Legionella and beyond (2000-)

1. Oide H, Kubori T, Nagai H, Nozawa T, Nakagawa I, Sasai M, Yamamoto M, Arasaki K. Phosphatidic acid production on the vacuole harboring Legionella pneumophila is a signal for recognition of interferon-induced GTPases. Proc Natl Acad Sci U S A. 2025 Aug 12;122(32):e2420096122. doi: 10.1073/pnas.2420096122. Epub 2025 Aug 8. PMID: 40779251

2. Yokoyama T, Miyazaki R, Suzuki T, Dohmae N, Nagai H, Tsukazaki T, Kubori T, Akiyama Y. Cleavage cascade of the sigma regulator FecR orchestrates TonB-dependent signal transduction. Proc Natl Acad Sci U S A. 2025 Apr 22;122(16):e2500366122. doi: 10.1073/pnas.2500366122. Epub 2025 Apr 17. PMID: 40244679.

3. Tanaka S, Oide H, Ikeda S, Tagaya M, Nagai H, Kubori T, Arasaki K. Subversion of the host endocytic pathway by Legionella pneumophila-mediated ubiquitination of Rab5. J Cell Biol. 2025 Apr 7;224(4):e202406159. doi: 10.1083/jcb.202406159. Epub 2025 Mar 4. 

4. Pruneda JN, Nguyen JV, Nagai H, Kubori T. Bacterial usurpation of the OTU deubiquitinase fold. FEBS J. 2024 Aug;291(15):3303-3316. doi: 10.1111/febs.16725. Epub 2023 Jan 24. 

5. Kubori Tomoko, Arasaki Kohei, Kitao Tomoe, Nagai Hiroki. Multi-tiered actions of Legionella effectors to modulate host Rab10 dynamics eLife 2024 12:RP89002 https://doi.org/10.7554/eLife.89002.3 

6. Kubori T. A two-component system serves as a central hub for connecting energy metabolism and plasmid dissemination in bacteria. mBio. 2023 Nov 30;14(6):e0247423. doi: 10.1128/mbio.02474-23. Epub ahead of print. PMID: 38032214; PMCID: PMC10746237. 

7. Warren GD, Kitao T, Franklin TG, Nguyen JV, Geurink PP, Kubori T, Nagai H, Pruneda JN. Mechanism of Lys6 poly-ubiquitin specificity by the L. pneumophila deubiquitinase LotA. Mol Cell. 2023 Jan 5;83(1): P105-120.E5 doi: 10.1016/j.molcel.2022.11.022. Epub 2022 Dec 13. PMID: 36538933.

8. Mitsunaka S, Yamazaki K, Pramono AK, Ikeuchi M, Kitao T, Ohara N, Kubori T, Nagai H, Ando H. Synthetic engineering and biological containment of bacteriophages. Proc Natl Acad Sci U S A. 2022 Nov 29;119(48):e2206739119. doi: 10.1073/pnas.2206739119. Epub 2022 Nov 21. PMID: 36409909.

9. Kubori T, Lee J, Kim H, Yamazaki K, Nishikawa M, Kitao T, Oh BH, Nagai H. Reversible modification of mitochondrial ADP/ATP translocases by paired Legionella effector proteins. Proc Natl Acad Sci U S A. 2022 Jun 7;119(23):e2122872119. doi: 10.1073/pnas.2122872119. Epub 2022 Jun 2. PMID: 35653564.

10. Murata M, Kanamori R, Kitao T, Kubori T, Nagai H, Tagaya M, Arasaki K. Requirement of phosphatidic acid binding for distribution of the bacterial protein Lpg1137 targeting syntaxin 17. J Cell Sci. 2022 Mar 15;135(6):jcs259538. doi: 10.1242/jcs.259538. Epub 2022 Mar 30. PMID: 35224642.

