A. Neurophysiology & Cardiovascular Physiology
(Focus: BP, HR, RVLM studies, and in vivo Techniques)
Tail-cuff Methods for BP & HR Monitoring
Blood Pressure Recordings using BIOPAC Systems (MP100 / MP160) to measure PAP & MAP.
ICV (Intracerebroventricular) Implantation
ICV and RVLM Microinjections
Rat Femoral Artery and Vein Cannulation
Blood CSF Collection from Cisterna Magna.
Intracardially Heart Perfusion Techniques
B. Neuropharmacology & Behavioral Neuroscience
(Focus: rodent behavioral paradigms, drug testing, analgesic and anti-inflammatory models)
Hot plate methods
Hole cross test
Tail immersion methods
Open field methods.
Acetic acid-induced writhing test
Comprehensive behavioral tests in mice and rats such as neuropharmacological, analgesic and anti-inflammatory behavioral assays.
C. Neurosurgical & Histological Techniques
(Focus: Advanced surgical skills in rodents)
Brain Stereotaxic Surgery
Brain Slicing and Cryo-sectioning for tissue IHC
Ovarian surgery
Rats/Mice brain dissection
Drosophila Brain & NMJ Dissection
D. Drosophila Genetics & Neuroscience
(Focus: developmental neuroscience & synaptic studies)
Drosophila (Fly) stock maintenance
Drosophila (Fly) Transfer & Sexing Techniques
Drosophila Larvae NMJ Dissection
Drosophila Brain & Tissue Dissection
Brain Stereotaxic Surgery
Brain slicing: Cryo-sectioning of Rats brain for tissue IHC study.
E. Molecular Biology, Biochemistry, & Genetic Technique
(Focus: DNA, RNA, Protein-level assays, genotyping, & molecular cloning)
Genotyping
Bacterial Culture
ELISA techniques
Gel electrophoresis
Polymer chain reaction (PCR)
Western blotting techniques.
Plasmid DNA Extraction / Isolation / Purification
Drosophila sexing (Pupae & Adults)
Drosophila Identification (Male & Female)
F. Cell Biology & Neurobiology
(Focus: cell culture models, Neuron-glia interactions, and in vitro systems)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Primary Cell Culture (Rats/Mice)
Co-culture and Tri-culture systems (Neuron, Astrocyte, Microglia)
Conventional Cell Culture (Cell Line)
Stem Cells Therapy
Confocal Fluorescence Microscopy System
Nikon Eclipse E800 Microscope
V20 Fluorescence Microscope.
EVOS M3000 Imaging System
G. Data Analysis & Imaging Tools
(Focus: image analysis Data processing, & statistical tools)
ImageJ for fluorescence data analysis
GraphPad Prism for Statistical Analysis
Confocal Imaging
High-resolution Imaging for IF/IHC
Data processing for Behavioral & Physiological studies.
H. Instruments & Platforms
Microscope: Confocal Fluorescence Microscopy System, Nikon Eclipse E800 microscope, V20 Fluorescence Microscope, EVOS M3000 Imaging System.
Data Acquisition Systems: BIOPAC MP100 / MP 160
Cryostat: Cryo-sectioning for Brain Slices
Imaging Systems: Fluorescence Imaging Platforms