発表論文

Kazuyuki Tao, Shin-ichiro Narita, Ui Okada, Satoshi Murakami, Hajime Tokuda. Dissection of an ABC transporter LolCDE function analyzed by photo-crosslinking. J. Biochem. 175: 427-437. 2024 [article]

Yasushi Daimon, Shin-ichiro Narita, Ryoji Miyazaki, Yohei Hizukuri, Hiroyuki Mori, Yoshiki Tanaka, Tomoya Tsukazaki, Yoshinori Akiyama. Reversible auto-inhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation. Proc. Natl. Acad. Sci. USA. 117: 27989-27996. 2020  [article]  [抄訳]

Mohammad Shahrizal, Yasushi Daimon, Yoshiki Tanaka, Yugo Hayashi, Shintaro Nakayama, Shigehiro Iwaki, Shin-ichiro Narita, Hironari Kamikubo, Yoshinori Akiyama, Tomoya Tsukazaki. Structural basis of the function of the β-barrel assembly-enhancing protease BepA.  J. Mol. Biol. 431: 625-635. 2019 [abstract]

Yasushi Daimon, Chigusa Iwama-Masui, Yoshiki Tanaka, Takuya Shiota, Takehiro Suzuki, Ryoji Miyazaki, Hiroto Sakurada, Trevor Lithgow, Naoshi Dohmae, Hiroyuki Mori, Tomoya Tsukazaki, Shin-ichiro Narita, Yoshinori Akiyama. The TPR domain of BepA is required for productive interaction with substrate proteins and the β-barrel assembly machinery (BAM) complex. Mol. Microbiol. 106: 760-776. 2017 [article]

Yasushi Daimon, Shin-ichiro Narita and Yoshinori Akiyama. Activation of toxin-antitoxin system toxins suppresses lethality caused by the loss of σE in Escherichia coli. J. Bacteriol. 197: 2316-2324. 2015 [article]

Asha S. Nayar, Thomas J. Dougherty, Keith E. Ferguson, Brett A. Granger, Lisa McWilliams, Clare Stacey, Lindsey J. Leach, Shin-ichiro Narita, Hajime Tokuda, Alita A. Miller, Dean G. Brown, and Sarah M. McLeod. Novel antibacterial targets and compounds revealed by a high throughput cell wall reporter assay. J. Bacteriol. 197: 1726-1734. 2015 [article]

Sarah M. McLeod, Paul R. Fleming, Kathleen MacCormack, Robert E. McLaughlin, James D. Whiteaker, Shin-ichiro Narita, Makiko Mori, Hajime Tokuda and Alita A. Miller. Small molecule inhibitors of Gram-negative lipoprotein trafficking discovered by phenotypic screening. J. Bacteriol. 197: 1075-1082. 2015 [article]

Yumi Hayashi, Ryoji Tsurumizu, Jun Tsukahara, Kazuki Takeda, Sin-ichiro Narita, Makiko Mori, Kunio Miki and Hajime Tokuda. Roles of the protruding loop of factor B essential for the localization of lipoproteins (LolB) in the anchoring of bacterial triacylated proteins to the outer membrane. J. Biol. Chem. 289: 10530-10539. 2014 [article]

Shin-ichiro Narita, Chigusa Masui, Takehiro Suzuki, Naoshi Dohmae and Yoshinori Akiyama. Protease homolog BepA (YfgC) promotes assembly and degradation of β-barrel membrane proteins in Escherichia coli. Proc. Natl. Acad. Sci. USA. 110: E3612-E3621. 2013 [article]

Masanori Mizutani, Keita Mukaiyama, Jing Xiao, Makiko Mori, Rika Satou, Shin-ichiro Narita, Suguru Okuda and Hajime Tokuda. Functional differentiation of structurally similar membrane subunits of the ABC transporter LolCDE complex. FEBS Lett. 587: 23-29. 2013 [article]

Kazuyuki Tao, Shin-ichiro Narita and Hajime Tokuda. Defective lipoprotein sorting induces lolA expression through the Rcs stress response phosphorelay system. J. Bacteriol. 194: 3643-3650. 2012 [article]

Shin-ichiro Narita and Hajime Tokuda. Overexpression of LolCDE allows the deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase. J. Bacteriol. 193: 4832-4840. 2011 [article]

Chihiro Sakamoto, Rika Satou, Hajime Tokuda and Shin-ichiro Narita. Novel mutations of the LolCDE complex causing outer membrane localization of lipoproteins despite their inner membrane retention signals. Biochem. Biophys. Res. Commun. 401: 586-591. 2010 [abstract]