11. Kitao T, Kubori T, Nagai H. Recent advances in structural studies of the Legionella pneumophila Dot/Icm type IV secretion system. Microbiol Immunol. 2022; 66: 67– 74. (Epub ahead of print. 2021 Nov 22.) doi: 10.1111/1348-0421.12951.  PMID: 34807482.

12. Takekawa N, Kubori T, Iwai T, Nagai H, Imada K. Structural basis of ubiquitin recognition by a bacterial OTU deubiquitinase LotA. J Bacteriol. 2021 Oct 11:JB0037621. doi: 10.1128/JB.00376-21. Epub ahead of print. PMID: 34633867.

13. 北尾 公英，久堀 智子，永井 宏樹. 病原細菌レジオネラによるユビキチンを介した宿主小胞輸送システムの操作. Journal of Japanese Biochemical Society (生化学) 93(6): 835-839 (2021). doi:10.14952/SEIKAGAKU.2021.930835.

14. Tomoe Kitao, Kohei Arasaki, Hiroki Nagai, and Tomoko Kubori. Protocol for imaging proteins associated with Legionella-containing vacuoles in host cells. STAR Protocols 2, 100410. June 18, 2021 DOI: https://doi.org/10.1016/j.xpro.2021.100410

15. Kawabata M, Matsuo H, Koito T, Murata M, Kubori T, Nagai H, Tagaya M,Arasaki K. Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-Containing vacuole with the ER. PLOS Pathogens 2021, 17(3): e1009437.  doi: https://doi.org/10.1371/journal.ppat.1009437

16. Shimamori, Y., Pramono, A.K., Kitao, T., Suzuki T., Aizawa S-I, Kubori T., Nagai H., Takeda S., Ando H. Isolation and Characterization of a Novel Phage SaGU1 that Infects Staphylococcus aureus Clinical Isolates from Patients with Atopic Dermatitis. Curr Microbiol (2021). doi: https://doi.org/10.1007/s00284-021-02395-y

17. Shimamori, Y.; Mitsunaka, S.; Yamashita, H.; Suzuki, T.; Kitao, T.; Kubori, T.; Nagai, H.; Takeda, S.; Ando, H. Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants. Viruses 2021, 13, 7. doi: 10.3390/v13010007

18. Tomoe Kitao, Kyoichiro Taguchi, Shintaro Seto, Kohei Arasaki, Hiroki Ando, Hiroki Nagai, Tomoko Kubori, Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase, Cell Reports, 2020 Sep 8;32(10):108107. doi: 10.1016/j.celrep.2020.108107

19. Kitao Tomoe, Nagai Hiroki, Kubori Tomoko, Divergence of Legionella Effectors Reversing Conventional and Unconventional Ubiquitination. Frontiers in Cellular and Infection Microbiology 2020 Aug 21;10:448. doi: 10.3389/fcimb.2020.00448

20. Hyunmin Kim, Tomoko Kubori, Kohei Yamazaki, Mi-Jeong Kwak, Suk-Youl Park, Hiroki Nagai, Joseph P Vogel, Byung-Ha Oh. Structural Basis for Effector Protein Recognition by the Dot/Icm Type IVB Coupling Protein Complex. Nat Commun. 2020 May 26;11(1):2623. doi: 10.1038/s41467-020-16397-0.

21. Junya Yamagishi, Kyoko Hayashida, Junji Matsuo, Torahiko Okubo, Makoto Kuroda, Hiroki Nagai, Tsuyoshi Sekizuka, Hiroyuki Yamaguchi, Chihiro Sugimoto. Complete Genome and Bimodal Genomic Structure of the Amoebal Symbiont Neochlamydia Strain S13 Revealed by Ultra-Long Reads Obtained From MinION. J Hum Genet. 2020 Jan;65(1):41-48. doi: 10.1038/s10038-019-0684-3. Epub 2019 Nov 14.

22. Kubori T, Nagai H. Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila. Methods Mol Biol. 1921:241-247. 2019 doi: 10.1007/978-1-4939-9048-1_15.

