Vaginal Secretions, Lesions & Semen
QUOTE- "The patient is a 57-year-old woman previously diagnosed with Lyme disease who presented with a painful erosive genital lesion. At the time of the outbreak, she was being treated with oral antibiotics, and she tested serologically positive for B burgdorferi and serologically negative for syphilis.
Dieterle-stained biopsy sections revealed visible spirochetes throughout the stratum spinosum and stratum basale, and anti–B burgdorferi immunostaining was positive.
Motile spirochetes were observed by darkfield microscopy and cultured in Barbour-Stoner-Kelly–complete medium inoculated with skin scrapings from the lesion.
Cultured spirochetes were identified genetically as B burgdorferi sensu stricto by polymerase chain reaction, while polymerase chain reaction amplification of treponemal gene targets was negative."
Melissa C. Fesler, FNP-BC*, Marianne J. Middelveen, MDes*, Jennie M. Burke, MSc (Hons) , Raphael B. Stricker, MD
First Published May 1, 2019 Case Report
Link Here
https://doi.org/10.1177/2324709619842901
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“Motile spirochetes were observed in culture fluid inoculated with blood and genital secretions from the three subjects… PCR testing confirmed that the spirochetes isolated from blood and genital secretions were strains of B. burgdorferi.
Journal of Investigative Medicine. 2014; 62:280-281. Isolation and Detection of Borrelia Burdorferi from Human Vaginal and Seminal Secretions, Middelveen MJ, Bandoski C, Burke J, Sapi E, Mayne PJ and Stricker RB.
Stricker RB, Johnson L.
Infect Drug Resist. 2016 Sep 13;9:215-9. doi: 10.2147/IDR.S114770. eCollection 2016.
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Stricker RB, Middelveen MJ.
Expert Rev Anti Infect Ther. 2015;13(11):1303-6. doi: 10.1586/14787210.2015.1081056. Epub 2015 Aug 26.
PMID: 26489537
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Middelveen MJ, Burke J, Sapi E, Bandoski C, Filush KR, Wang Y, Franco A, Timmaraju A, Schlinger HA, Mayne PJ, Stricker RB.
Version 3. F1000Res. 2014 Dec 18 [revised 2015 Jan 1];3:309. doi: 10.12688/f1000research.5778.3. eCollection 2014.