Ryo Matsuda, Masayoshi Sano, Chiho Tsuchiya, Keigo D. Takahashi, Shintaro Takao, Kohei Mizobata, Masato Moteki, Ryosuke Makabe, Norio Kurosawa. Spatial and temporal occurrence of two Gyrodinium species in the seasonal ice zone off Wilkes Land, Southern Ocean. Polar Science. 2025 Jan :101169. doi: 10.1016/j.polar.2025.101169.

Hiroyuki D. Sakai, Naswandi Nur, Antonius Suwanto, Norio Kurosawa. Complete genome sequence of strain KD-1T, the type strain of Sulfurisphaera javensis, isolated from an Indonesian hot spring. Microbiol Resour Announc. 2024 Oct 29;13(12):e0066424. doi: 10.1128/mra.00664-24.

Ayirkm Adugna Woldie, Anupreet Kaur Chowdhary, Mutsumi Sekine, Masatoshi Kishi, Mankul Beshi Zegeye, Norio Kurosawa, Tatsuki Toda. Growth characteristics and molecular identification of indigenous Limnospira strains from Ethiopian soda lakes as a protein source. Biocatalysis and Agricultural Biotechnology. 2024 Sep 60:103336.

Satoshi Nakagawa, Hiroyuki D Sakai, Shigeru Shimamura, Yoshiki Takamatsu, Shingo Kato, Hirokazu Yagi, Saeko Yanaka, Maho Yagi-Utsumi, Norio Kurosawa, Moriya Ohkuma, Koichi Kato, Ken Takai. N-linked protein glycosylation in Nanobdellati (formerly DPANN) archaea and their hosts. J Bacteriol. 2024 Sep 19;206(9):e0020524.  doi: 10.1128/jb.00205-24.  Epub 2024 Aug 28.

Matthew D Johnson, Doulin C Shepherd, Hiroyuki D Sakai, Manasi Mudaliyar, Arun Prasad Pandurangan, Francesca L Short, Paul D Veith, Nichollas E Scott, Norio Kurosawa, Debnath Ghosal. Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system. Nat Commun. 2024 Aug 16;15(1):7066.  doi: 10.1038/s41467-024-51159-2.

Matthew D Johnson, Hiroyuki D Sakai, Bindusmita Paul, Takuro Nunoura, Somavally Dalvi, Manasi Mudaliyar, Doulin C Shepherd, Michiru Shimizu, Shubha Udupa, Moriya Ohkuma, Norio Kurosawa, Debnath Ghosal. Large attachment organelle mediates interaction between Nanobdellota archaeon YN1 and its host. ISME J. 2024 Jan 8;18(1):wrae154.  doi: 10.1093/ismejo/wrae154.

Syun-ichi Urayama, Akihito Fukudome, Miho Hirai, Tomoyo Okumura, Yosuke Nishimura, Yoshihiro Takaki, Norio Kurosawa, Eugene V Koonin, Mart Krupovic, Takuro Nunoura. Double-stranded RNA sequencing reveals distinct riboviruses associated with thermoacidophilic bacteria from hot springs in Japan. Nat Microbiol. 2024 Feb;9(2):514-523.  doi: 10.1038/s41564-023-01579-5.  Epub 2024 Jan 17.

Ryo Matsuda, Ryosuke Makabe, Masayoshi Sano, Shintaro Takao, Masato Moteki, Norio Kurosawa. Fecal Pellet‐Like Gyrodinium Species in Sinking Particles: Newly Found Potential Contributors for Carbon Export in the Antarctic Seasonal Ice Zone. Journal of Geophysical Research: Biogeosciences. 2023;128 (10), e2023JG007705.

Daiki Nomura, Reishi Sahashi, Keigo D Takahashi, Ryosuke Makabe, Masato Ito, Manami Tozawa, Pat Wongpan, Ryo Matsuda, Masayoshi Sano, Michiyo Yamamoto-Kawai, Natsumi Nojiro, Aiko Tachibana, Norio Kurosawa, Masato Moteki, Takeshi Tamura, Shigeru Aoki, Hiroto Murase. Biogeochemical characteristics of brash sea ice and icebergs during summer and autumn in the Indian sector of the Southern Ocean. Progress in Oceanography. 2023;214, 103023.

Syun-ichi Urayama, Akihito Fukudome, Miho Hirai, Tomoyo Okumura, Yosuke Nishimura, Yoshihiro Takaki, Norio Kurosawa, Eugene V Koonin, Mart Krupovic, Takuro Nunoura. Distinct groups of RNA viruses associated with thermoacidophilic bacteria. bioRxiv, 2023.07. 02.547447.