23. Tomoko Kubori, Tomoe Kitao and Hiroki Nagai, Emerging insights into bacterial deubiquitinases. Current Opinion in Microbiology, 2019-02 | journal-article DOI: 10.1016/j.mib.2018.10.001

24. Hubert Hilbi, Hiroki Nagai, Tomoko Kubori and Craig R. Roy, Subversion of Host Membrane Dynamics by the Legionella Dot/Icm Type IV Secretion System. Current Topics in Microbiolog yand Immunology 413, 2018 2017;413:221-242. DOI: 10.1007/978-3-319-75241-9_9.

25. Kubori T, Kitao-Ando T, Ando H, Nagai H. LotA, a Legionella deubiquitinase, has dual catalytic activity and contributes to intracellular growth. Cell. Microbiol. 2018 e12840. DOI:10.1111/cmi.12840 Selected as an Editor's Choice

26. Okubo, T., M. Matsushita, S. Nakamura, J. Matsuo, H. Nagai and H. Yamaguchi (2018). "Acanthamoeba S13WT relies on its bacterial endosymbiont to backpack human pathogenic bacteria and resist Legionella infection on solid media." Environ Microbiol Rep 10(3): 344-354, 2018, doi:10.1111/1758-2229.12645.

27. Maita, C., M. Matsushita, M. Miyoshi, T. Okubo, S. Nakamura, J. Matsuo, M. Takemura, M. Miyake, H. Nagai and H. Yamaguchi (2018). "Amoebal endosymbiont Neochlamydia protects host amoebae against Legionella pneumophila infection by preventing Legionella entry." Microbes Infect 20(4): 236-244. 2018, doi: 10.1016/j.micinf.2017.12.012.

28. Kubori T, Nagai H. Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila for Negative Stain Electron Microscopy Studies. Bio-protocol Vol 7, Iss 8, April 20, 2017 DOI: 10.21769/BioProtoc.2229

29. Kubori T, Bui XT, Hubber A and Nagai H (2017) Legionella RavZ Plays a Role in Preventing Ubiquitin Recruitment to Bacteria-Containing Vacuoles. Front. Cell. Infect. Microbiol. 7:384. doi: 10.3389/fcimb.2017.00384

30. Hubber A, Kubori T, Coban C, Matsuzawa T, Ogawa M, Kawabata T, Yoshimori T, Nagai H. Bacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis. Sci Rep.2017 Mar 20;7:44795. doi: 10.1038/srep44795.

31. Maita C, Matushita M, Okubo T, Matsuo J, Miyake M, Nagai H, Yamaguchi H. Draft Genome Sequences of Legionella pneumophila JR32 and Lp01 Laboratory Strains Domesticated in Japan. Genome Announc 4, (2016). doi: 10.1128/genomeA.00791-16.

32. Hirayasu K, Saito F, Suenaga T, Shida K, Arase N, Oikawa K, Yamaoka T, Murota H, Chibana H, Nakagawa I, Kubori T, Nagai H, Nakamaru Y, Katayama I, Colonna M and Arase H. Microbially cleaved immunoglobulins are sensed by the innate immune receptor LILRA2. Nat. Microbiol. 1, 16054 (2016). doi: 10.1038/nmicrobiol.2016.54

33. Kubori T,  *Nagai H. The Type IVB secretion system: an enigmatic chimera. Curr Opin Microbiol 29, 22-29 (2016). doi: 10.1016/j.mib.2015.10.001

34. 永井宏樹、病原菌の侵入・増殖と宿主真核細胞の攻防、実験医学 (羊土社), 33, 2778-2783 (2015).

