Unit 4 - Evolution

How long can you survive? Who Wants to Live a Million Years, the Charles Darwin game of survival.

BLAST Lab Information and Links

Part B

1. Copy the nucleotide sequence below for the mouse GULO gene.

> mouse gulo gene CDS

ATGGTCCATGGGTACAAAGGGGTCCAGTTCCAAAACTGGGCGAAGACCTATGGCTGCAGTCCAG

AGATGTACTACCAGCCCACATCAGTGGGGGAGGTCAGAGAGGTGCTGGCCCTGGCCCGGCAGC

AGAACAAGAAAGTGAAGGTGGTGGGTGGCGGCCACTCGCCTTCAGACATCGCCTGCACCGATG

GCTTCATGATTCACATGGGCAAGATGAACCGGGTTCTCCAGGTGGACAAGGAGAAGAAGCAGG

TCACAGTGGAAGCCGGTATCCTCCTGACTGACCTGCACCCACAGCTGGACAAGCATGGCCTGGC

CCTGTCTAATCTGGGAGCCGTGTCTGATGTGACGGTTGGTGGCGTCATTGGGTCTGGAACACATA

ACACCGGGATCAAGCACGGTATCCTGGCCACCCAGGTGGTGGCCCTGACCCTGATGAAGGCTGT

GGAACAGTTCTGGAATGTTCTGAGTCAAGTAATGCAGATGTGTTCCAGGCTGCAAGGGTGCACC

TGGGCTGCCTGGGTGTTATCCTCACTGTCACCCTGCAGTGTGTGCCACAGTTCCACCTTCTGGAG

ACATCCTTTCCTTCGACCCTCAAGGAGGTCCTTGACAACCTGGACAGCCACCTGAAGAAGTCTG

AGTACTTCCGCTTCCTCTGGTTTCCTCACAGTGAGAACGTCAGCATCATCTACCAAGATCACACC

AACAAGGAGCCCTCCTCTGCATCTAACTGGTTTTGGGACTATGCCATTGGGTTCTACCTCCTGGA

ATTCTTGCTCTGGACCAGCACCTACCTGCCACGCCTCGTGGGCTGGATCAACCGCTTCTTCTTCT

GGCTGCTGTTCAACTGCAAGAAGGAGAGCAGCAACCTCAGCCACAAGATCTTCTCCTACGAGTG

TCGCTTCAAGCAGCATGTCCAAGACTGGGCCATCCCCAGGGAGAAGACCAAGGAGGCCCTGCTG

GAGCTAAAGGCCATGCTGGAGGCCCACCCCAAGGTGGTAGCCCACTACCCCGTGGAGGTGCGCT

TCACCCGAGGTGATGACATCCTGCTGAGCCCGTGCTTCCAGAGGGACAGCTGCTACATGAACAT

CATTATGTACAGGCCCTATGGGAAGGATGTGCCTCGGTTGGATTACTGGCTGGCCTATGAGACC

ATCATGAAGAAGTTTGGAGGCAGGCCCCACTGGGCAAAGGCCCACAATTGCACCAGGAAGGAC

TTTGAGAAAATGTACCCCGCCTTTCACAAGTTCTGTGACATCCGCGAGAAGCTGGACCCCACTG

GAATGTTCTTGAATTCGTACCTGGAAAAGGTTTTCTACTAA

2. On the internet, go to www.ncbi.nlm.nih.gov.

1. Copy the mouse GULO protein below > mouse GULO protein

MVHGYKGVQFQNWAKTYGCSPEMYYQPTSVGEVREVLALARQQNKKVKVVGGGHSPSDIACTDG

FMIHMGKMNRVLQVDKEKKQVTVEAGILLTDLHPQLDKHGLALSNLGAVSDVTVGGVIGSGTHNT

GIKHGILATQVVALTLMKADGTVLECSESSNADVFQAARVHLGCLGVILTVTLQCVPQFHLLETSFPS

TLKEVLDNLDSHLKKSEYFRFLWFPHSENVSIIYQDHTNKEPSSASNWFWDYAIGFYLLEFLLWTSTY

LPRLVGWINRFFFWLLFNCKKESSNLSHKIFSYECRFKQHVQDWAIPREKTKEALLELKAMLEAHPK

VVAHYPVEVRFTRGDDILLSPCFQRDSCYMNIIMYRPYGKDVPRLDYWLAYETIMKKFGGRPHWAK

AHNCTRKDFEKMYPAFHKFCDIREKLDPTGMFLNSYLEKVFY

Part 3: Search the databases and create your own phylogeny

UniProt Pre-Lab Tutorial

https://www.youtube.com/watch?v=iJGdhSqrTKI&list=HL1395263867

UniProt.

www.uniprot.org

Phylogeny Analysis

www.phylogeny.fr

T-­‐coffee website:

http://tcoffee.vital-it.ch/apps/tcoffee/do:regular