Post date: Jan 11, 2012 11:23:16 PM
- XY Device
. Will we be able to use this on our own? (how it's connected, software involved, etc.)
. Can we see this demonstrated?
- What are ways we can test the experiment without using real HIV-infected cells?
. Is it good enough that we can move regular cells?
(if we can only test iterations of our designs in Matt's lab with his supervision, that's not a lot of time)
. Is it good enough that we move just water?
- How many cells are in each array spot?
. And if yes, in each spot, are ALL cells either HIV-infected or a combination?
. Is the goal to get every cell from one reservoir to the other?
- What devices/items can we use outside of the lab?
- Borrow: pIpet, capulary, manual linear stage, dummy cell, micro array, clamp to hold tool
.
- For these modes from risk reduction: Capillary, PDMS/PEG, and Magnetic
. Do any of these in Matt's opinion have validity?
- Know any way to machine tips for hydrophilic material, PDMS + PEG
- Share combination method: hydrophilic + paramagnetic (Clark)
. We haven't talked much about this past the risk reduction presentation
Is it not a serious option?
- What type of camera does he use if any (Laith)
. Is the camera observing the image under the microscope or is the camera fixed on the array itself directly?
- Prefer sterilization or swap out tips. Sterilization dipping in liquid? drying? cleans the arrays/ titer?
. What does Matt do now for sterilization?
. Does the tip have to be sterilized after every transfer?
. Is a new tip after every transfer realistic or cost-effective?
- Should we also ask Matt's opinion on "what the highest risk" is?
(Our thought was Z-axis; Delsons's was pick-method)
I know you guys can answer some of these, I just wanted to get all my thoughts down on paper. -Greg