35. Kuroda T, Kubori T, Thanh Bui X, Hyakutake A, Uchida Y, Imada K, *Nagai H. Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion. Sci Rep 5, 10912 (2015). doi: 10.1038/srep10912

36. Kubori T, Hubber AM, *Nagai H. Hijacking the host proteasome for the temporal degradation of bacterial effectors. Methods Mol Biol 1197, 141-152 (2014). doi: 10.1007/978-1-4939-1261-2_8

37. Nagai H. The host-pathogen interaction of Legionella pneumophila. Nihon Saikingaku Zasshi 69, 503-511 (2014).

38. 永井宏樹、レジオネラ症とその病原メカニズムをめぐる新展開、呼吸器内科 (科学評論社), 26, 9-15 (2014).

39. Kubori T, Koike M, Bui XT, Higaki S, Aizawa SI, *Nagai H. Native structure of a type IV secretion system core complex essential for Legionella pathogenesis. Proc Natl Acad Sci U S A 111, 11804-11809  (2014). doi: 10.1073/pnas.1404506111

40. Hubber A, Arasaki K, Nakatsu F, Hardiman C, Lambright D, De Camilli P, Nagai H, Roy CR. The machinery at endoplasmic reticulum-plasma membrane contact sites contributes to spatial regulation of multiple legionella effector proteins. PLoS Pathog 10, e1004222 (2014). doi: 10.1371/journal.ppat.1004222

41. Ishida K, Sekizuka T, Hayashida K, Matsuo J, Takeuchi F, Kuroda M, Nakamura S, Yamazaki T, Yoshida M, Takahashi K, Nagai H, Sugimoto C, Yamaguchi H. Amoebal Endosymbiont Neochlamydia Genome Sequence Illuminates the Bacterial Role in the Defense of the Host Amoebae against Legionella pneumophila. PLoS One 9, e95166 (2014). doi: 10.1371/journal.pone.0095166

42. Hubber A, Kubori T, *Nagai H. Modulation of the ubiquitination machinery by legionella. Curr Top Microbiol Immunol 376, 227-247 (2013). doi:10.1007/82_2013_343

43. Hori JI, Pereira MS, Roy CR, *Nagai H, Zamboni DS. Identification and functional characterization of K(+) transporters encoded by Legionella pneumophila kup genes. Cell Microbiol 15, 2006-2019 (2013). doi:10.1111/cmi.12168

44. Matsuo J, Nakamura S, Ito A, Yamazaki T, Ishida K, Hayashi Y, Yoshida M, Takahashi K, Sekizuka T, Takeuchi F, Kuroda M, Nagai H, Hayashida K, Sugimoto C, Yamaguchi H., Protochlamydia induces apoptosis of human HEp-2 cells through mitochondrial dysfunction mediated by chlamydial protease-like activity factor. PLoS ONE 8(2): e56005. doi:10.1371/journal.pone.0056005 (2013). doi:10.1371/journal.pone.0056005

45. Nagai H, Kubori T. Purification and characterization of legionella u-box-type e3 ubiquitin ligase. Methods Mol Biol 954, 347-354 (2013). doi:10.1007/978-1-62703-161-5_21

46. Tomoko Kubori and Hiroki Nagai. Bacterial effector-involved temporal and spatial regulation by hijack of the host ubiquitin pathway. Front. Microbiol., 2:145. Epub 2011 Jul 4 (2011). doi: 10.3389/fmicb.2011.00145

47. Hiroki Nagai and Tomoko Kubori. Dot/Icm type IVB secretion systems of Legionella and other gram-negative bacteria. Front. Microbiol., 2:136. Epub 2011 Jun 2 (2011). doi: 10.3389/fmicb.2011.00136

48. Tomoko Kubori, Naoaki Shinzawa, Hirotaka Kanuka and Hiroki Nagai, Legionella metaeffector exploits host proteasome to temporally regulate cognate effector. PLoS Pathogens, 6, 12, e1001216 (2010). doi:  10.1371/journal.ppat.1001216