Kazuyuki Tao, Shoji Watanabe, Shin-ichiro Narita and Hajime Tokuda. A periplasmic LolA derivative with a lethal disulfide bond activates the Cpx stress response system. J. Bacteriol. 192: 5657–5662. 2010 [article]

Yuji Morita, Shin-ichiro Narita, Junko Tomida, Hajime Tokuda and Yoshiaki Kawamura. Application of an inducible system to engineer unmarked conditional mutants of essential genes of Pseudomonas aeruginosa. J. Microbiol. Methods. 82: 205-213. 2010 [abstract]

Jun Tsukahara, Shin-ichiro Narita and Hajime Tokuda. Real time analysis of lipoprotein transfer from LolA to LolB by means of surface plasmon resonance. FEBS Lett. 583: 2987-2990. 2009 [article]

Masaki Yasuda, Asako Iguchi-Yokoyama, Shin-ichi Matsuyama, Hajime Tokuda and Shin-ichiro Narita. Membrane topology and functional importance of the periplasmic region of ABC transporter LolCDE. Biosci. Biotechnol. Biochem. 73: 2310-2316. 2009 [pdf]

Jun Tsukahara, Keita Mukaiyama, Suguru Okuda, Shin-ichiro Narita and Hajime Tokuda. Dissection of the LolB function; lipoprotein binding, membrane targeting, and incorporation of lipoproteins into lipid bilayers. FEBS J. 276: 4496-4504. 2009 [article]

Shin-ichiro Narita and Hajime Tokuda. Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharide. FEBS Lett. 583: 2160-2164. 2009 [article]

Shin-ichiro Narita. Characterization of the diversity of lipoprotein-targeting mechanisms in proteobacteria. Rep. Noda Inst. Sci. Res. 52: 56-57. 2008 [pdf]

Kyoko Kanamaru, Naohiro Taniguchi, Shigehiko Miyamoto, Shin-ichiro Narita and Hajime Tokuda. Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits. FEBS J. 274: 3034-3043. 2007 [article]

Shin-ichiro Narita and Hajime Tokuda. Amino acids at positions 3 and 4 determine the membrane specificity of Pseudomonas aeruginosa lipoproteins. J. Biol. Chem. 282: 13372-13378. 2007 [article]

Shin-ya Tanaka, Shin-ichiro Narita and Hajime Tokuda. Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism. J. Biol. Chem. 282: 13379-13384. 2007 [article]

Hideaki Ito, Atsushi Ura, Yoshihiro Oyamada, Hiroaki Yoshida, Jun-ichi Yamagishi, Shin-ichiro Narita, Shin-ichi Matsuyama and Hajime Tokuda. A new screening method to identify inhibitors of the Lol (localization of lipoproteins) system, a novel antibacterial target. Microbiol. Immunol. 51: 263-270. 2007 [pdf]

Yasuko Ito, Hitomi Matsuzawa, Shin-ichi Matsuyama, Shin-ichiro Narita and Hajime Tokuda. Genetic analysis of the mode of interplay between an ATPase subunit and membrane subunits of the lipoprotein-releasing ATP-binding cassette transporter LolCDE. J. Bacteriol. 188: 2856-2864. 2006 [article]

Hiroyuki Akama, Misa Kanemaki, Masato Yoshimura, Tomitake Tsukihara, Tomoe Kashiwagi, Hiroshi Yoneyama, Shin-ichiro Narita, Atsushi Nakagawa and Taiji Nakae. Crystal Structure of the Drug Discharge Outer Membrane Protein, OprM, of Pseudomonas aeruginosa. J. Biol. Chem. 279: 52816-52819. 2004 [article]

Hiroyuki Akama, Takanori Matsuura, Sachiko Kashiwagi, Hiroshi Yoneyama, Shin-ichiro Narita, Tomitake Tsukihara, Atsushi Nakagawa and Taiji Nakae. Crystal structure of the membrane fusion protein, MexA, of the multidrug transporter in Pseudomonas aeruginosa. J. Biol. Chem. 279: 25939-25942. 2004 [article]

Shin-ichiro Narita, Shima Eda, Eisaku Yoshihara and Taiji Nakae. Linkage of the efflux-pump expression level with substrate extrusion rate in the MexAB-OprM efflux pump of Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 308: 922-926. 2003 [abstract]

Shin-ichiro Narita, Kyoko Kanamaru, Shin-ichi Matsuyama and Hajime Tokuda. A mutation in the membrane subunit of ABC transporter LoICDE complex causing outer membrane localization of lipoproteins against their inner membrane-specific signals. Mol. Microbiol. 49: 167-177. 2003 [article]

