LAB REPORT 2
PREPARATION AND OBSERVING SPECIMEN MOUNTED ON SLIDE UNDER LIGHT MICROSCOPE
PREPARATION AND OBSERVING SPECIMEN MOUNTED ON SLIDE UNDER LIGHT MICROSCOPE
Bacteria are single-celled microorganisms that do not have a nuclear membrane. It is also metabolically active and is divided by binary fission. Nevertheless, bacteria appear to be a relatively simple form of life because they easily adapt to environmental conditions. Bacteria are found almost everywhere on Earth and are essential to the planet's ecosystems. Fungal, singular, fungi, are a group of eukaryotic, non-phototrophic organisms with rigid cell walls, including mushrooms, molds and yeasts. Yeast is a microscopic fungus consisting of solitary cells that reproduce by budding. It is an organism found in Kingdom fungi contains cell walls and is ubiquitous. They are classified as heterotrophs among living organisms. Both bacteria and fungi are microorganisms that cannot be seen by the human eye. This is because most microorganisms are much smaller than 0.1mm and a microscope must be used to observe them directly. In general, the diameter of microorganisms ranges from 0.2 - 2.0 microns.
To practice mounting the specimen on a glass slide.
To examine the specimens under light microscope.
Fungal culture
Bacteria culture
Sterille distilled water
Inoculating loop & needle
Cover slip
Glass slide
Lactophenol cotton blue (LCB)
Compound light microscope
The sterilization procedure were performed to eliminate all possible contaminants on the working bench surface and in the laminar air flow. The sterilized utensils was used.
The fungal spores was mount on glass slides with a drop of LCB and covered with a cover slip.
The bacterial cells was mount on glass slides with a drop of water and covered with a cover slip.
The slide was placed under a compound light microscope
The spore and cell structures were observed and the results had been recorded.
These fungi have cylindrical shapes or fungal thread-like structures ranging from as small as 0.5 to 20 µm, with most being between 2 and 10 µm in diameter.
Bacteria appear as small dots, rods or circles under the microscope. For rod-shaped or filamentous bacteria, the length is 1-10 µm and the diameter is 0.25-1.
Bacteria are also known as producers and decomposers. Examples of producers are chemosynthetic bacteria and photosynthetic bacteria while decomposers are soil bacteria. In addition, the characteristic of bacteria is that they do not have a nucleus and cell organelles, instead the cell wall is made of peptidoglycan. it can also live in an area with a neutral pH value such as 6.5 to 7.0.
On the other hand, fungi are known as decomposers. Additionally, fungi are characterized by having a nucleus and cell organelles, while the cell wall is made of chitin. It can also live in areas with slightly acidic pH values such as 4.0 to 6.0. The transmission occurs through spores and the source of energy for them is to feed on both living and dead cells.
In conclusion, we were able to practice using specimens on glass slides while correctly placing bacteria and fungi. Next, the results of the specimen under the light microscope can also be clearly seen in the results section. Preparation and observation under the light microscope requires careful preparation of the specimen, to ensure that the specimen can be seen clearly. It is very important for us to do this experiment carefully because each microorganism has different properties and characteristics.
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Aryal, S. (2018, June 11). Different Size, Shape and Arrangement of Bacterial Cells. Microbiology Info.com. https://microbiologyinfo.com/different-size-shape-and-arrangement-of-bacterial-cells/
McGinnis, M. R., & Tyring, S. K. (1996). Introduction to Mycology. Nih.gov; University of Texas Medical Branch at Galveston. https://www.ncbi.nlm.nih.gov/books/NBK8125/
Rogers, K., & Kadner, R. J. (2018). Bacteria. In Encyclopædia Britannica. https://www.britannica.com/science/bacteria