I
Instrumental methods of Bio- Chemical Analysis
Flame photometry: Principle, Theory, Construction of Flame Photometer, General and Clinical applications, study of electrolytes using flame photometer, clinical importance of determination of electrolytes.
New Instrument Automated Electrolyte Analyzer
2.Fluorometry: Definition Principle, Theory and Construction of Fluorometer and its clinical Applications, general and Clinical applications.
II
1.Electrophoresis: Definition and basic principle, Procedure, Different types, and Clinical Applications, Example:- Electrophoretic fractionation of serum protein and Serum lipoproteins.
2.Chromatography: Definition, Basic Principles, Different types, Procedure and Clinical applications.
III.
Immuno Assays – Definition, Basic Principles of Immuno chemical reactions and immune assays.
1.Radio immune assays. Introduction to radioactivity, Radioactive substances and its applications
Ex:- Thyroid Hormone Assays [T3,T4,TSH]
2.Enzyme linked immune sorbent assays (ELISA). Description, Instruments used in these assays, Applications
IV.
Metabolism:
1.Carbohydrate metabolism –Glycolysis, TCA Cycle and Gluconeogenesis.
2.Lipid metabolism- β-Oxidation of Fatty Acids.
3.Protein metabolism – Urea cycle.
V.
Organ Function Tests
VI
Liver Function Tests[ LFT]: Functions of Liver Metabolism of Hb.
Bilirubin Explanation Jaundice and its types Determination of Serum Bilirubin(Vandenberg’s Tests)
4.Determination of total Serum proteins (Albumin and Globulin) & A/G Ratio.
5.Estimation of Enzymes – SGOT, SGPT, Alkaline Phosphatase (ALP) & Acid phosphatase
VII.
Kidney function tests [ KFT/RFT]:
Functions of Kidney Synthesis of Urea Basic Concepts
Estimation of Blood Urea DAM-TSC Method Estimation of Serum Creatinine
[Jaffe’s Method / Alkaline Picrate]
Clearance Test - Definition Creatinine Clearance, Urea Clearance
Urine Examination in accessing KFT
Abnormal Constituents of Urine (Sugar Ketone bodies, Protein Blood bile salts and bile pigments)
VIII.
1.Introduction & Basic concepts.
2.Estimation of Free HCL combined Acids – Clinical significance.
IX.
1.Introduction - Thyroid Gland and its Functions
2.Estimation of Thyroid Hormones [ T3,T4,TSH] -
Methods – RIA & Chemiluminiscence and its
Clinical significance.
X.
Pancreatic Function Test [ PFT]
1.Introduction of Basic concepts.
2.Determination of Serum amylase.
XI.
1.Introduction & Basic concepts of Enzymes, Co-Enzymes, Iso
Enzymes.
2.Importance of Enzymes.
3.Liver Enzymes - SGPT, ALP
and GGT
4.Cardiac Enzymes – CPK, LDH,SGOT.
5.Acid Phosphatase.
XII.
1.Outlines of formations of different body fluids
2.Composition & Analysis of CSF including.
a) CSF Sugar estimation
b) CSF Proteins estimation
c) CSF Chlorides estimation.
Including interpretation of results.
XIII.
Automation of Biochemistry Labs and usage of Computers.
XIV
a) Introduction and importance of quality assurance, General principle.
b) Internal and external quality control.
XV
Diagnostic Tests :
· Blood Glucose
· Blood Urea
· Serum Creatinine
· Plasma Proteins
· Lipid Profile - Serum Total cholesterol, Tri Glycerides HDL Cholesterol, LDL cholesterol and VLDL Cholestrol.
· Glycoselated Haemoglobin
· Serum Calcium and Inorgonic Phosphate
· Arterial Blood Gas Analysis [PH, PO2 AND PCO2 ]