PhD research assistant (August 2014 – August 2018)
Department of Anatomy and Cell Biology, University of Florida, FL 32603.
Advisor: Daiqing Liao, Ph.D. (https://physiology.med.ufl.edu/profile/liao-daiqing/)
• From 2014 to 2018: My research efforts contributed to 6 peer-reviewed manuscripts where 4 were first authored.
• Designed, conducted, and significantly contributed to multiomics projects to discover biomarkers, signaling mechanism, and novel therapeutics. Examples include:
Skilled with High-Pressure Liquid Chromatography coupled High Resolution Mass Spectrometry (UHPLC-HRMS)- based metabolomics/lipidomics (Mahmud et al., Analytical & Bioanalytical Chemistry, 2017, PMID: 28844081)
Discovered novel role of DAXX in de novo lipogenesis of triple negative breast and other cancer (Mahmud et al., Nature Communications, 2023)
Discovered DAXX as an oncogene in 18 human cancers (Mahmud et al., Nucleic acid Res, 2019)
Invented SIM2 peptide for cancer therapeutic (Mahmud et al., Nature Communications, 2023)
Evaluation of chemotherapeutic agent in luminal and her2 type breast cancers (Waddell & Mahmud et al., Cancers, 2021, PMID: 34199844)
Learned and reported gene expression data analysis pipeline (Mahmud & Liao, Genomics data, 2015, PMID: 26217556)
• Developed skills on cell and molecular biology techniques such as:
Cell culture, RNA/DNA isolation and quality evaluation
PCR and Real-time PCR (TaqMan, SybrGreen I, and Roche UPL technologies)
Protein isolation; Western blotting; Gel electrophoresis
Expression cloning and Northern blotting
Cell culture; Sanger sequencing and sequence analysis; ELISA
Cell proliferation, invasion, and migration assays; 3D colony formation assay
Immunoprecipitation, ChIP qPCR; primer design; shRNA; CRISPR
Flow cytometry for cell death analysis; Microscope/Fluorescent microscope; Nano-drop; Qubit; Agilent Bio-analyzer; Metal sonication.
• Developed skills on in vivo animal studies
Highly skilled in mouse breeding, direct tumor cell injection into mouse mammary fat pad and subcutaneous areas
Skilled in xenografting and mouse drug treatment or other intervention study.
Skilled in multi-organ collection including direct blood draw by cardiac puncture, gonadal fat, white fat, muscle, bone marrow, mesenteric lymph node, brain, pancreas, spleen, liver, intestine, etc.
• Developed skills on computational biology and bioinformatics research
Skilled with next-generation sequencing and sequencing data such as RNA-seq, ATCA-seq, ChIP-seq, and Single-cell sequencing data collection, mining, and analysis from TCGA, CCLE, CPTAC, Metabolomics Workbench, and NCBI GEO (Mahmud et al., Nature Comm, 2023; Mahmud et al., Nucleic acid Res, 2019; Mahmud et al., Genomics data, 2015; Ahmed et al., Cancers, 2023; Vijayakurup et al., JCI Insights, 2022; Waddell & Mahmud et al., Cancers, 2021; Masannat et al., Scientific reports, 2018)