49. Covered as a research highlight in Nat. Rev. Microbiol. 9, 80-81 (February 2011) | doi:10.1038/nrmicro2509

50. Noboru Nakano, Tomoko Kubori, Miki Kinoshita, Katsumi Imada and Hiroki Nagai, Crystal Structure of Legionella DotD: insights into relationship between type IVB and type II/III secretion systems. PLoS Pathogens 6, 10, e1001129 (2010). doi:10.1371/journal.ppat.1001129

51. Covered as a "100 selected papers" in Annual Report 2010-2011 of Osaka University

52. 永井宏樹、病原細菌のIV 型分泌系、日本細菌学雑誌(日本細菌学会)、65：379–386，(2010).

53. 永井宏樹、レジオネラの宿主細胞内動態とエフェクター、実験医学 (羊土社)、27, 1542-1547 (2009).

54. Tomoko Kubori, Akihiro Hyakutake and Hiroki Nagai.  Legionella translocates an E3 ubiquitin ligase that has multiple U-boxes with distinct functions. Mol. Microbiol. 67:1307–1319 (2008). DOI: 10.1111/j.1365-2958.2008.06124.x

55. Hiroki Nagai, Eric D. Cambronne, Jonathan C. Kagan, Juan C. Amor, Richard A. Kahn and Craig R. Roy.  A C-terminal translocation signal required for Dot/Icm-dependent delivery of the Legionella RalF protein to host cells.  Proc. Natl. Acad. Sci. USA.102: 826-831 (2005). doi:10.1073/pnas.0406239101

56. Juan C. Amor, Jennifer Swails, Craig R. Roy, Hiroki Nagai, Alyssa Ingmundson, Xiaodong Cheng and Richard A. Kahn.  The structure of RalF, an ARF guanine nucleotide exchange factor from Legionella pneumophila, reveals the presence of a cap over the active site.  J. Biol. Chem, 280: 1392-1400 (2005). doi:10.1074/jbc.M410820200

57. 永井宏樹、病原細菌のIVB型分泌系と宿主細胞内増殖、蛋白質核酸酵素 (共立出版)、50:44-49 (2005)

58. 永井宏樹、レジオネラと宿主細胞の相互作用、化学療法の領域 (医薬ジャーナル社)、21:1631-1636 (2005)

59. 永井宏樹、レジオネラの感染・増殖の機序を知る—IV型分泌系により宿主細胞中へ輸送されるエフェクターの探索、分子呼吸器病 (先端医学社)、9:490-491 (2005)

60. Hiroki Nagai and Craig R. Roy. Show me the substrates: modulation of host cell function by type IV secretion systems. Cell. Microbiol. 5: 373-383 (2003). DOI: 10.1046/j.1462-5822.2003.00285.x

61. Hiroki Nagai, Jonathan C. Kagan, Xinjun Zhu, Richard A. Kahn, and Craig R. Roy. A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes. Science 295: 697-682 (2002). DOI: 10.1126/science.1067025

62. Covered as a research highlight in Nat. Rev. Mol. Cell Biol. 3, 148 (March 2002) | doi:10.1038/nrm771

63. 永井宏樹、レジオネラ・ニューモフィラ、細菌毒素ハンドブック(櫻井純、本田武司、小熊恵二編 サイエンスフォーラム)、pp 547-549. (2002).

64. Hiroki Nagai and Craig R. Roy. The DotA protein from Legionella pneumophila is secreted by a novel process that requires the Dot/Icm transporter. EMBO J. 20: 5962-5970 (2001). doi: 10.1093/emboj/20.21.5962

65. Jorn Coers, Jonathan C. Kagan, Miguelina Matthews, Hiroki Nagai, Deborah M. Zuckman and Craig R. Roy. Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophilaintracellular growth. Mol. Microbiol. 38: 719-736 (2000). DOI: 10.1046/j.1365-2958.2000.02176.x

E. coli 

1. Ranjan Sen, Hiroki Nagai and Nobuo Shimamoto. Conformational switching of Escherichia coli RNA polymerase-promoter binary complex is facilitated by elongation factor GreA and GreB. Genes to Cells 6: 389-401 (2001).

