Publications
Publications
2024
30. A bitopic agonist bound to the dopamine 3 receptor reveals a selectivity site.Arroyo-Urea S, Nazarova AL, Carrión-Antolí Á, Bonifazi A, Battiti FO, Lam JH, Newman AH, Katritch V, García-Nafría J. Nature Communications. 2024. Sep 5;15(1):7759. doi: 10.1038/s41467-024-51993-4
29. DNA Cloning. Watson J.F., Arroyo-Urea S., García-Nafría J. Handbook of Molecular biotechnology. Taylor & Francis. 2024. DOI: 10.1201/9781003055211-8.
28. Comparison of the function of two novel human dopamine D2 receptor variants identifies a likely mechanism for their pathogenicity. Rodriguez-Contreras D, García-Nafría J, Chan AE, Shinde U, Neve KA. Biochem Pharmacol. 2024 Apr 19:116228. doi: 10.1016/j.bcp.2024.116228. Online ahead of print.PMID: 38643909
2023
27. The neuronal calcium sensor NCS-1 regulates the phosphorylation state and activity of the Ga chaperone and GEF Ric-8A.Muñoz-Reyes D, McClelland LJ, Arroyo-Urea S, Sánchez-Yepes S, Sabín J, Pérez-Suárez S, Menendez M, Mansilla A, García-Nafría J, Sprang S, Sanchez-Barrena MJ.Elife. 2023 Nov 29;12:e86151. doi: 10.7554/eLife.86151. Online ahead of print.PMID: 38018500
26. New insights into GPCR coupling and dimerisation from cryo-EM structures.Gusach A, García-Nafría J, Tate CG.Curr Opin Struct Biol. 2023 Mar 22;80:102574. doi: 10.1016/j.sbi.2023.102574. PMID: 36963163
25. Molecular Cloning Using In Vivo DNA Assembly.Arroyo-Urea S, Watson JF, García-Nafría J.Methods Mol Biol. 2023;2633:33-44. doi: 10.1007/978-1-0716-3004-4_3. PMID: 36853454
24. Structural insights into promiscuous GPCR-G protein coupling.Carrión-Antolí A., Mallor-Franco J., Arroyo-Urea S., García-Nafría J.Progress in Molecular Biology and Translational Science. 2023;195:137-152. doi: 10.1016/bs.pmbts.2022.06.015
2021
23. Structure determination of GPCRs: cryo-EM compared with X-ray crystallography.García-Nafría J, Tate CG.Biochem Soc Trans. 2021 Sep 28:BST20210431. doi: 10.1042/BST20210431.
22. Lipoprotein Metabolism in Familial Hypercholesterolemia.Chemello K, García-Nafría J, Gallo A, Martín C, Lambert G, Blom D.J Lipid Res. 2021 Mar 3:100062. doi: 10.1016/j.jlr.2021.100062.
2020
21. Molecular determinants of β-arrestin coupling to formoterol-bound β1-adrenoceptor.Lee Y., Warne T., Nehme R., Pandey S., Chaturvedi M., Dwivedi-Agnihotri H., Edwards P., García-Nafría J., Leslie A., Shukla AK., Tate CG.Nature. 2020 Jun 17. doi: 10.1038/s41586-020-2419-1.
20. Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly. Baumann KN, Piantanida L, García-Nafría J, Sobota D, Voïtchovsky K, Knowles TPJ, Hernández-Ainsa S .ACS Nano. 2020 Feb 25;14(2):2316-2323
19.Cryo-Electron Microscopy: Moving Beyond X-ray Crystal Structures for Drug receptors and Drug development. García-Nafría J.* and Tate CG.* (corresponding author)Annual Reviews in Pharmacology and Toxicology. 2020 Jan 6;60:51-71.
Featured in the atlas of science: https://atlasofscience.org/cryo-electron-microscopy-in-drug-development/
2019
18. In vivo DNA assembly using common laboratory bacteria: a re-emerging tool to simplify molecular cloning.Jake F. Watson and García-Nafría J.Journal of Biological Chemistry. 2019 Oct 18;294(42):15271-15281.
17. Cryo-EM structures of GPCRs coupled to Gs, Gi and Go. García-Nafría J. and Tate CG.Molecular and Cellular Endocrinology. 2019 May 15;488:1-13.
16. Architecture of the heteromeric GluA1/2 AMPA receptor in complex with the auxiliary subunit TARP γ8.Herguedas B, Watson JF, Ho H, Cais O, García-Nafría J, Greger IH. Science. 2019 Mar 14. pii: eaav9011
Highlight in Biofisica Magazine, May 2019.
