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Role of HAUS7 as a DOCK3 binding partner in facilitating axon regeneration
Role of HAUS7 as a DOCK3 binding partner in facilitating axon regeneration
#Kiyota N, #Shinozaki Y, Guo X, Kimura A, Kawamura K, Nishijima E, Honda S, Harada C, Nakazawa T, Namekata K, Harada T (#co-first)
Science Advances, 2025, 11, eadq7105.
【解説】これまで我々は、視神経再生を目的として網膜神経節細胞の軸索伸長因子に関する研究を進めてきました。その過程でDOCK3という分子を発見しましたが、今回はその新たな結合因子としてAugmin family memberの１つHAUS7を同定しました。HAUS7はDOCK3依存的に軸索先端に輸送され、BDNF/TrkBシグナルによりDOCK3がリン酸化されると解離します。HAUS7欠損やノックダウンは視神経傷害後の再生を著しく阻害しました。また、公共データを用いた解析からは、緑内障においてHaus7発現が低下することや、視神経傷害後の再生を促進した網膜神経節細胞で発現が増加するなど、病態や再生との関連が明らかとなりました。
Astrocytic dysfunction induced by ABCA1 deficiency causes optic neuropathy
Astrocytic dysfunction induced by ABCA1 deficiency causes optic neuropathy
Shinozaki Y, Leung A, Namekata K, Saitoh S, Nguyen HB, Takeda A, Danjo Y, Morizawa Y, Shigetomi E, Sano F, Yoshioka N, Takebayashi H, Ohno N, Segawa T, Miyake K, Kashiwagi K, Harada T, Ohnuma S, Koizumi S.
Science Advances, 2022, 8, abq1081.
【解説】「緑内障」は、日本で最大の失明原因となる病気です。これまで、緑内障は主に眼圧が原因と考えられていましたが、実際は日本の患者の多くは正常な眼圧を示すため、眼圧以外の要因に関する研究が必要でした。最新の研究から、グリア細胞の異常が緑内障の原因となることが明らかとなりました。脳や網膜に存在する「グリア」と呼ばれる非神経細胞のうち、特に「アストロサイト」という細胞でコレステロール輸送に関わる分子であるABCA1が不足すると、炎症の惹起と神経の過度な興奮が緑内障の発症につながることが明らかになりました。
Glaucoma is the leading cause of blindness in Japan. While elevated intraocular pressure (IOP) has been thought to be the main cause, most Japanese glaucoma patients have normal IOP levels. This led us to explore other factors contributing to the disease. Our recent study revealed that dysfunction in glial cells is responsible for normal-tension glaucoma. Glia are non-neuronal cells found in the nervous system, such as the brain and retina. Specifically, astrocytes, a type of glial cell, can trigger normal-tension glaucoma by causing inflammation and excessive nerve activity when the cholesterol exporter ABCA1 is absent in the cell.
Loss of P2Y1 receptors triggers glaucoma-like pathology in mice
Loss of P2Y1 receptors triggers glaucoma-like pathology in mice
#Hamada K, #Shinozaki Y, Namekata K, Matsumoto M, Ohno N, Segawa T, Kashiwagi K, Harada T, Koizumi S. (#co- first authors)
British Journal of Pharmacology, 2021, 178: 4552-4578.
In this study, we found that topical application of P2Y1 receptor agonist transiently reduces intraocular pressure in mice. P2Y1 receptors were expressed in the ciliary body and trabecular meshwork which are essential for aqueous production/draining. Loss of P2Y1R caused chronic ocular hypertension and age-associated glaucoma like phenotypes.
Purinergic dysregulation causes hypertensive glaucoma-like optic neuropathy
Purinergic dysregulation causes hypertensive glaucoma-like optic neuropathy
Shinozaki Y, Kashiwagi K, Namekata K, Takeda A, Ohno N, Robaye B, Harada T, Iwata T, Koizumi S.
JCI Insight, 2017, 2: e93456.
We found that UDP, an endogenous agonist for P2Y6 receptor, reduced intraocular pressure. P2Y6 receptors were expressed in the non-pigmented epithelium of the ciliary body which regulates the production of aqueous humor. Lack of P2Y6 receptor showed chronic ocular hypertension, age-associated RGC degeneration and visual impairment.
Transformation of Astrocytes to a Neuroprotective Phenotype by Microglia via P2Y1 Receptor Downregulation
Shinozaki Y, Shibata K, Yoshida K, Shigetomi E, Gachet C, Ikenaka K, Tanaka KF, Koizumi S.