2. Ranjan Sen, Hiroki Nagai, and Nobuo Shimamoto. Polymerase arrest at the lPR promoter during transcription initiation. J. Biol. Chem. 275: 10899-10904 (2000).

3. Kakoli Mukherjee, Hiroki Nagai, Nobuo Shimamoto, and Dipankar Chatterji. GroEL is involved in activation of Escherichia coli RNA polymerase devoid of the w subunit in vivo. Eur. J. Biochem. 266: 228-235 (1999).

4. Ranjan Sen, Hiroki Nagai and Nobuo Shimamoto. Reduction in abortive transcription from the lPR promoter by mutations in region 3 of the s70 subunit of Escherichia coli RNA polymerase. J. Biol. Chem. 273: 9872-9877 (1998).

5. 永井宏樹 タンパク質フットプリント法 生物物理 (日本生物物理学会) 38: 116-118 (1998).

6.  Hiroki Nagai and Nobuo Shimamoto. Regions of the E. coli primary sigma factor s70 that are involved in interaction with RNA polymerase core enzyme. Genes to Cells 2: 725-734 (1997).

7. Takashi Kinebuchi, Heisaburo Shindo, Hiroki Nagai, Nobuo Shimamoto and Mitsuhiro Shimizu.  Functional domains of E. coli single-stranded DNA binding protein as assessed by analyses of the deletion mutants. Biochemistry 36: 6732-6738 (1997).

8. 永井宏樹 タンパク質フットプリント法 – 新しいタンパク質構造解析法の原理と応用 化学と生物 (日本農芸化学会) 35: 331-332 (1997).

9. Hiroki Nagai, Harumi Yuzawa, Masaaki Kanemori, and Takashi Yura. A distinct segment of the s32 polypeptide is involved in DnaK-mediated negative control of the heat shock response in Escherichia coli. Proc. Natl. Acad. Sci. USA91:10280-10284 (1994).

10. Harumi Yuzawa, Hiroki Nagai, Hirotada Mori, and Takashi Yura. Heat induction of s32 synthesis mediated by mRNA secondary structure: a primary step of the heat shock response in Escherichia coli. Nucleic Acids Res. 21:5449-5455 (1993).

11. Takashi Yura, Hiroki Nagai, and Hirotada Mori. Regulation of the heat-shock response in bacteria. Annu. Rev. Microbiol. 47:321-350 (1993).

12. Takashi Yura, Hirotada Mori, Hiroki Nagai, Toshio Nagata, Akira Ishihama, Nobuyuki Fujita, Katsumi Isono, Kiyoshi Mizobuchi and Atsuo Nakata. Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region. Nucleic Acid Res. 20:3305-3308 (1992).

13. Hiroki Nagai, Harumi Yuzawa and Takashi Yura. Regulation of the heat shock response in E.coli: interplay of positive and negative cis-acting elements in translational control of s32 synthesis.  Biochimie 73:1473-1479 (1991).

14. Hiroki Nagai, Harumi Yuzawa and Takashi Yura. Interplay of two cis-acting mRNA regions in translational control of s32 synthesis during heat shock response of E. coli. Proc. Natl. Acad. Sci. USA 88:10515-10519 (1991).

15. 森浩禎、永井宏樹 原核生物におけるストレス応答の制御機構 細胞工学 (秀潤社) 10:341-347 (1991).

16. 森浩禎、永井宏樹、由良隆 大腸菌のストレス応答 Medical Immunology (国際医書出版) 21:547-552 (1991).

17. Hiroki Nagai, Ryoji Yano, James W. Erickson and Takashi Yura. Transcriptional regulation of the heat shock regulatory gene rpoH in Escherichia coli: involvement of a novel catabolite-sensitive promoter. J. Bacteriol. 172:2710-2715 (1990).