15. The Molecular Control of Calcitonin Receptor Signaling. dal Maso EG, A.; Zhu, Y.; Garcia-Nafria, J.; Tate, C.G.; Atanasio, S.; Reynolds, C.A.; Ramírez-Aportela, E.; Carazo, JM.; Hick, C.A.; Furness, S.G.B.; Hay, D.L.; Liang, Y.; Miller, L.J.; Christopoulos, A.; Wang, M; Wootten, D.; Sexton, P.M.ACS Pharmacology and Translational Science. 2019, 2:20
14. Druggability Simulations and X-ray Crystallography Reveal a Ligand-binding Site in the GluA3 AMPA Receptor N-terminal Domain. Lee JY, Krieger J, Herguedas B, García-Nafría J, Dutta A, Shaikh SA, Greger IH, Bahar I. (co-first author)Structure. 2019, Feb 5;27(2):241-252.e3.
2018
13. FCHSD2 Promotes Actin Polymerisation at the Base of Clathrin Coated Pits.Almeida-Souza L., Frank R., García-Nafría J., Gunawardana N., Colussi A., Yu M., Howard G., Andrews B., Vallis Y., McMahon H.T.Cell. 2018, Jul 12;174(2):325-337.e14.
12. Cryo-EM structure of the serotonin 5-HT1B receptor coupled to heterotrimeric Go. García-Nafría J.*, Nehme R.*, Edwards P., Tate CG.Nature. 2018, 558 (7711). 620-62
Highlight in Biofisica Magazine, June 2018
11. Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein. García-Nafría J.*, Lee Y.*, Bai X., Carpenter B. & Tate CG. Elife. 2018, May 4;7. pii: e35946.
Highlight in Biofisica Magazine, May 2018
2016
10. Structural dynamics of synaptic AMPA receptors. García-Nafría J, Herguedas B, Watson JF, Greger IH.Journal of Physiology. 2016, Oct 1;594(19):5449-58.
9. IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly. García-Nafria J*, Watson JF, Greger IH. (*correspoding author)Scientific Reports. 2016, Jun 6;6:27459.
8. Structure and organization of heteromeric AMPA-type glutamate receptors. Herguedas B*, García-Nafria J*, Cais O, Fernandez-Leiro R, Ho H, Krieger J, Greger IH.Science. 2016, Apr 29;352(6285):aad3873.
2015
7. Cooperative Dynamics of Intact AMPA and NMDA Glutamate Receptors: Similarities and Subfamily-Specific Differences. Dutta A, Krieger J, Lee JY, García-Nafría J, Greger IH, Bahar I.Structure. 2015, Sep 1;23(9):1692-1704.
2013
6. Tying down the arm in Bacillus dUTPase: structure and mechanism. García-Nafría J, Timm J, Harrison C, Turkenburg JP, Wilson KS.Acta Crystallogr D Biol Crystallogr. 2013, Aug;69(Pt 8):1367-80.
5. Crystal and solution studies reveal that the transcriptional regulator AcnR of Corynebacterium glutamicum is regulated by citrate-Mg2+ binding to a non-canonical pocket.García-Nafría J, Baumgart M, Turkenburg JP, Wilkinson AJ, Bott M, Wilson KS.Journal of Biological Chemistry. 2013, May 31;288(22):15800-12.
2011
4. The structure of Bacillus subtilis SPb prophage dUTPase and its complexes with two nucleotides. García-Nafría J, Harkiolaki M, Persson R, Fogg MJ and Wilson KS.Acta Crystallogr D Biol Crystallogr. 2011, Mar;67(Pt 3):167-75.
2010
3. The structure of the genomic Bacillus subtilis dUTPase: novel features in the Phe-lid. García-Nafría J, Burchell L, Takezawa M, Rzechorzek NJ, Fogg MJ, Wilson KS.Acta Crystallogr D Biol Crystallogr. 2010, Sep;66(Pt 9):953-61.
2. The Corynebacterium glutamicum aconitase repressor: scratching around for crystals. García-Nafría J, Baumgart M, Bott M, Wilkinson AJ, Wilson KS. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010, Sep 1;66(Pt 9):1074-7.
1. Structure of the catalytic domain of the human mitochondrial Lon protease: proposed relation of oligomer formation and activity. García-Nafría J, Ondrovicová G, Blagova E, Levdikov VM, Bauer JA, Suzuki CK, Kutejová E, Wilkinson AJ, Wilson KS. Protein Science. 2010, May;19(5):987-99.