Cell Report, 2017, 19: 1151-1164.
Microglia are highly sensitive to brain environment. We found that microglia become reactive at early stages after traumatic brain injury and regulate astrocytic responses. Microglia-derived cytokines down-regulated astrocytic P2Y1R thereby enhancing their reactivity and protective responses.
Direct Observation of ATP-induced Conformational Changes in Single P2X4 Receptors
Direct Observation of ATP-induced Conformational Changes in Single P2X4 Receptors
*Shinozaki Y, Sumitomo K, Tsuda M, Koizumi S, Inoue K, Torimitsu K. (*Corresponding Author)
PLoS Biology, 2009, 7: e1000103.
In this study, we used atomic force microscopy (AFM) to visualize single P2X4 receptor complex. AFM enables us to directly visualize surface structure of various biological molecules including nucleic acids, lipids and proteins. We purified P2X4 receptor complex and deposited it on mica or reconstituted into supported lipid bilayers. ATP stimulation evoked relatively slow structural changes known as "pore-dilation". Our results provided the evidence showing P2X4R exhibit pore dilation without adapter proteins such as connexin or pannexin.
Full Publication
Full Publication
2025
[89] #Kiyota N, #Shinozaki Y, Guo X, Kimura A, Kawamura K, Nishijima E, Honda S, Harada C, Nakazawa T, Namekata K, Harada T. (#co-first) Role of HAUS7 as a DOCK3 binding partner in facilitating axon regeneration
Sci Adv, 2025, 11, eadq7105. doi: 10.1126/sciadv.adq7105
[88] 篠崎陽一、行方和彦、原田高幸. シナプス障害を基盤とした緑内障発症機構と治療戦略. 医学のあゆみ295(2), 128-134, 2025
[87] 清田直樹、篠崎陽一、郭 暁麗、木村敦子、川村和人、西島義道、本田紗里、原田知加子、中澤 徹、行方和彦、原田高幸. HAUS7はDOCK3の結合パートナーであり、視神経再生を促進する. 日本眼科学会雑誌129(11), 1110, 2025.
[86] 野呂隆彦、郭 暁麗、菊池理加、佐藤賢哉、行方和彦、篠崎陽一、原田知加子、圦本晃海、橋本菜名子、守屋恵子、中野 匡、佐々木えりか、原田高幸. コモン・マーモセットを用いた網膜加齢変化の解析. 日本眼科学会雑誌129(11), 1109, 2025.
[85] 篠崎陽一, 緑内障とグリア細胞. 実験医学 2025, 43(10), 141-149.
[84] Ono Y, Tanaka K, Sato E, Ito M, Zhang D, Honda M, Ogawa T, Tagaya H, Fukuda T, Shinozaki Y, Kato K, Koizumi S, Yoshino O. Activated eosinophil plays a role in promoting fibrosis in endometriotic lesion. Sci Rep 2025, 15, 28015. DOI: 10.1038/s41598-025-13855-x
[83] Ishimori T, Saito M, Yuki M, Hattori M, Shuzui M, Nagoshi S, Kono S, Isago H, Tamiya H, Miyashita N, Takashi Ishii T, Mikami Y, Nagase T, Terasaki Y, Shinozaki Y, Mitani A. Sirtuin 3 deficiency exacerbates emphysema and lung inflammation in a murine model of chronic obstructive pulmonary disease. AJP Lung Cell Mol Physiol 2025, 329, 200-203. DOI: 10.1152/ajplung.00212.2024
[82] Noro T, Guo X, Kikuchi R, Sato K, Namekata K, Shinozaki Y, Harada C, Yurimoto T, Hashimoto K, Moriya-Ito K, Nakano T, Sasaki E, Harada T.
Age-related decline in retinal function in mormosets.
Sci Rep, 2025, 15, 22374. doi.org/10.1038/s41598-025-05262-z
[81] Noro T, Guo X, Namekata K, Shinozaki Y, Hashimoto N, Moriya-Ito K, Harada C, Nakano T, Harada T.
Valproic acid prevents NMDA-induced retinal degeneration in marmosets.
Neurosci Lett, 2025, 855, 138197. doi.org/10.1016/j.neulet.2025.138197
[80] 篠崎陽一、中村信介
序文：特集 「見る」を科学する～眼科疾患創薬研究の新展開～
2024
[79] Shinozaki Y
Glial interactions in glaucoma [Editorial]
Front in Ophthalmol 2024, 4, 139355. doi.org/10.3389/fopht.2024.1393555
[78] Shigetomi E, Suzuki H, Hirayama YJ, Sano F, Nagai Y, Yoshihara K, Koga K, Tateoka T, Yoshioka H, Shinozaki Y, Kinouchi, H, Tanaka KF, Bito H, Tsuda M, Koizumi S.
