Research

Structure-function analysis of human SSB protein domains 

How do these proteins access ssDNA?

What sites of DNA do these proteins localize to in cells?

What are the determinants of these functions?

Techniques: In vitro characterization of protein function, CRISPR screening approaches

Identify protein-protein interactions or complexes of human SSBs 

What protein-protein interactions are important for SSB functions?

What is the function of these interactions during DNA replication?

What are the phenotypic consequences of these interactions?

Techniques: isolation of proteins on nascent DNA (iPOND), TurboID, APEX2

Expand to other human SSBs and potential for translation

Are there other DNA binding proteins that are important in these processes?

Can these proteins inform on activity of therapeutic agents?

Techniques: cell viability assays, patient derived tumor models, small molecule inhibitor design