18. Ryoji Yano, Hiroki Nagai, Kiyotaka Shiba and Takashi Yura. A mutation that enhances synthesis of s32 and suppresses temperature-sensitive growth of the rpoH15 mutant of Escherichia coli. J. Bacteriol. 172:2124-2130 (1990).

19.  Takashi Yura, Yasuo Kawasaki, Noriko Kusukawa, Hiroki Nagai, Chieko Wada and Ryoji Yano, Roles and regulation of the heat-shock sigma factor σ32 in Escherichia coli. Antonie van Leeuwenhoek 58, 187-90 (1990).

Salmonella 

1. Marlovits, T. C., Kubori, T., Lara-Tejero M., Thomas, D., Unger, V. M. and Galán, J. E. Assembly of the inner rod determines needle length in the type III secretion injectisome. Nature, 2006. 441:637-640. 

2. Marlovits, T. C., Kubori, T., Sukhan, A., Thomas, D., Galán, J. E. and Unger, V. M. Structural insights into the assembly of the type III secretion needle complex. Science, 2004. 306:1040-2.

3. McKinney, J. S., Zhang, H., Kubori, T., Galán, J. E. and Altman, S. Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences. Nuc. Acids Res., 2004. 32: 848-854. 

4. Kubori, T. and Galán, J. E. Temporal regulation of Salmonella virulence effector function by proteasome-dependent protein degradation. Cell, 2003. 115: 333-342.

5. Sukhan, A., Kubori, T. and Galán, J.E. Synthesis and localization of the Salmonella SPI-1 type III secretion needle complex proteins PrgI and PrgJ J Bacteriol., 2003. 185: 3480-3483. 

6. Russmann, H. Kubori, T., Sauer, J. and Galán, J.E. Molecular and functional analysis of the type III secretion signal of the Salmonella enterica InvJ protein. Mol. Microbiol., 2002. 46: 769-79. 

7. Kubori, T. and Galán, J. E. Salmonella type III secretion-associated protein InvE controls translocation of effector proteins into host cells. J. Bacteriol., 2002. 184: 4699-708. 

8. Sukhan, A., Kubori, T., Wilson, J. and Galán, J.E. Genetic Analysis of Assembly of the Salmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle Complex. J. Bacteriol., 2001. 183: 1159-1167. 

9. Kubori, T., Sukhan, A., Aizawa, S.-I. and Galán, J.E. Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system. Proc. Natl. Acad. Sci. USA, 2000. 97: 10225-10230. 

10. Kubori, T., Matsushima, Y., Nakamura, D., Uralil, J., Lara-Tejero, M.,Sukhan, A., Galán, J.E.* and Aizawa, S.-I.  Supramolecular structure of the Salmonella typhimurium type III proteins secretion system. Science, 1998. 280: 602-605.

11. Aizawa, S.-I. and Kubori, T Bacterial flagellation and cell division. (Review article) Genes to Cells, 1998. 3, 625-634. 

12. Saito, T., Ueno, T., Kubori, T., Yamaguchi, S., Iino, T. and Aizawa, S.-I. Flagellar filament elongation can be impaired by mutations in the hook protein FlgE of Salmonella typhimurium ; A possible role of the hook as a passage for the anti-sigma factor FlgM. Mol. Microbiol., 1998. 27: 1129-1140. 

13. Kubori, T.*, Yamaguchi, S. and Aizawa, S.-I.  Assembly of the switch complex onto the MS ring complex of Salmonella typhimurium does not require any other flagellar proteins. J. Bacteriol., 1997. 179: 813-817.

14. Kubori, T., Shimamoto, N., Yamaguchi, S., Namba, K. and Aizawa, S.-I. Morphological pathway of flagellar assembly in Salmonella typhimurium. J. Mol. Biol., 1992. 226: 433-446.