Shoji Suzuki and Norio Kurosawa. The impact of single-stranded DNA-binding protein SSB and putative SSB-interacting proteins on genome integrity in the thermophilic crenarchaeon Sulfolobus acidocaldarius. Int J Mol Sci. 2023 Feb 25;24(5):4558. doi: 10.3390/ijms24054558.

Masayoshi Sano, Ryosuke Makabe, Ryo Matsuda, Norio Kurosawa, Masato Moteki. Effectiveness of Lugol’s iodine solution for long-term preservation of zooplankton samples for molecular analysis. Plankton and Benthos Research. 2022;17(4) 349-357.

Hiroyuki D. Sakai, Koichi Nakamura, Norio Kurosawa. Stygiolobus caldivivus sp. nov., a facultatively anaerobic hyperthermophilic archaeon isolated from the Unzen hot spring in Japan. Int J Syst Evol Microbiol. 2022 Aug;72(8).  doi: 10.1099/ijsem.0.005486.

小林真輝, 髙山佳樹, 下出信次, 戸田龍樹, 黒沢則夫. カイアシ類1 個体からのDNA 抽出方法の改良とホルマリン固定期間がミトコンドリア遺伝子のPCR増幅に与える影響. Bull. Plankton Eco-Eng. Res. 2022; 2: 56-65.

Maria Cecilia D. Salangsang, Mutsumi Sekine, Shinichi Akizuki, Hiroyuki D. Sakai, Norio Kurosawa, Tatsuki Toda. Effect of carbon to nitrogen ratio of food waste and short resting period on microbial accumulation during anaerobic digestion. Biomass and Bioenergy, 2022 Jul;162 106481.

Hiroyuki D. Sakai, Norio Kurosawa.Complete Genome Sequence of the Hyperthermophilic and Acidophilic Archaeon Saccharolobus caldissimus Strain HS-3T. Microbiol Resour Announc. 2022 Apr 21;11(4):e0107821. doi: 10.1128/mra.01078-21. 

Hiromi Omokawa, Norio Kurosawa, Hiroyuki D Sakai. Complete Genome Sequence of Acidianus sp. Strain HS-5, Isolated from the Unzen Hot Spring in Japan.  Microbiol Resour Announc. 2022 Feb 17;11(2):e0115921. doi: 10.1128/mra.01159-21.

Hiroyuki D. Sakai, Naswandi Nur, Shingo Kato, Masahiro Yuki, Michiru Shimizu, Takashi Itoh, Moriya Ohkuma, Antonius Suwanto, Norio Kurosawa. Insight into the symbiotic lifestyle of DPANN archaea revealed by cultivation and genome analyses. Proc Natl Acad Sci USA. 2022 Jan 18;119(3):e2115449119. doi: 10.1073/pnas.2115449119.

Ryo Matsuda, Shoji Suzuki, Norio Kurosawa. Genetic study of four candidate Holliday junction processing proteins in the thermophilic crenarchaeon Sulfolobus acidocaldarius. Int. J. Mol. Sci. 2022 Jan 9;23(2):707. doi: 10.3390/ijms23020707.

Shoji Suzuki, Norio Kurosawa, Takeshi Yamagami, Shunsuke Matsumoto, Tomoyuki Numata, Sonoko Ishino, Yoshizumi Ishino. Genetic and biochemical characterizations of aLhr1 helicase in the thermophilic crenarchaeon Sulfolobus acidocaldarius. Catalysts. Dec 29;12(1):34 (2021)

Takenori Satomura, Kohei Uno, Norio Kurosawa, Haruhiko Sakuraba, Toshihisa Ohshima, Shin-Ichiro Suye. Characterization of a Novel Thermostable Dye-Linked l-Lactate Dehydrogenase Complex and Its Application in Electrochemical Detection. Int. J. Mol. Sci. Dec 17;22(24):13570 (2021)

Hiroyuki D. Sakai, Ryo Matsuda, Satoshi Imura, Norio Kurosawa. Complete genome and plasmid sequences of the psychrotolerant Aureimonas strain SA4125, isolated from Antarctic moss vegetation. Microbiology Resource Announcements. Oct 14;10(41):e0087821 (2021)

Koichi Nakamura, Norio Kurosawa, Hiroyuki D. Sakai. Complete genome sequence of a novel thermoacidophilic and facultatively aerobic archaeon, Stygiolobus sp. strain KN-1. Microbiology Resource Announcements. Sep 30;10(39):e0058121 (2021)