Disease-relevant upregulation of P2Y1 receptor in astrocytes enhances neuronal excitability via IGFBP2.
Nat Commun, 2024, 15, 6525. doi: 10.1038/s41467-024-50190-7.
[77] Kubota Y, Shigetomi E, Saito K, Shinozaki Y, Kobayashi K, Tanaka M, Parajuli B, Tanaka KF, Koizumi S.
Establishment and Use of Primary Cultured Astrocytes from Alexander Disease Model Mice. IJMS 2024, 25, 12100.
[76] Sotozono A, Namekata K*, Guo X, Shinozaki Y, Harada C, Noro T, Nakano T, Harada T
Membrane-anchored intracellular insulin receptor or insulin-like growth factor-1 receptor elicits ligand-independent downstream signaling
Biochem Biophys Rep, 2024, 39, 101799.
2023
[75] Shinozaki Y*, Namekata K, Guo X, Harada T
Glial cells as a promising therapeutic target of glaucoma: beyond the IOP
Front Ophthalmol (Glaucom), 2023, 3:1310226. (*Corresponding author)
[74] Namekata K, Noro T, Nishijima E, Sotozono A, Guo X, Harada C, Shinozaki Y, Mitamura Y, Nakano T, Harada T
Drug combination of topical ripasudil and brimonidine enhances neuroprotection in a mouse model of optic nerve injury.
J Pharmacol Sci, 2024,154, 326-333.
[73] Le Y-P, Saito K, Parajuli B, Sakai K, Kubota Y, MIyakawa M, Shinozaki Y, Shigetomi E, Koizumi S.
Severity of peripheral infection differentially affects brain functions in mice via microglia-dependent and -independent mechanisms. IJMS, 2023, 24 (24), 17597. (IF = 6.1)
[72] 篠崎陽一
神経科学トピックス：グリアの異常が目の病気の原因となる
神経科学ニュース 3, 47, 2023
[71] 篠崎陽一
緑内障：緑内障とグリア機能
[70] 篠﨑陽一，Alex Leung，行方和彦，齋藤成，Huy Bang Nguyen，武田明子，檀上洋右，森澤陽介，繁冨英治， 佐野史和，吉岡望，竹林浩秀，大野伸彦，瀬川高弘，三宅邦夫，柏木賢治，原田高幸，大沼信一，小泉修一.
アストロサイトのABCA1 欠損は視神経症を誘発する
日本眼科学会雑誌127(5), 582-583, 2023.
[69] 篠崎陽一、小泉修一
アストロサイトの機能異常による正常眼圧緑内障発症機構
[68] Saito K, Shigetomi E, Shinozaki Y, Kobayashi K, Parajuli B, Kubota Y, Sakai K, Miyakawa M, Horiuchi H, Nabekura J, Koizumi S.
Microglia sense astrocyte dysfunction and prevent disease progression in an Alexander disease model.
Brain, 2023, awad358 (IF = 14.5) Press Release (in Japanese)
[67] Namekata K, Tsuji N, Guo X, Nishijima E, Honda S, Kitamura Y, Yamasaki A, Kishida M, Takeyama J, Ishikawa H, Shinozaki Y, Kimura A, Harada A, Harada T. Neuroprotection and axon regeneration by novel low-molecular-weight compounds through the modification of DOCK3 conformation.
Cell Death Discovery , 2023, 9: 166 (IF = 7.1)
[66] Ihara T, Shinozaki Y, Shigetomi E, Danjo Y, Tsuchiya S, Kanda M, Kamiyama M, Takeda M, Koizumi S, Mitsui T.
G protein-coupled receptor 55 activated by palmitoylethanolamide is associated with the development of nocturia associated with circadian rhythm disorders.
Life Sci 2023, 332, 122072-122072.
[65] Ono Y, Yoshino O, Ito M, Tanaka K, Tokura Y, Ogawa Y, Tagaya H, Ogi M, Shinozaki Y, Koizumi S, Hirata S. Endometriotic cell-derived adenosine triphosphate (ATP) promotes the fibrosis of endometriotic lesions via eosinophil activation. J Reprod Immunol. 2023, 156: 103885.
