Gene Libraries

1. Library of fluorescent transcriptional reporters of native global regulators of E. coli’s gene network. It reports on 16 genes, each expressing a protein that regulates 45 or more genes, as described in RegulonDB (as of 27.05.2019). This library is a collection of E. coli strains, each harboring a single-copy plasmid (pBAC) coding for a green fluorescent protein (GFPmut3b) under the control of the full-length copy of the native promoter. The library allows measuring temporal promoter activities.

Publication DOI: 10.1128/msystems.00065-24

Repository: AddGene: https://www.addgene.org/Andre_Sanches_Ribeiro/

2. Library of a small number of synthetically engineered closely spaced promoters in tandem formation, fluorescently tagged.

Publication DOI: 10.1021/acs.biochem.4c00012

Repository: AddGene: https://www.addgene.org/Andre_Sanches_Ribeiro/

Plasmid-borne constructs

1. PtetA-tsr-venus: Tsr-venus proteins expressed under the control of a TetA promoter, inducible by aTc (DOI: 10.1016/j.bpj.2016.10.024)

2. Plac-tsr-venus: Tsr-venus proteins expressed under the control of a Lac promoter, inducible by IPTG (DOI: 10.1016/j.bpj.2016.10.024)

3. Plac-ara-1-mCherry-MS2-BSs: mCherry proteins followed by binding sites for MS2-GFP proteins under the control of a Lac-Ara-1 promoter inducible by IPTG and/or Arabinose (DOI: 10.1093/dnares/dsw009)

4. PL-lacO1-mCherry-MS2-BSs: mCherry proteins followed by binding sites for MS2-GFP proteins under the control of a LacO1 promoter inducible by IPTG (DOI: 10.1016/j.bbagrm.2018.12.005)

5. pZA24-PL-lacO1-FtsZ-mTagBFP2: Low-copy plasmid producing fused proteins FtsZ-BFP2 under the control of PL-lacO1 promoter (DOI: 10.1088/1478-3975/aac1cb)

6. PL-tetO1-mCherry-MS2-BSs: Single-copy plasmid producing RNA coding for mCherry and for binding sites for MS2-GFP proteins under the control of PLTetO promoter inducible by aTc. (DOI: 10.1016/j.bbagrm.2016.07.011 )

7. PTet-PLac-mCherry-MS2-BSs: Single-copy plasmid producing RNA coding for mCherry and for binding sites for MS2-GFP proteins under the control of Tet-Lac promoter inducible by IPTG and aTc. (DOI: 10.1016/j.bbagrm.2018.12.005)

8. PRham-rpoD: rpoD expressed under the control of PRham inducible by Rhamnose (DOI: 10.1016/j.bbagrm.2020.194515)

9. PRham-gyr-sfGFP: Gyrase tagged with sfGFP expressed under the control of PRham inducible by Rhamnose (DOI: doi: 10.1016/j.bbagrm.2020.194515).

Chromosomal-integrated constructs

1. Lac-mCherry-MS2-BSs: Lac promoter controlling the expression of mCherry-MS2-BSs cassette inserted into the lac locus of E. coli strain BW25993 (DOI: 10.1038/s41598-019-39618-z)

2. Lac mutants-mCherry-MS2-BSs (4 mutants): Various Lac promoter variants controlling the expression of binding sites for MS2-GFP in the E. coli strain BW25993 (DOI: 10.1016/j.bbagrm.2018.12.005)