Hiromi Omokawa, Norio Kurosawa, Shingo Kato, Takashi Itoh, Moriya Ohkuma, Hiroyuki D. Sakai. Complete genome sequence of a novel Sulfolobales archaeon strain HS-7 isolated from Unzen Hot Spring in Japan. Microbiology Resource Announcements. Sep 23;10(38):e0058221 (2021)

宮林裕香, 坪井和彦, 酒井博之, 黒沢則夫, NUR Naswandi, SUWANTO Antonius. 集積培養と16S rRNA遺伝子クローン解析法によるインドネシアの温泉からの培養可能な新規好熱菌の探索. 温泉科学. 71(1) 38-49 (2021)

Miyabayashi H, Sakai HD, Kurosawa N.
DNA polymerase B1 binding protein 1 is important for DNA repair by holoenzyme PolB1 in the extremely thermophilic crenarchaeon sulfolobus acidocaldarius.
Microorganisms. 2021 Feb;9(2):1-14 [LINK] 

Rahmadina Radita, Antonius Suwanto, Norio Kurosawa, Aris Tri Wahyudi, Iman Rusmana. Decomposition of Rice Chaff Using a Cocultivation System of Thermobifida fusca and Ureibacillus thermosphaericus , Proceedings, 66( 1), 31- 31, (2021)

Rahmadina Radita, Antonius Suwanto, Norio Kurosawa, Aris Tri Wahyudi, Iman Rusmana. Dinamics of microbialcommunity during tempeh fermentation. Biotropia. Apr. 28(1):11-20 (2021)

Miyabayashi H, Jain R, Suzuki S, Grogan D.W, Kurosawa N.
PolB1 Is Sufficient for DNA Replication and Repair Under Normal Growth Conditions in the Extremely Thermophilic Crenarchaeon Sulfolobus acidocaldarius.
Front. Microbiol. 2020 Dec;11 [LINK] 

Tachihana S, Nagao N, Katayama T, Hirahara M, Yusoff F.M, Banerjee S, Shariff M, Kurosawa N, Toda T, Furuya, K.
High Productivity of Eicosapentaenoic Acid and Fucoxanthin by a Marine Diatom Chaetoceros gracilis in a Semi-Continuous Culture.
Front. Bioeng. Biotechnol. 2020 Dec;8

Sano M, Makabe R, Kurosawa N, Moteki M, Odate T.
Effects of Lugol's iodine on long-term preservation of marine plankton samples for molecular and stable carbon and nitrogen isotope analyses.
LIMNOL OCEANOGR-METH. 2020 Nov;18(11):635-643

Satomura T, Emoto S, Kurosawa N, Ohsima T, Sakuraba H, Suye S.-I.
Characterization of dye-linked D-amino acid dehydrogenase from Sulfurisphaera tokodaii expressed using an archaeal recombinant protein expression system.
J Biosci and Bioeng. 2020 Sep;130(3):247-252

杉森賢司, 黒沢則夫, 長島秀行.
日本の温泉微生物について ～概要～.
温泉科学 70(1) 2 - 11 (2020)

Sekine M, Akizuki S, Kishi M, Kurosawa N, Toda T.
Simultaneous biological nitrification and desulfurization treatment of ammonium and sulfide-rich wastewater: Effectiveness of a sequential batch operation.
Chemosphere. 2020 Apr;244

Suzuki S, Kurosawa N.
Endonucleases responsible for DNA repair of helix-distorting DNA lesions in the thermophilic crenarchaeon Sulfolobus acidocaldarius in vivo.
Extremophiles. 2019 Sep;23(5):613-624 [LINK] 

Sakai HD, Kurosawa N.
Complete genome sequence of the Sulfodiicoccus acidiphilus strain HS-1T, the first crenarchaeon that lacks polB3, isolated from an acidic hot spring in Ohwaku-dani, Hakone, Japan.
BMC Research Notes. 2019 Jul;12(1) [LINK] 

Chaya A, Kurosawa N, Kawamata A, Kosugi M, Imura S.
Community structures of bacteria, archaea, and eukaryotic microbes in the freshwater glacier lake Yukidori-Ike in Langhovde, East Antarctica.
Diversity. 2019 Jul;11(7):15

Suzuki S, Kurosawa N.
Robust growth of archaeal cells lacking a canonical single-stranded DNA-binding protein.
FEMS Microbiol Letters. 2019 Jun;366(10) [LINK] 

Suzuki S, Kurosawa N.
Participation of UV-regulated genes in the response to helix-distorting DNA damage in the thermoacidophilic crenarchaeon sulfolobus acidocaldarius.
Microbes and Evol. 2019;34(4):363-373 [LINK] 

