WEEK 3 PRESENTATION

Post date: Jan 20, 2012 12:44:5 AM

The Design Proposal Presentation lays out where the team is and what is their plan for achieving their design objective during the quarter. It is important to justify design decisions that have already been made, and to identify key design decisions and other challenges that remain.

 Prepare a 10 minute (we will adhere to time limits) presentation that includes:

• 1. A brief overview of the problem definition (1-2 slides).

     1. clearly explain z axis/ methods. clearly explain array wells dimensions and titer - CLARK

  2. List of key design decisions that have already been made, and explain the justification for these decisions. 

  3. "Will attempt to raise and lower the mechanism through a stepper motor/electronic controller assembly."

• • 1. This assembly will be used to test different types of pipettes (pick/place mechanisms)

       1. manual testing stage as a quick testing solution; show picture. (LAITH)

    2. I feel like this should be at the top because it's the only real decision made where everything else are decisions we have not made yet.

    3. purchase camera

• • • 1. show picture

• 4. List key design decisions that still need to be made. Present a plan and timeline for making these decisions before the Proof-of-Concept Presentations in Week 4.

  5. The type of tip that will be used to pick up and dispense the fluid/cells based on testing criteria

• • 1. testing of methods (timeline) (GREG)

       1. magnetic material

       2. pulled glass tips

    2. How will we control the Z stage stepper. (GREG)

    3. Secondary Goals: (FERNANDO) (1 slide)

• • • 1. Sterilization material 

      2. Modifying XY stage.

• 6. pros and cons for every method. (JONATHAN- micro injector with tips,capillary ; paragmagnetic- Fernando; piezo pipette - Greg; hydrophilic tip - Clark; Laith - UV;

• • 1. weeding out methods

    2. eliminated UV method

    3. Tell the anxious crowd which methods we eliminated and which methods we will test! (Laith)

• 7. List major components that need to be purchased, and a plan for placing the purchase orders before, the Proof-of-Concept Presentations in Week 4. Note, some items may have to be purchased earlier due to lead time and project needs. (1 slide)

  8. I think camera should go here; and state we've chosen a preliminary model to test.

• • 1. TIPS (JONATHAN)

       1. pipettes tips

       2. capillary tubes

       3. magnet powder (to be used with PDMS) 

    2. raw materials (JONATHAN)

• • • 1. Material for mounting Z stage.

• • 3. possible buys: stepper motor, coupling (for automating the manual stage) , six pack power supply (JONATHAN)

• 9. List key items that need to be machined, and feedback from shop engineers (Dave Lischer and Tom Chalfant) regarding timeline and feasibility.

• • 1. CAD (CLARK)

       1. Z axis mount

       2. Mount mechanism to microscope stage

• 10. List remaining questions and concerns that need to resolved. I feel like this means more like "questions we don't know how to answer" and not "questions we have"

• • 1. unknown : (LAITH) (1 slide)

       1. unwanted vibrations at the tip of the pick and place mechanism. More generally, how can we be sure we're building something capable of micrometer-level precision?

       2. Which method works best? This just comes down to the testing setup we are already planning.

       3. Will the glass tips be too fragile? Again, we should see this answer fairly quickly with a test once we have the part.

• 11. in light of new information, we may be able to take possesion of the microscope

  12. Show a Gantt chart with task assignment for the rest of the quarter (FERNANDO)