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Irene Margiolaki's lab, Department of Biology, University of Patras (UPAT), Greece
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Irene Margiolaki's lab, Department of Biology, University of Patras (UPAT), Greece
  • Development of X-ray Powder Diffraction (XRPD) Methods for Structural Biology (ITCr_H chapter).

  • Protein Structure Analysis via XRPD.

  • Protein expression, purification, crystallization.

  • Electron Diffraction & Crystallography.

  • Crystalline Drug Screening.

  • Structural response of proteins upon variation of their physicochemical environment (relative humidity 1, relative humidity 2, rH and temperature, a related presentation, crystallization conditions, review article etc.).

Cubic Insulin:  I213, a= 78.8598(3) Å, 

ID22, ESRF, λ= 1.29974(2) Å.

Mayaro virus nsP3 macro domain:  

P31, a = 61.603(3) Å, c = 94.619(5) Å, 

2 molecules/ asu, 58% solvent content

ID14- 2, PILATUS area detector, λ=0.9934 Å.

Humidity induced phase transitions of HEW lysozyme 

investigated by microcrystalline powder diffraction.

Mayaro virus nsP3 macro domain:  

P31, a = 61.603(3) Å, c = 94.619(5) Å, 

Expression, Purification and Crystallization

The pharmaceutical protein:

 Urate Oxidase.

The High Resolution Powder Diffraction Beamline ID31- ESRF (Grenoble)

(Moved to ID22).

            In Quest for Improved Microcrystalline Drugs against Diabetes: Insulin complexed with selected phenol-based ligands

In situ detection of a novel lysozyme monoclinic

crystal form upon controlled relative humidity

variation

Novel characterization techniques 

for cultural heritage using a TEM orientation 

imaging in combination with 3D precession 

diffraction tomography: a case study of yellow ancient Roman glass tesserae

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