Technical Skills

Human Clinical Samples and Translational Techniques

• Blood-based assays: Plasma and serum preparation; PBMC isolation; CD14⁺    monocyte   separation; immune    marker profiling via multicolor flow cytometry

• Placental tissue: Biopsy processing; cryopreserved single-cell isolation; analysis of immune and cellular markers

Animal Models and In Vivo Techniques (Mice, Rats, Swine)

• Disease modeling and treatment evaluation in murine models (5XFAD,3XFAD, & PGC1a)

• Routes of administration: Intraperitoneal, intravenous, oral gavage, intra-plantar, intra-articular, subcutaneous

• Biological sampling: Retro-orbital bleeding, tissue dissections

• In vivo imaging for disease monitoring and therapeutic efficacy

• Compliance with IACUC and ethical animal handling practices

Gut Microbiome and Microbial Analysis

• Fecal DNA extraction and 16S rRNA gene sequencing

• Short-chain fatty acid (SCFA) and metabolite profiling from feces, serum, and tissues

• Ex vivo/in vitro anaerobic culture of gut bacteria

• Data analysis tools: gutMGene, QIIME2 (basic familiarity), MetaboAnalyst (metabolomics)

• qPCR and antimicrobial sensitivity assays for microbial function assessment

Behavioral Neuroscience Models (Rodents)

• Cognitive & motor assessments: Morris Water Maze, Y-Maze, Novel Object Recognition, Open Field, Rota Rod,      Treadmill

• Pain & anxiety models: Von Frey, Hot Plate, Light/Dark Box, Forced Swim Test, Plethysmometer

Molecular Biology and Immunological Assays

• Genomic DNA and total RNA extraction, RT-PCR, agarose gel electrophoresis

• Western blotting, multicolor flow cytometry (immune phenotyping)

• Immunohistochemistry and immunofluorescence staining of tissues

Cell and Tissue Culture

• Maintenance of primary and established mammalian cell lines under sterile conditions

• Exposure to co-culture and transwell systems

Analytical and Imaging Instrumentation

• Spectroscopy: UV-Vis, zeta potential

• Chromatography: GC-MS, UPLC-MS, HPLC, HPTLC for phytochemical/metabolite profiling

• Microscopy: SEM, TEM, Confocal, Fluorescence microscopy

Language & Software

• Language: English, Hindi

•  Software: Biorender, Graphpad, Basic R, Microsoft packages, Flow Jo, Image J, Mendeley