Real-Time PCR (StepOnePlus)
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Workflow of the StepOnePlus Real-Time PCR Instrument
At the Halal Science Centre, the StepOnePlus Real-Time PCR system is a core technology used to detect non-halal DNA (such as pork or non-syariah compliant animal derivatives) in food, cosmetics, and pharmaceutical products.
Phase 1: Sample DNA Extraction
Phase 1: Sample DNA Extraction
The genetic material (DNA) is isolated and extracted from the product sample (e.g., processed meat, gelatin, or seasoning).
Phase 2: Reaction Setup & Specific Amplification
Phase 2: Reaction Setup & Specific Amplification
The extracted DNA is mixed with specific reagents (primers and fluorescent probes) designed to target only non-halal animal DNA. The machine then performs precise heating and cooling cycles to duplicate (amplify) the target DNA millions of times.
Phase 3: Real-Time Fluorescence Detection
Phase 3: Real-Time Fluorescence Detection
As the target DNA multiplies, a built-in optical system detects the emission of a light signal (fluorescence) in real-time.
Positive Result (Contaminated): The fluorescence graph rises significantly, indicating the presence of non-halal DNA (e.g., pork).
Negative Result (Safe/Halal): The graph remains flat, indicating the sample is entirely free from the tested non-halal DNA.
QIAcube Connect Machine
QIAcube Connect Machine
QIAcube Connect is an automated machine designed to perform DNA/RNA extraction using QIAGEN spin-column technology. It automates steps that are normally done manually by laboratory technicians.
QIAcube Connect is an automated machine designed to perform DNA/RNA extraction using QIAGEN spin-column technology. It automates steps that are normally done manually by laboratory technicians.
Its Role in Halal Testing
In halal laboratories, the machine is commonly utilized for detecting non-halal biological materials, particularly through DNA analysis.
For instance, pork and pork-derived ingredient detection. The machine can extract DNA from food samples such as meat products, sausages, processed foods, Gelatin products, dairy products, and processed ingredients. After DNA extraction, the DNA is analyzed using techniques such as PCR (Polymerase Chain Reaction), Real-time PCR (qPCR), and DNA sequencing. This allows the laboratory to ascertain whether DNA from prohibited species (for example, pig) is present or not.
Additionally, the machine can be used for species identification.
The system enables the preparation of DNA samples for identifying beef, chicken, fish, pork, and other animal species.
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