LAB REPORT 3

COLLECTION OF PLANT DISEASE SAMPLES

INTRODUCTION

Plant diseases are caused by various pathogens such as fungi, bacteria, viruses, nematodes, and other microorganisms, as well as environmental factors that can damage plants. The disease can affect any part of the plant, including roots, stems, leaves, and flowers, leading to reduced growth, lower yields, and in severe cases, plant death.Disease diagnosis is the investigative act of biotic or abiotic disease identification

Early detection and effective management practices are important to reduce the spread and impact of plant diseases on other plants. Correct collection techniques are important to ensure that the data obtained can be used for further treatment or research. Plant disease samples should be stored according to standard procedures to avoid contamination, preserve the integrity of the sample and prevent the spread of the pathogen to other plants.

OBJECTIVE

To collect the diseased plants with visible symptoms and signs in the field.
To preserve the diseased plant samples for isolation and identification in the laboratory.

MATERIALS

Plastic bag

Plant disease sample

Marker pen

Scissors

PROCEDURES

All plant parts such as leaves, branches, stems, fruits, and roots are examined for visible signs of disease. All infected plant parts were collected.
All tools and equipment that will be used to cut the specimen have been cleaned to avoid contamination and damage to the collected sample.
More than one plant sample was collected because one sample is not enough to obtain a correct and accurate diagnosis.
Dead plant parts should not be collected because it is difficult to separate the pathogen from the dead host.
Background information was recorded according to the field or sampling area in the given table.

RESULT

DISCUSSION

List four example of biotic and abiotic agents that caused plant diseases.

a) Biotic agent

-Nematodes

-Fungi

-Bacteria

-Mycloplasmas

b) Abiotic agent

-Weather

-Soil

-Chemicals

-Cultural practices

2. Summarize all the important processes in plant disease collection using a simple diagram or flowchart.

CONCLUSION

In conclusion, we were able to collect diseased plants with visible symptoms and signs in the field. We can also find out whether it is caused by abiotic or biotic agents. By collecting symptomatic plants, we can accurately diagnose the causative pathogen to understand the nature of the disease. The samples obtained must also be well maintained and meticulous to obtain accurate results. This method's significance in clinical and scientific settings is demonstrated by its successful application in experiments.

REFERENCES

Brathwaite, C. W. D. (1981). An introduction to the diagnosis of plant disease. Inter-American Institute for Cooperation on Agriculture.
Gardener, M. (n.d.). Abiotic Plant Diseases & Causes. FastGrowingTrees.com. https://www.fast-growing-trees.com/pages/abiotic-plant-disease
Plant Disease Diagnosis. (2019). Plant Disease Diagnosis; Plant Disease Diagnosis. https://www.apsnet.org/edcenter/disimpactmngmnt/casestudies/Pages/PlantDiseaseDiagnosis.aspx
Plant Disease: Pathogens and Cycles. (2016, December 19). CropWatch. https://cropwatch.unl.edu/soybean-management/plant-disease

Page updated

Report abuse