Our research

Antibodies protect vertebrate animals and humans  from infections and toxins. Antibodies are proteins made by specialized cells, the B lymphocytes. During immune responses, the antibody genes are modified by programmed mutagenesis. This process takes place in the B cells that enter special anatomical structures named germinal centers. Complex cell-cell interactions and fate decisions inside germinal centers allow the selection of those B cells in which gained mutations leading to higher affinity of the antibody compared to other B cells.

More in detail, germinal center B cells modify the affinity of their antibody molecules by altering the sequence of the "variable" exon of the immunoglobulin genes by the mechanism of somatic hypermutation. Somatic hypermutation is initiated by Activation Induced Deaminase (AID), a unique enzyme that mutates DNA by transforming cytosine bases to uracils (a base normally found in RNA). 

AID also triggers the mechanism of class switch recombination, which remodels the locus of the antibody's heavy chain gene to swap exons that define the default IgM class for new ones, such as those defining IgG, IgE or IgA. Each antibody class has different biological properties, specialized to fight different threats.

Why does it matter? 

The mechanisms regulating the activity of AID, somatic hypermutation, class switch recombination, and the germinal center function, are all necessary for efficient immune responses to infection and vaccines, as well as for the generation of the immunological memory that confers long-term protection. Their dysfunction can result in immunodeficiency, autoimmunity, or B cell lymphoma.

Ongoing research projects : 

• • Discovering and characterizing mechanisms regulating AID activity.

  • Studying mechanisms regulating germinal center B cell function.

  • Characterizing enzymes regulating the incorporation of uracil into the DNA.