梶原鈴加, 黒沢則夫.
草津温泉湯畑に生息する微生物の集積培養と新規好酸性細菌の分離.
温泉科学. 2019; 68(4):252-260

Kosugi M, Maruo F, Inoue T, Kurosawa N, Kawamata A, Koike H, Kamei Y, Kudoh S, Imura S.
A comparative study of wavelength-dependent photoinactivation in photosystem II of drought-tolerant photosynthetic organisms in Antarctica and the potential risks of photoinhibition in the habitat.
Annals of Botany. 2018 Dec;122(7):1263-1278

Radita R, Suwanto A, Kurosawa N, Wahyudi AT, Rusmana I.
Firmicutes is the predominant bacteria in tempeh. International Food Research Journal.
2018 Dec;25(6):2313-2320.

Goto M, Nagao N, Yusoff F.M, Kamarudin M.S, Katayama T, Kurosawa N, Koyama M, Nakasaki K, Toda T.
High ammonia tolerance on growth rate of marine microalga Chlorella vulgaris.
J Evol Biol. 2018 Sep;39(5):843-848

黒澤佳子，酒井博之，黒沢則夫.
雲仙温泉に生息する好熱菌の非培養法による種組成解析.
温泉科学. 2018 Sep;69(2):81-89.

黒沢則夫. 温泉の生物資源 ～好熱菌～. 温泉科学. 2018 Sep;68(2):65.

Tsuboi K, Sakai HD, Nur N, Stedman KM, Kurosawa N, Suwanto A.
Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.
Int J Syst Evol Microbiol. 2018 Jun;68(6):1907-1913.  [LINK]

Sakai HD, Kurosawa N.
Saccharolobus caldissimus gen. nov., sp. nov., a facultatively anaerobic iron-reducing hyperthermophilic archaeon isolated from an acidic terrestrial hot spring, and reclassification of Sulfolobus solfataricus as Saccharolobus solfataricus comb. nov. and Sulfolobus shibatae as Saccharolobus shibatae comb. nov.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1271-1278. [LINK]

Radita R, Suwanto A, Kurosawa N, Wahyudi AT, Rusmana I.
Metagenome analysis of tempeh production: Where did the bacterial community in tempeh come from? Malaysian Journal of Microbiology. 2017 Dec;13:280-288. [LINK]

Suzuki S, Kurosawa N.
Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius.
Archaea. 2017 Oct 31;2017:7459310. [LINK]

Watanabe K, Koyama M, Ueda J, Ban S, Kurosawa N, Toda T.
Effect of operating temperature on anaerobic digestion of the Brazilian waterweed Egeria densa and its microbial community.
Anaerobe. 2017 Oct;47:8-17. [LINK]

Matsui M, Kawamata A, Kosugi M, Imura S, Kurosawa N.
Diversity of proteolytic microbes isolated from Antarctic freshwater lakes and characteristics of their cold-active proteases.
Polar Science. 2017 Sep;13:82-90. [LINK]

Koyama M, Watanabe K, Kurosawa N, Ishikawa K, Ban S, Toda T.
Effect of alkaline pretreatment on mesophilic and thermophilic anaerobic digestion of a submerged macrophyte: Inhibition and recovery against dissolved lignin during semi-continuous operation.
Bioresource Technology. 2017 Aug;238:666-674. [LINK]

Sakai HD, Kurosawa N.
Sulfodiicoccus acidiphilus gen. nov., sp. nov., a sulfur-inhibited thermoacidophilic archaeon belonging to the order Sulfolobales isolated from a terrestrial acidic hot spring.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1880-1886. [LINK]

Imaizumi Y, Nagao N, Yusoff FM, Kurosawa N, Kawasaki N, Toda T.
Lumostatic operation controlled by the optimum light intensity per dry weight for the effective production of Chlorella zofingiensis in the high cell density continuous culture.
Algal Research. 2016. 20:110-117. [LINK]

Sakai HD, Kurosawa N.
Exploration and isolation of novel thermophiles in frozen enrichment cultures derived from a terrestrial acidic hot spring.
Extremophiles. 2016 Mar;20(2):207-14. [LINK]

Suzuki S, Kurosawa N.
Disruption of the gene encoding restriction endonuclease SuaI and development of a host-vector system for the thermoacidophilic archaeon Sulfolobus acidocaldarius.
Extremophiles. 2016 Mar;20(2):139-48. [LINK]

Satoh T, Yamamoto S, Kurosawa N.
Chemical Characteristics of Solfataric-Acidic Pond Waters in the Kirishima Geothermal Area, Kagoshima Prefecture.
Journal of Hot Spring Sciences. 2015. 63:100-17.

