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Our research primarily focuses on elucidating the impact of food-associated microbes on host metabolism and intestinal microbiota in the context of health and disease, with particular attention to obesity, metabolic disorders, and inflammation. A key aspect of our work is investigating the functional mechanisms of lactic acid bacteria in fermented foods and their potential health benefits. To further explore the efficacy of probiotics and other bioactive microbes, our research employs C. elegans as a model organism, providing a fundamental understanding of microbe-host interactions. Our expertise encompasses the cultivation of aerobic and anaerobic bacteria, DNA-based genotyping, immunological assays, next-generation sequencing, and animal models, integrating multidisciplinary approaches to advance knowledge in microbiome research and its applications in health sciences.
Our research primarily focuses on elucidating the impact of food-associated microbes on host metabolism and intestinal microbiota in the context of health and disease, with particular attention to obesity, metabolic disorders, and inflammation. A key aspect of our work is investigating the functional mechanisms of lactic acid bacteria in fermented foods and their potential health benefits. To further explore the efficacy of probiotics and other bioactive microbes, our research employs C. elegans as a model organism, providing a fundamental understanding of microbe-host interactions. Our expertise encompasses the cultivation of aerobic and anaerobic bacteria, DNA-based genotyping, immunological assays, next-generation sequencing, and animal models, integrating multidisciplinary approaches to advance knowledge in microbiome research and its applications in health sciences.
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最新研究成果發表：微生物與代謝體學揭示 Debaryomyces hansenii 提升乾式熟成黑豬肉風味之機制
恭喜本實驗室成員！我們的最新研究成果已正式發表於國際知名期刊 《Food Microbiology》。
本研究透過多種組學技術，深入探討黑豬肉於低溫乾式熟成過程中的微生物群落動態與代謝物變化 。我們發現漢遜德巴利酵母（Debaryomyces hansenii）是熟成後期的優勢菌種，且其豐度與多種關鍵風味代謝物（如自由氨基酸）呈顯著正相關 。透過實際接種分離菌株進行驗證，證實 D. hansenii 能顯著加速蛋白質分解並強化肉品的鮮味與香氣 。此成果為開發新型乾式熟成策略與高價值肉製品提供了重要科學依據 。
New Research Publication: Microbial and Metabolomic Analyses Elucidate the Debaryomyces hansenii-Mediated Flavor Enhancement in Dry-Aged Black Pig Pork.
This study integrated microbial and metabolomic analyses to elucidate the key drivers of flavor development in dry-aged black pig pork. Our findings identify Debaryomyces hansenii as a naturally dominant fungal species that strongly correlates with flavor-active metabolites, particularly free amino acids. Validation experiments confirmed that targeted inoculation with D. hansenii starter cultures significantly enhances proteolysis, umami intensity, and overall meat quality. These results support the development of innovative microbial-assisted aging strategies for premium meat products.
Article Title: Microbial and metabolomic analyses elucidate the Debaryomyces hansenii-mediated flavor enhancement in dry-aged black pig pork
Full Access: https://doi.org/10.1016/j.fm.2026.105088
2026.03.27
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New Publication: Anti-Obesity Mechanisms of Fermented Purple Sweet Potato via Lipidomic Modulation
We are pleased to announce that our lab's latest research has been published in the International Journal of Molecular Sciences (IJMS).
This study explores how Fermented Purple Sweet Potato (Tainung No. 73) Extract (FSPE) combats high-fat diet-induced obesity. Our findings highlight that fermentation with specific lactic acid bacteria significantly boosts Indolelactic acid (ILA) levels. Through targeted lipidomics, we demonstrated that FSPE effectively modulates ceramides and acylcarnitines, promotes gut short-chain fatty acids (SCFAs), and reduces inflammation, leading to reduced adiposity and improved hepatic steatosis.