[64] Shinozaki Y, Kashiwagi K, Koizumi S
Astrocyte Immune Functions and Glaucoma (Review)
Int J Mol Sci, 2023, 24(3), 2747 (IF = 6.1)
[63] Shinozaki Y, Saito K, Kashiwagi K, Koizumi S.
Ocular P2 receptors and glaucoma. (Invited Review)
Neuropharmacology, 2023, 222: 109302. (IF = 5.3)
2022
[62] Shinozaki Y, Leung A, Namekata K, Saitoh S, Nguyen HB, Takeda A, Danjo Y, Morizawa MY, Shigetomi E, Sano F, Yoshioka N, Takebayashi H, Ohno N, Segawa T, Miyake K, Kashiwagi K, Harada T, Ohnuma SI, Koizumi S.
Astrocytic dysfunction induced by ABCA1 deficiency causes optic neuropathy.
Science Advances, 2022, 8, abq1081. (IF = 15.0)
Press Release (UY, TMiMS, NIPS, Niigata Univ, JST-China)
Web News (日本経済新聞Web, CB News, Yahoo, 医療NEWS (QLifePro), iptops.com, 信濃毎日新聞, NEWS Twit, Sawai medical site, 日本の研究.com)
[61] 篠崎陽一、Parajuli Bijay
網膜ミクログリアと緑内障 -非自律性神経変性機構と治療標的の可能性-
[60] Parajuli B, Shinozaki Y, Shigetomi E, Koizumi S. (2022) Transplantation of Human Induced Pluripotent Stem Cell-derived Microglia in Immunocompetent Mice Brain via Non-invasive Transnasal Route.
[59] Danjo Y, Shigetomi E, Hirayama Y, Kobayashi K, Ishikawa T, Fukazawa Y, Shibata K, Takanashi K, Parajuli B, Shinozaki Y, Kim SK, Nabekura J, Koizumi S.
Transient astrocytic mGluR5 expression drives synaptic plasticity and subsequent chronic pain in mice.
JEM, 2022, 219: e20210989. (Press Release) (IF = 15.3)
[58] Parajuli B, Shinozaki Y, Shigetomi E, Koizumi S.
Transplantation of Human Induced Pluripotent Stem Cell-derived Microglia in Immunocompetent Mice Brain via Non-invasive Transnasal Route. J. Vis. Exp., 2022, 183, e63574.
[57] Danjo Y#, Shinozaki Y#, Natsubori A, Kashiwagi K, Kanji F Tanaka, Koizumi S.
The Mlc1 promoter directs Muller cell-specific gene expression in the retina.
TVST ,2022, 11: 25. (IF = 3.1)
[56] Ihata T, Shimura H, Tsuchiya S, Kanda M, Kira S, Sawada N, Takeda M, Mitsui T, Shigetomi E, Shinozaki Y, Koizumi S
Sci Rep, 2022, 12: 3050.
2021
[55] Hamada K#, Shinozaki Y#, Namekata K, Matsumoto M, Ohno N, Segawa T, Kashiwagi K, Harada T, Koizumi S.
Loss of P2Y1 receptor triggers glaucoma-like pathology in mice.
Br J Pharmacol. 2021, 178: 4552-4571. (Press Release) (IF = 9.5)
[54] Parajuli B, Saito H, Shinozaki Y, Miwa H, Yoneda S, Omachi S, Asaki T, Takahashi K, Fujita M, Nakashima K, Koizumi S.
Transnasal transplantation of human induced pluripotent stem cell-derived microglia to the brain of immunocompetent mice.
GLIA, 2021, 69: 2332-2348. (IF = 8.1)
[53] Tanaka M, Parajuli B, Shigetomi E, Nagatomo H, Shinozaki Y, Hirayama Y, Saito K, Kubota Y, Danjo Y, Lee JH, Kim SK, Nabekura J, Koizumi S.
Adenosine A2B receptor down-regulates metabotropic glutamate receptor 5 in astrocytes during postnatal development.
GLIA, 2021, 69: 2546-2558. (IF = 8.1)
[52] Sano F, Shigetomi E, Shinozaki Y, Tsuzukiyama H, Saito K, Mikoshiba K, Sugita K, Aihara M, Koizumi S.
Reactive astrocyte-driven epileptogenesis is induced by microglia initially activated following status epilepticus
JCI Insight, 2021, 6: e135391.
[51] Doi H, Parajuli B, Horio T, Shigetomi E, Shinozaki Y, Noda T, Takahashi K, Hattori T, Koizumi S, Sawada T.
Development of a label-free ATP imaging sensor for analyzing spatiotemporal patterns of ATP release from biological tissues.