Kosugi M, Kurosawa N, Kawamata A, Kudoh S, Imura S.
Year-round micrometeorological data from the habitats of terrestrial photosynthetic organisms in Langhovde, East Antarctica, during 2013. JARE data reports. 2015.
Terrestrial biology 8:1-6.

Ueda J, Kurosawa N.
Characterization of an extracellular thermophilic chitinase from Paenibacillus thermoaerophilus strain TC22-2b isolated from compost.
World J Microbiol Biotechnol. 2015 Jan;31(1):135-43.

Kok SP, Tsuchiya K, Komatsu K, Toda T, Kurosawa N.
The protistan microplankton community along the Kuroshio Current revealed by 18S rRNA gene clone analysis: a case study of the differences in distribution interplay with ecological variability.
Plankton and Benthos Research. 2014 May;9(2):71-82

Satoh T, Watanabe K, Yamamoto H, Yamamoto S, Kurosawa N.
Bacterial Community Structures in the Solfataric-Acidic Ponds in the Kirishima Geothermal Area, Kagoshima Prefecture.
Journal of Hot Spring Sciences. 2013 Sep;63(2):100-17.

Nishiyama M, Yamamoto S, Kurosawa N.
Microbial community analysis of a coastal hot spring in Kagoshima, Japan, using molecular- and culture-based approaches.
J Microbiol. 2013 Aug;51(4):413-22.

Satoh T, Watanabe K, Yamamoto H, Yamamoto S, Kurosawa N.
Archaeal community structures in the solfataric acidic hot springs with different temperatures and elemental compositions.
Archaea. 2013;2013:723871.

Ueda J, Yamamoto S, Kurosawa N.
Paenibacillus thermoaerophilus sp. nov., a moderately thermophilic bacterium isolated from compost.
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3330-5.

Nagao N, Tajima N, Kawai M, Niwa C, Kurosawa N, Matsuyama T, Yusoff FM, Toda T.
Maximum organic loading rate for the single-stage wet anaerobic digestion of food waste. Bioresour Technol. 2012 Aug;118:210-8.

Kok SP, Kikuchi T, Toda T, Kurosawa N.
Diversity analysis of protistan microplankton in Sagami Bay by 18S rRNA gene clone analysis using newly designed PCR primers.
J Oceanogr. 2012 Oct;(68): 599-613.

Kok SP, Kikuchi T, Toda T, Kurosawa N.
Diversity and community dynamics of protistan microplankton in Sagami Bay revealed by 18S rRNA gene clone analysis.
Plankton Benthos Res. 2012 May;7(2): 75-86.

Kawasaki Y, Aoki M, Makino Y, Sakai H, Tsuboi Y, Ueda J, Sonoda K, Watanabe K, Yamamoto S, Kurosawa N.
Characterization of moderately thermophilic bacteria isolated from saline hot spring. Microbiology Indonesia. 2011 June;5(2):56-60.

Kimata-Kino N, Ikeda S, Kurosawa N, Toda T.
Saline adaptation of granules in mesophilic UASB reactors.
International Biodeterioration and Biodegradation. 2011 Jan;65(1):65-72.

Kurosawa N, Sato S, Kawarabayasi Y, Imura S, Naganuma T.
Archaeal and bacterial community structures in the anoxic sediment of Antarctic meromictic lake Nurume-Ike.
Polar Science. 2010 Aug;4(2):421-429.

Watanabe K, Nagao N, Toda T, Kurosawa N.
Bacterial community in the personal-use composting reactor revealed by isolation and cultivation-independent method.
Journal of Environmental Science and Health B. 2010 Jul;45(5):372-378.

Watanabe K, Nagao N, Toda T, Kurosawa N.
The dominant bacteria shifted from the order Lactobacillales to Bacillales and Actinomycetales during a start-up period of large-scale, completely-mixed composting reactor using plastic bottle flakes as bulking agent.
World Journal of Microbiology and Biotechnology. 2009. 25:803-811.

Watanabe K, Nagao N, Osa S, Matsuyama T, Kurosawa N, Toda T.
Bacterial community and decomposition rate in long term fed-batch composting using woodchip and Polyethylene terephthalate (PET) as bulking agents.
World Journal of Microbiology and Biotechnology. 2008. 24(8):1417-1424.

Watanabe K, Nagao N, Toda T, Kurosawa N.
Changes in bacterial communities accompanied by aggregation in a fed-batch composting reactor.
Current microbiology. 2008. 56(5):458-467.