Week 2

Overview of Status of Analysis, Design, Fabrication, Tests, etc.

• • Starting to put together ideas for a testing station (to test pipette designs)

    • Need a power supply for the SIXPACK controller (with voltage requirement)

  • Designing mount for manual stage.

  • Purchasing a camera body with C-mount lens attachment (to attach to existing high-power lens)

Accomplishments from Previous Week

• • Met with sponsor, Dr. Matt Strain

  • Identified biggest risks

    • • Pipette provider, sterilization, camera/lens

• • • Started gathering testing equipment

      • XY stage, pipettes (piezo), manual linear stage, camera (BNC), dummy cells, 6 axis controller, magnetic beads.

    • Discussed purchasing an old pipette machine MONA pipetter

• • Met with Dr. Deslon

• • • Discussed more detailed goals

Goals for Next Week (list names after each item). Use specific and measurable objectives.

• • Purchase camera (Laith, Greg, Jonathan)

  • Set up CAD (begin fab?) of testing station (Clark, Fernando)

  • Create prototypes for three pick methods: pipette vacuum, piezo, and paramagnetic beads (Jonathan, Greg, Fernando)

  • Propose a motorized design that includes all three axes (Clark, Laith, Fernando)

Sponsor Comments from Last Meeting and Actions Taken to Address these Comments

• • Sponsor's preferred vendors for different pipettes and capillary tubes.

    • Capillary option will not be possible without tubes smaller than 30 microns in diameter.

  • Sterilization (options to research as a secondary goal)

    • Heating the tip is a good a option

    • Ethanol

    • Plasma 

  • Sent us a a follow up email with linear stages that are available to use in his lab

    • Also sent us an eBay item (~$800+700 shipping) the MONA Micro Pippetter (full 3D pipetter)

Instructor Comments from Last Meeting and Actions Taken to Address these Comments

• More detailed goals and risk factors

  • We detailed out our goals and emailed them to Dr. Delson

• Look at existing linear guides

  • Looked at existing guides; Matt has a motorized stage that we can use (THK w/ Vexta stepper motor)

  • will attempt to set up and study

• High risk area is the pick and place mechanism; how will we test? 

  • Setting up a testing station.

  • Began to get all of the tools we need to test various methods.

    • Still need: Power supply

Risks and Areas of Concern

• • Will the manual stage be testable.

    • Vibrations etc.

  • Testing different methods, will they work? Will they pick up the cell?

  • We need a camera that has USB connection.

Schedule

• • Team Meeting Tues 3-4

  • Dr. Delson meeting Wed. 1-1:30

  • Team meeting Wed. 1:30 - 2ish

  • Sponsor Meeting Thurs 3-4.

  • Team meeting Thrus 4-5ish

Budget (list amount spent and amount remaining)

• • $5000 remaining

  • None spent

Progress on Report and Webpage

• • Week 1 up to date

  • Everyone has posted Individual Component Analyses

  • Gantt chart needs to be uploaded