The Tissue Engineering and Cell Models Core (TECM) enables DDRCC investigators to address key questions relating to causality and mechanisms by providing novel and vetted experimental models that can be customized to their needs. The TECM has three major components: (1) A centralized repository and facility for established and primary gut-relevant cell lines, which was originally part of the host-microbe core, but moved to the TECM to centralize all cell and tissue experimental systems, (2) A tissue engineering component that includes customizing and developing intestinal organoid technologies for specific applications needed by DDRCC users, and (3) an experimental systems component that includes in vivo models (rodent microsurgery and C. elegans), ex vivo models, and live functional assays.
Click through to see protocols for organoid preps, TPN and FACs