Shin-ichiro Narita, Kimie Tanaka, Shin-ichi Matsuyama and Hajime Tokuda. Disruption of lolCDE, encoding an ATP-binding cassette transporter, is lethal for Escherichia coli and prevents release of lipoproteins from the inner membrane. J. Bacteriol. 184: 1417-1422. 2002 [article]

Kenji Nakahigashi, Naoko Kubo, Shin-ichiro Narita, Takeshi Shimaoka, Shimon Goto, Taku Oshima, Hirotada Mori, Maki Maeda, Chieko Wada and Hachiro Inokuchi. HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination. Proc. Natl. Acad. Sci. USA. 99: 1473-1478. 2002 [article]

Toshiharu Yakushi, Kazuhiro Masuda, Shin-ichiro Narita, Shin-ichi Matsuyama and Hajime Tokuda. A new ABC transporter mediating the detachment of lipid-modified proteins from membranes. Nat. Cell Biol. 2: 212-218. 2000 [abstract]

Naoko Kanjo, Shouichi Nishio, Shin-ichiro Narita, Kenji Oeda and Hachiro Inokuchi. Nucleotide sequence of a cDNA clone encoding protoporphyrinogen IX oxidase (Accession No. AB025102) from Soybean. Plant Physiol. 121: 1384. 1999 [article]

Shin-ichiro Narita, Shigeru Taketani and Hachiro Inokuchi. Oxidation of protoporphyrinogen IX in Escherichia coli is mediated by the aerobic coproporphyrinogen oxidase. Mol. Gen. Genet. 261: 1012-1020. 1999 [abstract]

Shin-ichiro Narita, Ryouichi Tanaka, Toshiro Ito, Kiyotaka Okada, Shigeru Taketani and Hachiro Inokuchi. Molecular cloning and characterization of a cDNA that encodes protoporphyrinogen oxidase of Arabidopsis thaliana. Gene 182: 169-175. 1996 [abstract]

総説

Shin-ichiro Narita and Hajime Tokuda. Bacterial lipoproteins; biogenesis, sorting and quality control. Biochim. Biophys. Acta. 1862: 1414-1423. 2017 [article]

成田新一郎. 細菌外膜の生合成および維持機構. 生化学 87: 450-453. 2015 [pdf]

Shin-ichiro Narita. ABC transporters involved in the biogenesis of the outer membrane in gram-negative bacteria. Biosci. Biotechnol. Biochem. 75: 1044-1054. 2011 [pdf]

Shin-ichiro Narita and Hajime Tokuda. Biogenesis and membrane targeting of lipoproteins. A. Böck, R. Curtiss III, J. B. Kaper, P. D. Karp, F. C. Neidhardt, T. Nyström, J. M. Slauch, C. L. Squires, and D. Ussery (ed.), EcoSal—Escherichia coli and Salmonella: Cellular and Molecular Biology. Chapter 4.3.7. ASM Press (Washington, DC, USA) 2010 [article]

Shin-ichiro Narita and Hajime Tokuda. Sorting of bacterial lipoproteins to the outer membrane by the Lol system. A. Economou (ed.), Protein secretion: Methods and Protocols (Methods in Molecular Biology Vol. 619) Chapter 7. Humana Press (Totowa, USA) 2010 [abstract]

Shin-ichiro Narita and Hajime Tokuda. The function of the ABC transporter LolCDE in protein transport to the outer membrane of E. coli. R. E. Dalbey, C. M. Koehler, and F. Tamanoi (ed.), Molecular Machines involved in Protein Transport across Cellular Membranes (The Enzymes Volume XXV) pp.151-174. Academic Press (London, UK) 2007 [abstract]

成田新一郎. βバレル型蛋白質の外膜への挿入・透過をつかさどる蛋白質. 蛋白質核酸酵素 52: 1892. 2007

成田新一郎、徳田元. 大腸菌リポタンパク質の輸送. 実験医学 23: 2283-2288. 2005

Shin-ichiro Narita and Hajime Tokuda. An ABC transporter mediating the membrane detachment of bacterial lipoproteins depending on their sorting signals. FEBS Lett. 580: 1164-1170. 2005 [article]

Shin-ichiro Narita, Shin-ichi Matsuyama and Hajime Tokuda. Lipoprotein trafficking in Escherichia coli. Arch. Microbiol. 182: 1-6. 2004 [abstract]