Sens Act B: Chemical, 2021, 335, 129686 .
[50] Shinozaki Y, Koizumi S.
Potential role of astrocytes and Muller cells in the pathogenesis of glaucoma (Review)
J Pharmacol Sci, 2021, 145: 262-267.
2020
[49] Shinozaki Y, Koizumi S.
Potential role of glial dysfunction in pathogenesis of glaucoma (Japanese)
Folia Pharmacol. Jpn. 2020, 155:87-92.
2019
[48] Ihara T, Nakamura Y, Mitsui T, Tsuchiya S, Kanda M, Kira S, Nakagomi H, Sawada N, Kamiyama M, Shigetomi E, Shinozaki Y, Yoshiyama M, Nakao A, Koizumi S, Takeda M.
Intermitttent restraint stress induces circadian misalignment in the mouse bladder, leading to nocturia.
[47] Shinozaki Y, Danjo Y, Koizumi S
Microglial ROCK is essential for chronic methylmercury-induced neurodegeneration
Journal of Neurochemistry, 2019, 151: 64-78.
2018
[46] Hoshi T, Toyama T, Shinozaki Y, Koizumi S, Lee JY, Naganuma A and Hwang GW
Evaluation of M1-microglial activation by neurotoxic metals using optimized organotypic cerebral slice cultures.
J. Toxicol. Sci. 2019, 44: 471-479.
[45] Kinoshita M, Hirayama Y, Fujishita K, Shibata K, Shinozaki Y, Shigetomi E, Takeda A, Ngoc Le HP, Hayashi H, Hiasa M, Moriyama Y, Ikenaka K, Tanaka KF, Koizumi S.
Anti-depressesant fluoxetine reveals its therapeutic effect via astrocytes.
EBioMed 2018, 32: 72-83
[44] #Takeda A, #Shinozaki Y, Kashiwagi K, Ohno N, Eto K, Wake H, Nabekura J and Koizumi S. Microglia mediate non-cell-autonomous cell death of retinal ganglion cells
GLIA 2018, 66: 2366-2384. (#co-first authors)
[43] Ihara T, Mitsui T, Nakamura Y, Kanda M, Tsuchiya S, Kira S, Nakagomi H, Sawada N, Kamiyama M, Hirayama Y, Shigetomi E, Shinozaki Y, Yoshiyama M, Nakao A, Takeda M and Koizumi S.
The Oscillation of intracellular Ca2+ influx associated with the circadian expression of Piezo1 and TRPV4 in the bladder urothelium.
2017
[42] Shinozaki Y, Kashiwagi K, Namakata K, Takeda A, Ohno N, Robaye B, Harada T, Iwata T and Koizumi S.
Purinergic dysregulation causes hypertensive glaucoma-like optic neuropathy
JCI Insight 2017, 2(19): e93456. (Press Release)
[41] Ihara T, Mitsui T, Nakamura Y, Tsuchiya S, Kira S, Nakagomi H, Sawada N, Hirayama Y, Shibata K, Shigetomi E, Shinozaki Y, Yoshiyama M, Nakao A, Takeda M and Koizumi S.
The circadian expression of Piezo1, TRPV4, Coonexin26 and VNUT, associated with expression levels of the clock genes in mouse primary cultured urothelial cells.
Neurourology and Urodynamics,2018, 37: 942-951
[40] Katoh M, Wu B, Ngyen HB, Thai TQ, Yamasaki R, Lu H, Rietsh AM, Zorlu MM, Shinozaki Y, Saitoh Y, Saitoh S, Satoh K, Ikenaka K, Koizumi S, Ransohoff RM and Ohno N
Polymorphic regulation of mitochondrial fission and fusion modifies phenotypes of microglia in neuroinflammation.
[39] Shinozaki Y, Shibata K, Yoshida K, Shigetomi E, Gachet C, Ikenaka K, Tanaka KF and Koizumi S
Transformation of astrocytes to a neuroprotective phenotype by microglia via P2Y1 receptor downregulation.
Cell Rep 2017, 19: 1151-1164. (Press Release)
[38] Ihara T, Mitsui T, Nakamura Y, Kira S, Nakagomi H, Sawada N, Hirayama Y, Shibata K, Shigetomi E, Shinozaki Y, Yoshiyama M, Andersson KL, Nakao A, Takeda M and Koizumi S
Clock Genes Regulate the Circadian Expression of Piezo1, TRPV4, Connexin26 and VNUT in an Ex Vivo Mouse Bladder Mucosa.
