B.S., Biology- Cornell University 2002
Ph.D., Microbiology and Immunology (Dr. Michele Swanson) - University of Michigan 2006
ACS Postdoctoral Fellow, Molecular and Cell Biology (Dr. Daniel Portnoy) - University of California, Berkeley 2011
Alyssa Brokaw (email)
B.S. Microbiology - University of Minnesota, 2015
Ph.D. Pathobiology (Dr. Lakshmi Rajagopal) - University of Washington, 2024
Project: Drawing on my experience studying pregnancy-associated infections caused by Group B Streptococcus, I aim to identify novel Listeria monocytogenes genes associated with hypervirulent clinical isolates linked to listeriosis during pregnancy. I also hope to exploit Listeria's genetic tractability to further our understanding of how bacterial infections and host inflammation pathways influence pregnancy outcomes and fetal prognosis. Outside of lab, you can find me walking my sweet dog Juniper, testing new recipes, and expanding my collection of fun earrings.
Kevin Parducho (email)
B.S. Microbiology - California State University at Los Angeles, 2016
Ph.D. Cell Biology - Yale University, 2024
Project: Using my background in eukaryotic trafficking pathways, I am investigating mechanisms within phagocytes that govern bacterially-induced production of prostaglandin E2, a critical signaling molecule for the induction of CD8+ T-cell dependent protective immunity. In addition, I am developing a CRISPRi system in L. monocytogenes while unsuccessfully resisting the urge to mention dancing Argentine tango to everyone that I meet.
Medical Scientist Training Program (MSTP)/Cell and Molecular Biology (CMB)
BS, Biochemistry - University of Minnesota, 2018
Ph.D. Cellular and Molecular Biology - University of Wisconsin - Madison 2025
Continuing as a Post-doctoral Researcher in the Sauer Lab for my final year while I complete my M.D.
Project: Our lab primarily studies host-pathogen interactions using the model organism Listeria monocytogenes. I approached understanding this problem through the lens of shared host-pathogen metabolism. During my work I identified the systems L. monocytogenes is primarily using to acquire carbon from the host and characterized mechanisms of defective virulence for a metabolically perturbed strain. I am experienced in bacterial genetics, genetic screens, molecular biology, metabolomics, tissue culture, animal work, and bioinformatics. In my free time I enjoy camping, backpacking, skiing, and cooking.
Cell and Molecular Pathology (CMP)
B.S. Biology and BA English - University of California Los Angeles, 2018
Project:
Microbiology Doctoral Training Program (MDTP)
B.S. Biochemistry and Molecular Biology - Oregon State University, 2023
Project:
Mentor: Matt Freeman
Project: I conducted research in a microbial pathogenesis lab under the supervision of my MD/PhD student mentor. I took on my own project, which focused on the virulence defects of a metabolic mutant in Listeria monocytogenes. I significantly contributed in establishing that pyruvate dehydrogenase complex mutants show an accumulation of pyruvate in Listeria monocytogenes. Further, we determined that this metabolic imbalance directly inhibits phosphotransferase systems and virulence in the host. I learned many new techniques during this project including: bacterial and eukaryotic cell culture, genetic cloning, PCR, metabolomics, modeling infection in cells and mice, and others.
