RESEARCH INTERESTS
RESEARCH OVERVIEW
Chen's main research interests are to investigate the effect of food microbes on host metabolism and the intestinal microbe in health and disease, focusing on obesity, metabolic disorder, and inflammation. Research spans the cultivation of aerobic and anaerobic bacteria, DNA-based genotyping, immune technology, next-generation sequencing, and animal model.
RESEARCH PROJECTS
2022-2023
Industry-Academy Cooperation Project
開發一潛力功效菌種於豆渣開發為功能性食品原料之應用
Development and Application of Okara Fermentative Products as Food Material
2023-2024
NSTC Project
探討甘藷水萃物及巴氏滅菌艾克曼嗜黏蛋白菌協同作用對高脂飼料誘導非酒精性脂肪肝之機理研究
The mechanistic study of the synergistic effect of sweet potato water extract and pasteurized Akkermansia muciniphila on high-fat diet-induced non-alcoholic fatty liver disease
COA Science and Technology Program
微生物體學與代謝體學技術應用於低溫乾式熟成黑豬肉品開發及品質鑑定分析
Application of Microbiome and Metabolomics Technology in the Development of Low-temperature Dryaging Black Pig Meat and Meat Quality Analysis
COA Cooperation Project
石斑魚加工肉品技術提升之研究
The Improvement of Grouper Meat Processing Technology
PREVIOUS RESEARCHES
Lactobacillus rhamnosus LRH05 (LRH05) and Lactobacillus reuteri LR47 (LR47) are screened out with lower triglyceride expression in vitro. The mice are fed a control diet (CD), HFD, or HFD supplemented with a dose of LRH05 or LR47 at 10^9 CFU per mouse per day for 10 weeks, respectively. The results demonstrate that LRH05, but not LR47, significantly reduce body weight gain and the weight of WAT, as well as improve hepatic steatosis and glucose intolerance. LRH05 regulates the Mogat1, Igf-1, Mcp-1, and F4/80 mRNA expression and decreases macrophage infiltration in WAT. LRH05 shows an increase in butyric and propionic acid-producing bacteria, including Lachnoclostridium, Romboutsia, and Fusobacterium that is coincident with the increased fecal propionic acid and butyric acid levels. LRH05 shows a strain-specific effect on ameliorating the pro-inflammatory process by reducing inflammatory macrophage infiltration and the expression of inflammation-related genes in mice. Thus, LRH05 can be considered a potential probiotic strain to prevent obesity.
Ref: Mol. Nutr. Food Res.,2021
DOI : 10.1002/mnfr.202100348
We demonstrated that the specific bacterial strains isolated from kefir, Lactobacillus kefirnofaciens M1 and Lactobacillus mali APS1, possess obesity and anti-obesity effects, respectively, in high-fat diet. We systematically investigated whether APS1 and M1 affect energy homeostasis and lipid metabolism in HFD-induced obese mice and how this might be achieved. We observed that the M1/APS1 intervention influenced fat accumulation by regulating adipogenesis and inflammation-related marker expression both in vitro and in a HFD-induced obesity mice model. We also observed putative links between key taxa and possible metabolic processes of the gut microbiota. Notably, families Christensenellaceae and S24_7 were negatively correlated with body weight gain through increase in the essential esterized carnitine for energy expenditure. These results suggest the importance of specific probiotic interventions affecting leanness and obesity of subjects under a HFD, which are operated by modulating the tripartite relationship among the host, microbiota, and metabolites.
Ref: Front. Microbiol., 2020 https://doi.org/10.3389/fmicb.2020.01454
鼠李糖乳桿菌LRH05分離株及其用途
LACTOBACILLUS RHAMNOSUS LRH05 ISOLATE AND USES OF THE SAME
Patent Number: I776375
Issued Date: 2022/09/01
Inventor:
陳詠宗 (中華民國); CHEN, YUNG TSUNG (TW);
林金生 (中華民國); LIN, JIN-SENG (TW)