PLoS One 2017, 12: e0168234
2016
[37] Nakagomi H, Yoshiyama M, Mochizuki T, Miyamoto T, Komatsu R, Imura Y, Morizawa Y, Hiasa M, Miyaji T, Kira S, Araki I, Fujishita K, Shibata K, Shigetomi E, Shinozaki Y, Ichikawa R, Uneyama H, Iwatsuki K, Nomura M, Groat WC, Moriyama Y, Takaeda M and Koizumi S
Urothelial ATP exocytosis: regulation of bladder compliance in the urine storage phase.
[36] Ihara T, Mitsui T, Kira S, Miyamoto T, Sawada N, Nakagomi H, Yoshiyama M, Takeda M, Nakamura Y, Nakao A, Shinozaki Y, Koizumi S
The Clock mutant mouse is a novel experimental model for nocturia and nocturnal polyuria
Neurourol Urodyn, 2016, 36: 1034-1038.
[35] Kim SK, Hayashi H, Ishikawa T, Shibata K, Shigetomi E, Shinozaki Y, Inada H, Roh SE, Kim SJ, Lee H, Bae H, Moorhouse AJ, Mikoshiba K, Fukazawa Y, Koizumi S* and Nabekura J*
Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain.
J Clin Invest, 2016, 126: 1983-1997. (Press Release)
[34] Taguchi M*, Shinozaki S*, Kashiwagi K, Shigetomi E, Robaye B, Koizumi S. (*co-first)
Muller cell-mediated neurite outgrowth of the retinal ganglion cells via P2Y6 receptor signals
J Neurochem, 2016, 136: 741-751.
2014
[33] Shinozaki Y, Nomura M, Iwatsuki K, Moriyama Y, Gachet C, Koizumi S
Microglia trigger astrocyte-mediated neuroprotection via purinergic gliotransmission
[32] Shinozaki Y*, Tanaka A, Kasai N, Torimitsu K, Sumitomo K.
Ligand-induced structural changes in membrane-reconstituted ion channel observed with atomic force microscopy.
Appl. Phys. Express. 2014, 7(2), 027001 *corresponding author
2013
[31] Imura Y, Morizawa Y, Komatsu R, Shibata K, Shinozaki Y, Kasai H, Moriishi K, Moriyama Y, Koizumi S.
Microglia release ATP by exocytosis.
[30] Noguchi Y#, Shinozaki Y#, Fujishita K#, Shibata K, Imura Y, Morizawa Y, Gachet C, Koizumi S. (#equal contribution)
Astrocytes protect neurons against methylmercury via ATP/P2Y1 receptor-mediated pathways in astrocytes
[29] Takata F, Dohgu S, Yamaguchi A, Matsumoto J, Machida T, Fujishita K, Shibata K, Shinozaki Y, Sato K, Kataoka Y, Koizumi S.
PLoS One 2013, 8, e55166
2012
[28] K. Sumitomo*, A. McAllister, Y. Tamba, Y. Kashimura, A. Tanaka, Y. Shinozaki, and K. Torimistsu K
Ca2+ ion transport through channels formed by alpha-hemolysin analyzed using a microwell array on a Si substrate
Biosens Bioelectron, 31, 445-450
2011
[27] Shinozaki Y*, Sumitomo K, Tanaka A, Kasai N, Torimitsu K.
Examination of Ion Channel Protein Orientation in Supported Lipid Bilayers
Appl. Phys. Express (APEX), 2011, 4, 107001.
[26] Shibata K, Sugawara T, Fujishita K, Shinozaki Y, Matsukawa T, Suzuki T, Koizumi S.
The astrocyte-targeted therapy by Bushi for the neuropathic pain in mice.
PLoS One, 2011, 6: e23510 (selected as the top 10% most cited PLoS One article)
[25] 篠崎陽一*、住友弘二、河西奈保子、田中あや、鳥光慶一
原子間力顕微鏡を用いた生体分子のトポロジー観察
2010
[24] K. Sumitomo*, Tamba Y, Shinozaki Y, Torimitsu K.
Confinement of fluorescent probes in micro-wells on Si substrates by sealing with lipid bilayer
Appl. Phys. Express (APEX), 2010, 3, 107001.
[23] Nakashima H*, Furukawa K, Kashimura Y, Sumitomo K, Shinozaki Y, Torimitsu K.
Pattern formationa and molecular transport of histidine-tagged GFPs using supported lipid bilayers.
Langmuir 2010, 26(15), 12716-12721.
