Course responsible: Andre S. Ribeiro
Learning Outcomes: In this course, the student will learn about advanced mechanisms of regulation of prokaryotic, mitochondrial, and eukaryotic gene expression and how they affect phenotypes, while interacting with the environment. The lectures will be given by specialists in each field covered, from the simpler to the more complex, and will focus on recent findings. In the end, the students should be able to conceive and implement methods from functional genomics to address research challenges in this and similar areas of research. They should also understand biological adaptations to global environmental changes. Moreover, the students should be able to use the knowledge to execute projects and pitches in biotechnology and biomedicine topics.
Prerequisites: To attend this course, the students should have attended basic university courses on Cell Biology, Molecular Genetics, and Molecular Biology.
Remarks: The course is suitable for postgraduate studies.
Course responsible: Andre S. Ribeiro
Learning Outcomes: The student will be introduced to experimental techniques and analysis tools in single cell biology studies. The aim is to provide understanding of techniques of single cell observation and how to interpret the results. The focus will be the manipulation and measurements of gene expression in prokaryotic systems using live cell multi-modal microscopy systems and flow cytometry. The model organism will be Escherichia coli. Finally, students will gain understanding of the objectives, applicability and limitations of the methods used in quantitative single cell biology studies. Several lectures will be in a laboratory, so that the students can learn how to handle devices, such as microscopes and flow cytometers.
Prerequisites: Basic knowledge of molecular biology and/or systems biology. .
Remarks: The course is suitable for postgraduate studies.