Alignment Protocols

We provide practical information to build a digital holographic microscope (DHM) using a LED source (e.g., incoherent source) to provide holograms (e.g., interferential fringes) with reduced speckle noise.

We provide practical information to build a polarization-sensitive bright-field microscope to recover the Mueller matrix from 36 intensity-based images. This alignment protocol was written as a part of the collaborative work within Optical Imaging Research Laboratory (OIRL) and Applied Optics Research Group. 

We provide practical information to implement a phase-shifting digital holographic microscope (PS-DHM) using a liquid lens to provide the phase shifting of the interferential fringes. Here, we include the list of the optical and optomechanical components and the alignment recipe of the PS-DHM system.

We present the supplementary information to “Single-shot 3D topography of transmissive and reflective samples with a dual-mode telecentric-based digital holographic microscope". Here, we include the list of optical and optomechanical components from Thorlabs, one of the major vendors, and the optical schematics for each alignment step.

We present the alignment recipe of an off-axis Digital Holographic Microscope (DHM) operating in telecentric regime and transmission-based based on a Mach-Zehnder interferometer. Here, we also include the list of optical and optomechanical components from one of the major vendors of optical elements and optomechanical components.

This document provides practical information implement a fast-axial scanning bright-field microscope using a liquid lens. Here, we include the list of optical and optomechanical components, a description of the design requirements and the alignment protocol to build the widefield fluorescent microscope using a tunable lens.