Project:
Project:
Projects: role of DHNA in L. monocytogenes pathogenesis and function of Stk1 in S. aureus beta-lactam resistance
Currently: Associate Professor at National Yang-Ming University
Projects: Role of Stk1 in cell wall stress, redox stress and nutritional stress in S. aureus
Currently: Assistant Professor at University of Missouri, Columbia MO
Projects: Role of PrkA in cell wall stress responses, cytosolic survival and virulence in L. monocytogenes
Currently: Assistant Professor at Medical College of Wisconsin, Milwaukee IWI
Thesis Title: Listeria monocytogenes Adaptations to Cell Wall Stressors inthe Macrophage Cytosol
Currently: Microbiologist - Wisconsin Department of Agriculture, Trade and Consumer Protection , Madison WI
Thesis Title: Modeling Host-Pathogen Interactions Using Zebrafish and Listeria monocytogenes
Currently: Process Scientist at Theragent, Arcadia, CA
Thesis Title: Understanding innate immune signaling during Listeria monocytogenes vaccination and its implications for cancer immunotherapy
Currently: Scientist at Fuji Cellular Dynamics Inc, Madison WI
Thesis Title: Role of DHNA and NAD+/NADH redox balance in L. monocytogenes pathogenesis
Currently: Scientist I at MilliporeSigma, Rockville MD
Thesis Title: Role of eicosaniods on cell-mediated immunity to Listeria monocytogenes and its implications on cancer therapy
Currently: Postdoctoral fellow in the lab of Dr. Marion Pepper, University of Washington
Thesis Title: Maintenance of cell wall integrity in the cytosol of host cells is essential for virulence of Listeria monocytogenes
Currently: Senior Research Scientist, Promega Corp. Madison, WI
Thesis Title: Characterization of the PASTA kinases as antibiotic targets in S. aureus and L. monocytogenes
Currently: Postdoctoral fellow in the lab of Dr. Cameron Currie at McMaster University, Hamilton, ON, CA
Thesis Title: Uncovering the genetic determinants of Listeria monocytogenes survival in the host cytosol
Currently: Scientific Lab Manager at the Carnegie Institute for Science, Pasadena CA
Thesis Title: Innate immune cell recruitment and host defense in response to intracellular bacterial infection
Currently: Microscopy Sales Representative at Evident Scientific, Tempe AZ
Thesis Title: Inflammasome activation impairs cell-mediated immunity to Listeria monocytogenes
Currently: Instructor in Dermatology Harvard Medical School, Boston MA
BS, Microbiology - University of Wisconsin - Madison 2022
Currently: Rotating PhD student, Harvard University
BS, Biochemistry - University of Wisconsin - Madison 2021
Currently: PhD student in the Horswill Lab, University of Colorado, Anschutz Medical Campus
BS, Biochemistry and Microbiology - University of Wisconsin - Madison 2019
Currently: PhD student in the Tait Wojno Lab, University of Washington
BS, Microbiology - University of Wisconsin - Madison 2019
Currently: PhD student in the O'Riordan Lab, University of Michigan Medical School
BS, Biochemistry- University of Wisconsin-Madison 2019
Currently: Medical student at Western Michigan University School of Medicine
Currently: TS/MS Scientist at Eli Lilly, Indianapolis IN
Currently: PhD student in the Biomedical Sciences Graduate Program at the University of California San Francisco (Spitzer Lab)
Currently: PhD student in the Programs in Biomedical Sciences (PIBS) at the University of Michigan - Ann Arbor (O'Riordan Lab)
Currently: PhD student in the Microbiology Doctoral Training program at University of Wisconsin - Madison (Majumder Lab)
Currently: unknown
Currently: Naturalist WisCorps
Currently: Medical Student at Western Michigan University School of Medicine
Currently: unknown
Previously: PhD student in the Microbiology Doctoral Training program at University of Wisconsin - Madison (Sauer Lab)
Currently: Microbiologist - Wisconsin Department of Agriculture, Trade and Consumer Protection , Madison WI
Previously: Tufts University PhD Molecular Microbiology (2022)
Currently: Technology Specialist, Lando & Anastasi, LLP Intellectual Property Law Firm
Previously: Michigan State University PhD in Microbiology and Molecular Genetics (2022)
Currently: Postdoctoral Fellow at the University of Michigan (O'Riordan Lab)
Currently: unknown
Currently: PhD Student in Immunology CU Denver Anschutz Graduate School (Torres Lab)
Currently: PhD student in Epidemiology at the University of Wisconsin-Milwaukee (MPH in Global Health and Epidemiology 2018, George Washington University)
Previously: PharmD Student, UW Madison
Currently: Clinical Pharmacist | Group Health Cooperative of South Central Wisconsin | Patient-Centered Care & Medication Management Expert
Currently: QC analyst at Stratatech Corporation, a Mallinkrodt company
Currently: unknown
Previously: Medical College of Wisconsin
Currently: Orthopedic Surgeon, Medical College of Wisconsin, Milwaukee WI
Previously: Yale University PhD Biomedical and Biological Sciences
Currently: Senior Scientist - Clinical Pharmacology at Parexel