The first possible method by which a mutation occurs and DNA is altered is in a process called substitution. In this process, one base of DNA is actually exchanged for another. To see how this is significant, a change in a single codon (a sequence of three nucleotides which together form a single unit of genetic coding in a DNA molecule) which provides the coding for a single amino acid can cause an alteration in the protein being produced. The disease known as sickle-cell anemia is actually caused by one of these codon changes. The substitution in the beta-hemoglobin gene eventually alters one amino acid in the protein produced. Sickle-cell anemia can be a very serious condition (where red blood cells form in a “sickle” shape) and can lead to chronically low oxygen levels in the blood.
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Mutation par Substitution from Wikimedia Commons
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Insertions are another method of a mutation occurrence in which extra base pairs are inserted into a new section of a DNA strand. So, for example, in a strand of DNA consisting of the sequence “ACCTGGA”, an insertion may cause the strand to then appear as “ACCGTATGGA.” The insertion of the “GTA” is a mutation in this scenario. A deletion, on the other hand, occurs when a particular section of a strand of DNA is deleted and removed. For instance, a deletion in the sequence “ACCTGGA” might then become “ACCTA” after the deletion of “GG”. The final form of a mutation is referred to as a frameshift mutation. Frameshift mutations are mutations in which an insertion or deletion causes a gene to no longer be comprehensible. At the level of the DNA strand, the incorrect sequence of codons could lead the gene to become incomprehensible. This goes to show just how significant one minor change in genetic sequencing can be.
References
Lodish, H., Berk, A., Zipursky, S. L., Matsudaira, P., Baltimore, D., & Darnell, J. (2000). Viruses: Structure, function, and uses. Molecular Cell Biology. 4th Edition, Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK21523//