30. A bitopic agonist bound to the dopamine 3 receptor reveals a selectivity site.Arroyo-Urea S, Nazarova AL, Carrión-Antolí Á, Bonifazi A, Battiti FO, Lam JH, Newman AH, Katritch V, García-Nafría J. Nature Communications. 2024. Sep 5;15(1):7759. doi: 10.1038/s41467-024-51993-4
29. DNA Cloning. Watson J.F., Arroyo-Urea S., García-Nafría J. Handbook of Molecular biotechnology. Taylor & Francis. 2024. DOI: 10.1201/9781003055211-8.
28. Comparison of the function of two novel human dopamine D2 receptor variants identifies a likely mechanism for their pathogenicity. Rodriguez-Contreras D, García-Nafría J, Chan AE, Shinde U, Neve KA. Biochem Pharmacol. 2024 Apr 19:116228. doi: 10.1016/j.bcp.2024.116228. Online ahead of print.PMID: 38643909
2023
27. The neuronal calcium sensor NCS-1 regulates the phosphorylation state and activity of the Ga chaperone and GEF Ric-8A.Muñoz-Reyes D, McClelland LJ, Arroyo-Urea S, Sánchez-Yepes S, Sabín J, Pérez-Suárez S, Menendez M, Mansilla A, García-Nafría J, Sprang S, Sanchez-Barrena MJ.Elife. 2023 Nov 29;12:e86151. doi: 10.7554/eLife.86151. Online ahead of print.PMID: 38018500
26. New insights into GPCR coupling and dimerisation from cryo-EM structures.Gusach A, García-Nafría J, Tate CG.Curr Opin Struct Biol. 2023 Mar 22;80:102574. doi: 10.1016/j.sbi.2023.102574. PMID: 36963163
25. Molecular Cloning Using In Vivo DNA Assembly.Arroyo-Urea S, Watson JF, García-Nafría J.Methods Mol Biol. 2023;2633:33-44. doi: 10.1007/978-1-0716-3004-4_3. PMID: 36853454
24. Structural insights into promiscuous GPCR-G protein coupling.Carrión-Antolí A., Mallor-Franco J., Arroyo-Urea S., García-Nafría J.Progress in Molecular Biology and Translational Science. 2023;195:137-152. doi: 10.1016/bs.pmbts.2022.06.015
2021
23. Structure determination of GPCRs: cryo-EM compared with X-ray crystallography.García-Nafría J, Tate CG.Biochem Soc Trans. 2021 Sep 28:BST20210431. doi: 10.1042/BST20210431.
22. Lipoprotein Metabolism in Familial Hypercholesterolemia.Chemello K, García-Nafría J, Gallo A, Martín C, Lambert G, Blom D.J Lipid Res. 2021 Mar 3:100062. doi: 10.1016/j.jlr.2021.100062.
2020
21. Molecular determinants of β-arrestin coupling to formoterol-bound β1-adrenoceptor.Lee Y., Warne T., Nehme R., Pandey S., Chaturvedi M., Dwivedi-Agnihotri H., Edwards P., García-Nafría J., Leslie A., Shukla AK., Tate CG.Nature. 2020 Jun 17. doi: 10.1038/s41586-020-2419-1.
20. Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly. Baumann KN, Piantanida L, García-Nafría J, Sobota D, Voïtchovsky K, Knowles TPJ, Hernández-Ainsa S .ACS Nano. 2020 Feb 25;14(2):2316-2323
19.Cryo-Electron Microscopy: Moving Beyond X-ray Crystal Structures for Drug receptors and Drug development. García-Nafría J.* and Tate CG.* (corresponding author)Annual Reviews in Pharmacology and Toxicology. 2020 Jan 6;60:51-71.
Featured in the atlas of science: https://atlasofscience.org/cryo-electron-microscopy-in-drug-development/
2019
18. In vivo DNA assembly using common laboratory bacteria: a re-emerging tool to simplify molecular cloning.Jake F. Watson and García-Nafría J.Journal of Biological Chemistry. 2019 Oct 18;294(42):15271-15281.
17. Cryo-EM structures of GPCRs coupled to Gs, Gi and Go. García-Nafría J. and Tate CG.Molecular and Cellular Endocrinology. 2019 May 15;488:1-13.
16. Architecture of the heteromeric GluA1/2 AMPA receptor in complex with the auxiliary subunit TARP γ8.Herguedas B, Watson JF, Ho H, Cais O, García-Nafría J, Greger IH. Science. 2019 Mar 14. pii: eaav9011
Highlight in Biofisica Magazine, May 2019.
