To ensure faithful genome maintenance, chromatids have to be properly segregated to daughter cells. This requires the removal of all physical connections between sister chromatids before cells divide. Besides cohesins, which act as proteinaceous glue, a variety of other chromatid connections have to be equally removed. These include intermediates of DNA recombination such as Holliday Junctions, points at which recombined chromatids have become intertwined, and loci that have not replicated by the time cells reach the metaphase-anaphase transition. Cytologically, the connections between anaphase chromatids can take the form of chromatinized or ultrafine DNA bridges and such connections are frequently found in dividing cells, even when not treated with DNA damaging agents. Failure to process chromatin bridges impedes the segregation of chromatids and leads to genome instability, aneuploidy, and polyploidization.