[22] Shinozaki Y*, Sumitomo K, Furukawa K, Miyashita H, Tamba Y, Kasai N, Nakashima H, Torimitsu K.
Visualization of membrane protein suspended over nanoscale well.
Appl. Phys. Express (APEX), 2010, 3, 027002.
(APEX top pageに掲載、Selected as Top 20 Most Downloaded Article）
2009
[21] 篠崎陽一*、住友弘二、津田誠、小泉修一、井上和秀、鳥光慶一：
高速原子間力顕微鏡を用いた受容体の一分子イメージング
日薬理誌(Folia Pharmacol. Jpn.) 134巻, 2号, 68-72, 2009年 (表紙掲載)
[20] Shinozaki Y
Direct observation of dynamics in receptor protein by atomic force microscopy
[19] 篠崎陽一
主役登場 バイオの力を通信に
NTT技術ジャーナル, 21巻, 6号, 40, 2009年
[18] 篠崎陽一
原子間力顕微鏡による受容体タンパク質の動的構造解析
NTT技術ジャーナル, 21巻, 6号, 20-23, 2009年
[17] Shinozaki Y*, Sumitomo K, Tsuda M, Koizumi S, Inoue K, Torimitsu K.
Direct observation of ATP-induced conformaitonal changes in single P2X4 receptors
PLoS Biol. 2009, 7(5) e1000103. *corresponding author (Press Release)
[16] Sumitomo K*, Shinozaki Y, Takagi D, Nakashima H, Kobayashi Y, Torimitsu K.
AFM observation of membrane proteins suspended over CNT network.
Jpn. J. Appl. Phys., 2009, 48: 08JB18.
[15] 河西奈保子、C. S. Ramanujan, 篠崎陽一、住友弘二、J. F. Ryan, 鳥光慶一
受容体タンパク質の一分子観察
化学とマイクロ・ナノシステム研究会誌、2009年、第8巻、第1号、1-6
2008
[14] Siitonen AM, Sumitomo K*, Ramanujan CS, Shinozaki Y, Kasai N, Furukawa K, Ryan JF, Torimitsu K.
Elasticity of the purple membrane measured by atomic force microscopy
Appl. Surf. Sci. 2008, 254; 7877-7880.
[13] 鳥光慶一、篠崎陽一、河西奈保子、住友弘二
単一分子レベルでのたんぱく質の機能評価
[12] Shinozaki Y*, Siitonen AM, Sumitomo K, Furukawa K, Torimitsu K.
Effect of calcium ions on lipid dynamics analyzed with fast scanning atomic force microscopy
Jpn. J. Appl. Phys. 2008, 47(7): 6164-6167.
[11] Suzuki T, Tamehiro N, Sato Y, Kobayashi T, Ishii-Watanabe A, Shinozaki Y, Nishimaki-Mogami T, Hashimoto T, Asakawa Y, Inoue K, Ohno Y, Yamanagushi T, Kawanishi T.
The novel compounds that activate farnesoid X receptor: the diversity of their effects on gene expression
J. Pharmacol. Sci. 2008, 107(3): 285-294.
2007
[10] Koizumi S, Shigemoto-Mogami Y, Nasu-Tada K, Shinozaki Y, Ohsawa K, Tsuda M, B. Joshi BV, Jacobson KA, Kohsaka S, Inoue K*
UDP acting at P2Y6 receptors is a mediator of microglial phagocytosis
[9] Shinozaki Y, Sato Y*, Koizumi S, Ohno Y, Nagao T, Inoue K.
Retinoic acids acting through retinoid receptors protect hipppocampal neurons from oxygen-glucose deprivation-mediated cell death by inhibition of JNK and p38 mitogen-activated protein
Neuroscience, 2007, 147(1):153-63.
[8] Ishida S, Tanabe H, Shinozaki Y, Koyano S, Kagechika H, Shudo K, Ozawa S, Sawada JI, Ohno Y, Inoue K.
How DNA microarray technology contributes to the retinoid evaluations
In "Vitamin A: a new research", Nova Science Publishers, 2007
2006
[7] Shinozaki Y, Koizumi S, Ohno Y, Nagao T, Inoue K*
Extracellular ATP counteracts the ERK1/2-mediated death-promoting signaling cascades in astrocytes.
GLIA 2006, 54: 606-618 (GLIA 2005, 2006の一連の研究成果が日本薬理学会誌2007年9月号の表紙に掲載）
[6] Suzuki T, Nishimaki-Mogami T, Kawai H, Kobayashi T, Shinozaki Y, Sato Y, Hashimoto T, Asakawa Y, Inoue K, Ohno Y, Hayakawa T, Kawanishi T*
Screening of novel nuclear receptor agonists by a convenient reporter gene assay system using green fluorescent protein derivatives.
