 90 degree gate to control flow of pupae into camera staging area
update instructor about the first meeting with sponsor in winter quarter
Comments from Other Students in the Class (indicate date of comments and if via email or in person)
1/9/2020 in person
need a way to control volume of flow within channel (so that if the gate before the camera is closed, water will not back up
1/10/2020
need a way to ensure that the camera can see the florescent coloration on the eyes of the pupae/larvae
may need to take multiple picture as the mosquito is tumbling down the channel
1/11/2020
CV is out, using light frequency converters now with solenoid valves
Risks and Areas of Concern
Initial holding tank and funnel/hopper: does it work as expected?
Revolving door: does it work as expected?
Resources or Information Required but not Available
Schedule
Describe upcoming milestone
Update Gantt chart.
Budget (list amount spent and amount remaining)
Spent: $0
Remaining: $1800
Progress on Report and Webpage
Completed Week 2 required deliverables on Report
updated pages that were previously missing information
uploaded files that were previously missing from the webpage