Tomato crop is affected by viral pathogens worldwide. In India, the predominant virus affecting tomato is the Tomato leaf curl New Delhi virus (ToLCNDV). Several investigators have reported virus resistance in tomato using various strategies. We have used a novel strategy, artificial tasiRNA, to generate transgenic resistance in tomato against ToLCNDV (Singh et al., 2014). We also revealed the existence of a new virus-inducible tasiRNA locus in the genome of tomato (Singh et al., 2016).