15. The Molecular Control of Calcitonin Receptor Signaling. dal Maso EG, A.; Zhu, Y.; Garcia-Nafria, J.; Tate, C.G.; Atanasio, S.; Reynolds, C.A.; Ramírez-Aportela, E.; Carazo, JM.; Hick, C.A.; Furness, S.G.B.; Hay, D.L.; Liang, Y.; Miller, L.J.; Christopoulos, A.; Wang, M; Wootten, D.; Sexton, P.M.ACS Pharmacology and Translational Science. 2019, 2:20
14. Druggability Simulations and X-ray Crystallography Reveal a Ligand-binding Site in the GluA3 AMPA Receptor N-terminal Domain. Lee JY, Krieger J, Herguedas B, García-Nafría J, Dutta A, Shaikh SA, Greger IH, Bahar I. (co-first author)Structure. 2019, Feb 5;27(2):241-252.e3.
2018
13. FCHSD2 Promotes Actin Polymerisation at the Base of Clathrin Coated Pits.Almeida-Souza L., Frank R., García-Nafría J., Gunawardana N., Colussi A., Yu M., Howard G., Andrews B., Vallis Y., McMahon H.T.Cell. 2018, Jul 12;174(2):325-337.e14.
12. Cryo-EM structure of the serotonin 5-HT1B receptor coupled to heterotrimeric Go. García-Nafría J.*, Nehme R.*, Edwards P., Tate CG.Nature. 2018, 558 (7711). 620-62
Highlight in Biofisica Magazine, June 2018
11. Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein. García-Nafría J.*, Lee Y.*, Bai X., Carpenter B. & Tate CG. Elife. 2018, May 4;7. pii: e35946.
Highlight in Biofisica Magazine, May 2018
2016
10. Structural dynamics of synaptic AMPA receptors. García-Nafría J, Herguedas B, Watson JF, Greger IH.Journal of Physiology. 2016, Oct 1;594(19):5449-58.
9. IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly. García-Nafria J*, Watson JF, Greger IH. (*correspoding author)Scientific Reports. 2016, Jun 6;6:27459.
8. Structure and organization of heteromeric AMPA-type glutamate receptors. Herguedas B*, García-Nafria J*, Cais O, Fernandez-Leiro R, Ho H, Krieger J, Greger IH.Science. 2016, Apr 29;352(6285):aad3873.
2015
7. Cooperative Dynamics of Intact AMPA and NMDA Glutamate Receptors: Similarities and Subfamily-Specific Differences. Dutta A, Krieger J, Lee JY, García-Nafría J, Greger IH, Bahar I.Structure. 2015, Sep 1;23(9):1692-1704.
2013
6. Tying down the arm in Bacillus dUTPase: structure and mechanism. García-Nafría J, Timm J, Harrison C, Turkenburg JP, Wilson KS.Acta Crystallogr D Biol Crystallogr. 2013, Aug;69(Pt 8):1367-80.
5. Crystal and solution studies reveal that the transcriptional regulator AcnR of Corynebacterium glutamicum is regulated by citrate-Mg2+ binding to a non-canonical pocket.García-Nafría J, Baumgart M, Turkenburg JP, Wilkinson AJ, Bott M, Wilson KS.Journal of Biological Chemistry. 2013, May 31;288(22):15800-12.
2011
4. The structure of Bacillus subtilis SPb prophage dUTPase and its complexes with two nucleotides. García-Nafría J, Harkiolaki M, Persson R, Fogg MJ and Wilson KS.Acta Crystallogr D Biol Crystallogr. 2011, Mar;67(Pt 3):167-75.
2010
3. The structure of the genomic Bacillus subtilis dUTPase: novel features in the Phe-lid. García-Nafría J, Burchell L, Takezawa M, Rzechorzek NJ, Fogg MJ, Wilson KS.Acta Crystallogr D Biol Crystallogr. 2010, Sep;66(Pt 9):953-61.
2. The Corynebacterium glutamicum aconitase repressor: scratching around for crystals. García-Nafría J, Baumgart M, Bott M, Wilkinson AJ, Wilson KS. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010, Sep 1;66(Pt 9):1074-7.
1. Structure of the catalytic domain of the human mitochondrial Lon protease: proposed relation of oligomer formation and activity. García-Nafría J, Ondrovicová G, Blagova E, Levdikov VM, Bauer JA, Suzuki CK, Kutejová E, Wilkinson AJ, Wilson KS. Protein Science. 2010, May;19(5):987-99.