LAB TECHNIQUES

ANTIMICROBIAL ACTIVITY

• Agar Plug Diffusion Method

• Agar Well Diffusion Method

• Cross-Streak Method

• Perpendicular Streak Method

• Minimum Inhibitory Concentration Testing

• Time-Kill Assay

ANTIMICROBIAL SUSCEPTIBILITY

• Disk Diffusion Method 

• Broth Dilution Method

• Agar Dilution Method

BIOCHEMICAL TESTS

• Carbohydrate Fermentation Tests

• Catalase Test

• Citrate Utilization Test

• Indole Test

• Oxidase Test

• Methyl Red and Voges-Proskauer Tests

• Triple Sugar Iron  Test

BIOINFORMATICS

• Primer Design

• Phylogenetic Analysis

• Multilocus Sequence Typing

• Microarray Analysis

• Comparative Genomic Analysis

• Operational Taxonomic Unit Clustering

• Protein Structure Prediction 

• Molecular Docking 

• Structure Visualization

• Homology Modeling

CORE TECHNIQUES

• Aseptic Technique

• Isolation and Purification of Bacteria

  • Serial Dilution

  • Spread Plate Method

  • Streak Plate Method

  • Pour Plate Method

• Microbial Culture

  • Aerobic Culture 

  • Microaerophilic Culture

• Colony Morphology Characterization

• Quantification of Microbes 

  • Viable Cell Count

  • Most Probable Number  Method

  • Spectrophotometry 

  • Direct Microscopic Count

• Growth Curve Analysis

MICROSCOPY 

• Brightfield Microscopy

• Phase Contrast Microscopy

• Electron Microscopy (outsourced)

MOLECULAR MICROBIOLOGY

• DNA Extraction

• Gel Electrophoresis

• Polymerase Chain Reaction

• DNA Sequencing (outsourced)

PATHOGENICITY TESTS

• Coagulase Test

• DNase Test

• Motility Test

• Adhesion Assays

• Biofilm Formation Assay

• Oxidative Stress Assays

STAINING TECHNIQUES

• Simple Staining

• Negative Staining

• Gram Staining

• Capsule Staining

• Endospore Staining

• Flagella Staining

VIROLOGY

• Bacteriophage Isolation 

• Plaque Assay

OTHER TECHNIQUES

• Microbiological Analysis of Water

• Protozoa Isolation from Environmental Samples

• Sporulation and Germination Assays

• Winogradsky Column Preparation

• Yeast and Fungal Culture