2005
[5] Shinozaki Y, Koizumi S, Ishida S, Sawada JI, Ohno Y, Inoue K*
Cytoprotection against oxidative stress-induced damage of astrocytes by extracellular ATP via P2Y1 receptors.
2004
[4] Ishida S, Shigemoto-Mogami Y, Shinozaki Y, Kagechika H, Shudo K, Ozawa S, Sawada JI, Ohno Y, Inoue K*
Differential modulation of PI3-kinase/Akt pathway during all-trans retinoic acid- and AM80-induced HL-60 cell differentiation revealed by DNA microarray analysis
Biochem. Pharmacol. 2004, 68: 2177-2186.
2002
[3] Shinozaki Y, Fukamiya N*, Uchiyama C, Okano M, Tagahara K, Bastow KF, Lee KH
Multidrug resistance cancer cells susceptibility to cytotoxic taxane diperpenes from Taxus yunnanensis and Taxus chinensis.
Bioorg. Med. Chem. Lett. 2002, 12: 2785-2788.
[2] Shinozaki Y, Fukamiya N*, Okano M, Nehira T, Tagahara K, Zhang SX, Lee KH
Dantaxusins C and D, two novel taxoids from Taxus yunnanensis.
J. Nat. Prod. 2002, 65: 371-374.
2001
[1] Shinozaki Y, Fukamiya N*, Fukushima M, Okano M, Nehira T, Tagahara K, Zhang SX, Zhang DC, Lee KH
Dantaxusins A and B, two new taxoids from Taxus yunnanensis.
J. Nat. Prod. 2001, 64: 1073-1076.
Review
[4] Shinozaki Y, Saito K, Kashiwagi K, Koizumi S
(title)
Neuropharmacol 2022, ##
[3] 篠崎陽一、パラジュリビジェイ、小泉修一
(title)
ファルマシア 2022, ##
[2] Shinozaki Y, Koizumi S.
Potential role of astrocytes and Muller cells in the pathogenesis of glaucoma (Review)
J Pharmacol Sci, 2021, 145: 262-267.
2020
[1] 篠崎陽一, 小泉修一.
グリア細胞の機能異常による緑内障発症メカニズム
Patent
発明届記載の発明の名称︓シナプスコネクターCPTXの緑内障への応⽤
出願時の発明の名称︓⼈⼯興奮性シナプスコネクタの緑内障への応⽤
出願⼈︓慶應義塾大学・東京都医学総合研究所
出願対象国︓PCT出願（⽶国仮出願63/701,055を基礎とする）
出願番号︓PCT/JP2025/034547
Title: THERRAPEUTIC AGENT FOR OPTIC NERVE DISEASES AND TREATMENT THEREOF
Inventors: Michisuke Yuzaki, Keiko Matsuda, Takayuki Harada, Youichi Shinozaki, Kazuhiko Namekata
Application number: 63/701,055
Application date: September 30, 2024（米国仮出願）
Title: MICROGLIA-CONTAINING INTRA-CNS TRANSFER AGENT, CNS DISEASE THERAPEUTIC AGENT INCLUDING SAME, MICROGLIA-INTRODUCED ANIMAL, AND METHOD FOR PRODUCING SAME
Inventors: Schuichi Koizumi, Parajuli Bijay, Eiji Shigetomi, Youichi Shinozaki
Application number: PCT/JP2021/040416
Application date: November 2, 2021（国外）
発明の呼称：ミクログリア含有中枢神経系内移動剤、及びこれを含む中枢神経系疾患治療薬、並びに ミクログリア導入動物、及びその製造方法
発明者：小泉修一、パラジュリ ビージェイ、繁冨英治、篠崎陽一
出願番号：特願2020-196642
出願者：国立大学法人山梨大学
発明の呼称：正常緑内障モデル、及び評価対象薬剤の正常眼圧緑内障予防乃至治療効果の評価方法
発明者：篠崎陽一、柏木賢治、小泉修一
出願番号：特開2019-216636
出願者：国立大学法人山梨大学
発明の呼称：緑内障モデル、評価対象薬剤の緑内障予防乃至治療効果の評価方法、及び眼圧調整剤
発明者：篠崎陽一、柏木賢治、小泉修一
出願番号：特開2017-051181
出願者：国立大学法人山梨大学
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