Design Overview

Our project divides the goals into two main subprojects: axial stretch and radial stretch (or pressurization). In each subproject, we wanted to implement the desired stretch and validate the implemented stretch by measuring it. 

Axial stretch was implemented using a rack and pinion gear system with an ratchet and pawl inspired locking mechanism. Radial pressure was implemented by static fluid injection, and was estimated assuming a diameter to pressure relationship using previously obtained pressure-diameter measurements. Measurements were taken by using standardized photographs that were input into a designed MATLAB imaging processing script to ensure accuracy for the small vessels. To ensure microscope compatibility, the device was made from materials known to work with the multiphoton microscope and fits the size specifications specific to the microscope used in lab.

Our device provides a solid starting point for quantifying collagen fibers with in situ orientation, but we believe that increased resolution, increased consistency, and further validation of assumptions should be completed before use in research.