Sterilisation was carried out to remove any potential pollutants from the surface of the work table. Clean the area and gloves using 70% of alcohol. Turned on the Bunsen burner.

For the bacterial culture, do a serial dilution 10 times. 100 µL of the stock microbial solution is serially transferred into vials or test tubes containing 900 µL of water, with careful mixing following each dilution step, to achieve a ten-fold dilution on a 1 mL volume.

Used an inoculating loop to practise the bacterial streaking and spreading technique on nutrient agar.  Used a Bunsen burner to sterilise an inoculating loop or needle.

A loopful of sample is streaked on the first quadrant in four streaking on the agar plate. Close the lid of the plate after streaking.

For the spreading technique, used a L - shaped glass rod. The spreader is sterilized by dipping it into a beaker of ethanol then passing it through the flame of the Bunsen burner to ignite excess ethanol. 

Label the name, date , ST (streaking) and SP (spread) on the lid and sealed with sellotape to avoid contamination. 

Incubate the plates of agar in the incubator at set temperature of 25°C .

Observe the plates and count the single bacterial colony after 48 hours of incubation.