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BIOTECHNOLOGY 1
BIOTECHNOLOGY 1
Chapter 3
Chapter 3
Objectives:
Objectives:
-Determine the most appropriate tool for measuring specific volumes of masses
-Determine the most appropriate tool for measuring specific volumes of masses
-Describe how to select, set, and use a variety of micropipets within their designated ranges to accurately measure small volumes
-Describe how to select, set, and use a variety of micropipets within their designated ranges to accurately measure small volumes
-Convert between units of measure using the B <- -> S rule and appropriate conversion factors
-Convert between units of measure using the B <- -> S rule and appropriate conversion factors
-Recognize the different expressions for units of concentration measurements and use their corresponding equations to calculate the amount of solute needed to make a specified solution
-Recognize the different expressions for units of concentration measurements and use their corresponding equations to calculate the amount of solute needed to make a specified solution
-Describe what pH is and why it is important in solution preparation
-Describe what pH is and why it is important in solution preparation
Chapter 3 Textbook Material
Chapter 3 Textbook Material
Using SI units and Measuring Volumes
Using SI units and Measuring Volumes
The SI system, also called the metric system, is a series of units that is accepted and used throughout the scientific world.
The SI system, also called the metric system, is a series of units that is accepted and used throughout the scientific world.
There are seven basic units in the SI system: the meter (m), the kilogram (kg), the second (s), the kelvin (K), the ampere (A), the mole (mol), and the candela (cd).
There are seven basic units in the SI system: the meter (m), the kilogram (kg), the second (s), the kelvin (K), the ampere (A), the mole (mol), and the candela (cd).
Calculating Concentrations Video and Practice
Calculating Concentrations Video and Practice
Preparing Solutions - Part 1: Calculating Molar Concentrations
Preparing Solutions - Part 2: Calculating % Concentrations
Preparing Solutions - Part 3: Dilutions from stock solutions
Dilutions
Dilutions
Using Balances
Using Balances
Using Centrifuges (microfuges)
Using Centrifuges (microfuges)
How Centrifugation Works
How Centrifugation Works
Centrifugation is the process where a mixture is separated through spinning. Imagine a mixture where there are two different-sized particles. If you left this mixture out, over time the larger particles would settle to the bottom. Unfortunately, this can take a long time.
Centrifugation uses a centrifuge, or a device that can rapidly spin, to speed up this process. Imagine we put the mixture into test tubes, and those tubes into the centrifuge. The centrifuge holds the top of the tubes, and the bottom is allowed to angle out. As it spins, the larger particles would get flung out farther, and smaller particles would stay close to the center.
IMPROPER USE OF MICROFUGES OR CENTRIFUGES:
Can potentially expose lab workers to hazardous materials as well as severely damage a costly piece of equipment.
PROPER USE OF MICROFUGE/CENTRIFUGE:
Follow procedures as indicated in SOP's.
Wear proper PPE
If you are ever unsure about how to use ANY equipment ALWAYS ask your supervisor/instructor prior to use.
Microtubes come in a variety of sizes. Most common used in labs 0.5mL, 1.5mL and 2.0mL.
Microtubes come in a variety of sizes. Most common used in labs 0.5mL, 1.5mL and 2.0mL.
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