PhD Graduate Assistant

PhD research assistant (August 2014 – August 2018)

Department of Anatomy and Cell Biology, University of Florida, FL 32603.

Advisor: Daiqing Liao, Ph.D. (https://physiology.med.ufl.edu/profile/liao-daiqing/)

• From 2014 to 2018: My research efforts contributed to 6 peer-reviewed manuscripts where 4 were first authored. 

• Designed, conducted, and significantly contributed to multiomics projects to discover biomarkers, signaling mechanism, and novel therapeutics. Examples include: 

• Skilled with High-Pressure Liquid Chromatography coupled High Resolution Mass Spectrometry (UHPLC-HRMS)- based metabolomics/lipidomics (Mahmud et al., Analytical & Bioanalytical Chemistry, 2017, PMID: 28844081)

• Discovered novel role of DAXX in de novo lipogenesis of triple negative breast and other cancer (Mahmud et al., Nature Communications, 2023)

• Discovered DAXX as an oncogene in 18 human cancers (Mahmud et al., Nucleic acid Res, 2019)

Invented SIM2 peptide for cancer therapeutic (Mahmud et al., Nature Communications, 2023)

• Evaluation of chemotherapeutic agent in luminal and her2 type breast cancers (Waddell & Mahmud et al., Cancers, 2021, PMID: 34199844)

• Learned and reported gene expression data analysis pipeline (Mahmud & Liao, Genomics data, 2015, PMID: 26217556) 

• Developed skills on cell and molecular biology techniques such as: 

• Cell culture, RNA/DNA isolation and quality evaluation

• PCR and Real-time PCR (TaqMan, SybrGreen I, and Roche UPL technologies)

• Protein isolation; Western blotting; Gel electrophoresis

• Expression cloning and Northern blotting

• Cell culture; Sanger sequencing and sequence analysis; ELISA

• Cell proliferation, invasion, and migration assays; 3D colony formation assay 

• Immunoprecipitation, ChIP qPCR; primer design; shRNA; CRISPR

• Flow cytometry for cell death analysis; Microscope/Fluorescent microscope; Nano-drop; Qubit; Agilent Bio-analyzer; Metal sonication. 

• Developed skills on in vivo animal studies

• Highly skilled in mouse breeding, direct tumor cell injection into mouse mammary fat pad and subcutaneous areas 

• Skilled in xenografting and mouse drug treatment or other intervention study. 

• Skilled in multi-organ collection including direct blood draw by cardiac puncture, gonadal fat, white fat, muscle, bone marrow, mesenteric lymph node, brain, pancreas, spleen, liver, intestine, etc. 

• Developed skills on computational biology and bioinformatics research

• Skilled with next-generation sequencing and sequencing data such as RNA-seq, ATCA-seq, ChIP-seq, and Single-cell sequencing data collection, mining, and analysis from TCGA, CCLE, CPTAC, Metabolomics Workbench, and NCBI GEO (Mahmud et al., Nature Comm, 2023; Mahmud et al., Nucleic acid Res, 2019; Mahmud et al., Genomics data, 2015; Ahmed et al., Cancers, 2023; Vijayakurup et al., JCI Insights, 2022; Waddell & Mahmud et al., Cancers, 2021; Masannat et al., Scientific reports, 2018)