I
Introduction and Scope of Biochemistry Ethics and Disciplines Lab Ethics, Lab Discipline Importance of Patient and Patient Management For Medical Lab Technicians and Medical Lab Organizations. (Notes E/M & T/M) (Q& A)
2.
Reception, Identification, Registration of Bio-Chemical parameters for investigation. (Notes E/M & T/M) Quiz
3.
Glassware and plastic ware used in bio-chemical laboratory. (Notes E/M & T/M)
I.
Glass ware:
a)
Types of glassware, their identification, applications and uses.
b)
Cleaning, drying, maintenance and storage of glassware.
II.
Plastic ware: Brief outline.
4.
Instruments used in Bio-Chemistry Lab:
I. Colorimetry: Visual and photoelectric methods, Design (parts), principles and laws involved construction, operation, Notes E/M or T/M
applications, care and maintenance.
II. Spectrophotometry: Principle, theory, types, construction, operation, applications and Maintenance.
5.
Basic lab operations like-
I. Separation of solids from liquids By .
a) Centrifugation: Principle, Different types of centrifuges, construction of electrical centrifuge, Procedure, care,
maintenance and applications.
b)Filtration – using a funnel.
II. Weighing: Different types of balances – simple balance, electronic balance –
operation, uses, care and maintenance. video Video2 Notes
III. Evaporation
IV. Distillation
V. Refluxing
VI. Drying different salts.
6.
Water Different Types, Chemicals and related substances
I. Purity of chemicals
II. Corrosives.
III. hygroscopic substances Quiz
7.
Physical, Chemical and Biological Hazards Awareness responsibility and measures of safety
Prevention, communication and Control of Lab Hazards
8.
Collection of specimens.
I. Blood – Phlebotomy [ How to draw the blood and precautions], types of Specimens, processing and preservation. Notes (Notes)
II. Urine: Types of Specimens, Collection of 24 hours urine – Preservatives.
9.
10.
Units of measurements.
11.
Solutions.
Types based on solute and solvent, Types based on method of expressing concentration, Percentage calculations. (Notes)
12.
Carbohydrates .
Carbohydrates-Definition, Biological importance, classification, qualitative tests. Notes
13.
Definition, Biological importance, Classification and clinical importance. Notes
14.
Definition, Biological importance of Proteins and Amino Acids Classification,
Qualitative tests. NOTES
15.
Diagnostic Tests:
Blood Sugar- [GOD – POD Method ] Blood urea [ DAM-TSC Method, Enzymatic Method]
Glucose tolerance Test [ GTT] Serum Uric Acid
Serum Creatinine [ Jaffe’s method
/Alkaline picrate method]
16.
Vitamins :
Vitamins classification notes
Fat soluble vitamins, Water Soluble Vitamins, Sources, Daily requirements, Deficiency diseases.
17
Sources, Daily requirements, Deficiency diseases.