Article Title: Targeted Lipidomics and Lipid Metabolism Elucidate Anti-Obesity Effects of Lactic Acid Bacteria-Fermented Purple Sweet Potato Tainung No. 73 Extract in Obese Mice
Full Access: https://doi.org/10.3390/ijms27031489
本實驗室最新發表：乳酸菌發酵紫地瓜透過脂質組學調控達成抗肥胖效果
恭喜本實驗室成員！我們的最新研究成果已正式發表於國際知名期刊 《International Journal of Molecular Sciences》(IJMS)。
本研究深入探討了發酵台農73號紫地瓜 (FSPE) 對抗高脂飲食誘導肥胖的作用機制。研究發現，透過特定乳酸菌發酵，能顯著提升紫地瓜中 吲哚乳酸 (Indolelactic acid) 的含量。藉由標靶脂質組學分析證實，FSPE 能有效調控小鼠體內的神經醯胺 (Ceramides) 與醯基肉鹼 (Acylcarnitines) 代謝，並透過增加腸道短鏈脂肪酸與降低發炎，達到減輕體重、縮小脂肪細胞及改善脂肪肝之效益。
2026.03.02
PREVIOUS RESEARCHES
PREVIOUS RESEARCHES
Lactobacillus rhamnosus LRH05 (LRH05) and Lactobacillus reuteri LR47 (LR47) are screened out with lower triglyceride expression in vitro. The mice are fed a control diet (CD), HFD, or HFD supplemented with a dose of LRH05 or LR47 at 10^9 CFU per mouse per day for 10 weeks, respectively. The results demonstrate that LRH05, but not LR47, significantly reduce body weight gain and the weight of WAT, as well as improve hepatic steatosis and glucose intolerance. LRH05 regulates the Mogat1, Igf-1, Mcp-1, and F4/80 mRNA expression and decreases macrophage infiltration in WAT. LRH05 shows an increase in butyric and propionic acid-producing bacteria, including Lachnoclostridium, Romboutsia, and Fusobacterium that is coincident with the increased fecal propionic acid and butyric acid levels. LRH05 shows a strain-specific effect on ameliorating the pro-inflammatory process by reducing inflammatory macrophage infiltration and the expression of inflammation-related genes in mice. Thus, LRH05 can be considered a potential probiotic strain to prevent obesity.
Lactobacillus rhamnosus LRH05 (LRH05) and Lactobacillus reuteri LR47 (LR47) are screened out with lower triglyceride expression in vitro. The mice are fed a control diet (CD), HFD, or HFD supplemented with a dose of LRH05 or LR47 at 10^9 CFU per mouse per day for 10 weeks, respectively. The results demonstrate that LRH05, but not LR47, significantly reduce body weight gain and the weight of WAT, as well as improve hepatic steatosis and glucose intolerance. LRH05 regulates the Mogat1, Igf-1, Mcp-1, and F4/80 mRNA expression and decreases macrophage infiltration in WAT. LRH05 shows an increase in butyric and propionic acid-producing bacteria, including Lachnoclostridium, Romboutsia, and Fusobacterium that is coincident with the increased fecal propionic acid and butyric acid levels. LRH05 shows a strain-specific effect on ameliorating the pro-inflammatory process by reducing inflammatory macrophage infiltration and the expression of inflammation-related genes in mice. Thus, LRH05 can be considered a potential probiotic strain to prevent obesity.
Ref: Mol. Nutr. Food Res.,2021
DOI : 10.1002/mnfr.202100348
We demonstrated that the specific bacterial strains isolated from kefir, Lactobacillus kefirnofaciens M1 and Lactobacillus mali APS1, possess obesity and anti-obesity effects, respectively, in high-fat diet. We systematically investigated whether APS1 and M1 affect energy homeostasis and lipid metabolism in HFD-induced obese mice and how this might be achieved. We observed that the M1/APS1 intervention influenced fat accumulation by regulating adipogenesis and inflammation-related marker expression both in vitro and in a HFD-induced obesity mice model. We also observed putative links between key taxa and possible metabolic processes of the gut microbiota. Notably, families Christensenellaceae and S24_7 were negatively correlated with body weight gain through increase in the essential esterized carnitine for energy expenditure. These results suggest the importance of specific probiotic interventions affecting leanness and obesity of subjects under a HFD, which are operated by modulating the tripartite relationship among the host, microbiota, and metabolites.
We demonstrated that the specific bacterial strains isolated from kefir, Lactobacillus kefirnofaciens M1 and Lactobacillus mali APS1, possess obesity and anti-obesity effects, respectively, in high-fat diet. We systematically investigated whether APS1 and M1 affect energy homeostasis and lipid metabolism in HFD-induced obese mice and how this might be achieved. We observed that the M1/APS1 intervention influenced fat accumulation by regulating adipogenesis and inflammation-related marker expression both in vitro and in a HFD-induced obesity mice model. We also observed putative links between key taxa and possible metabolic processes of the gut microbiota. Notably, families Christensenellaceae and S24_7 were negatively correlated with body weight gain through increase in the essential esterized carnitine for energy expenditure. These results suggest the importance of specific probiotic interventions affecting leanness and obesity of subjects under a HFD, which are operated by modulating the tripartite relationship among the host, microbiota, and metabolites.
Ref: Front. Microbiol., 2020 https://doi.org/10.3389/fmicb.2020.01454
鼠李糖乳桿菌LRH05分離株及其用途
LACTOBACILLUS RHAMNOSUS LRH05 ISOLATE AND USES OF THE SAME
Patent Number: I776375
Issued Date: 2022/09/01
Inventor:
陳詠宗 (中華民國); CHEN, YUNG TSUNG (TW);
林金生 (中華民國); LIN, JIN-SENG (TW)
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