This device separates colors by wedge prisms rather than mirrors. Under an NIH Phase I Small Business Innovation Research Grant (R43GM112403) we achieved proof of concept. We also demonstrated that the prototypes can measure in vivo calcium activity in C. elegans and spectrally distinguish neurons in Brainbow samples, which express multiple fluorophores. The add-on is predicted to profitably sell at 1/3rd the cost of current instruments, and unlike current instruments, it fits into a standard filter cube with no moving parts, is user-friendly (no alignment or special expertise), and easily scales to more colors. In collaboration with Chroma Technologies, a leading filter company, we are developing second-generation devices with superior imaging.