Description of the LEMeB equipment park and environments December 2023

The Laboratory of Metabolic and Bioprocess Engineering (LEMeB) is one of the laboratories of the Department of Food Engineering (DEA) of the Faculty of Food Engineering (FEA) at UNICAMP. The physical structure of LEMeB is made up of three laboratories, five auxiliary rooms, and an access corridor. 

Laboratories

Laboratory 1 is a room measuring 8 x4.70 m. This laboratory has three benches and two sinks. The equipment includes electrophoresis tanks, photodocumenter, thermocycler, benchtop centrifuge, NanoDrop, spectrophotometer, thermoblock, thermoblock with agitation, ELISA plate reader and TECAN plate reader. Under the countertops there are cabinets and drawers with Formica coverings where materials and glassware are stored. Also in this laboratory are shaker incubators and B.O.D. Incubator. This environment has split air conditioning equipment that maintains the laboratory temperature at 22°C. 

Laboratory 2, measuring 8x3 m, is equipped with four aerobic fermenters in addition to two duplex refrigerators, a refrigerator, two domestic freezers and an ultrafreezer for storing samples and reagents. This environment has split air conditioning equipment that maintains the laboratory temperature at 22°C. 

Laboratory 3, measuring 7.30x16.41m, has two Split-type air conditioning equipment to maintain the ambient temperature. This laboratory includes a fume hood, biological safety cabinet, laminar flow cabinet, safety shower, a CO2 fire extinguisher, refrigerated centrifuge, shaker incubator, ultrafreezer and a duplex refrigerator. There are Formica-lined cabinets for storing reagents. On the benches are a spectrophotometer, water purification and ultrafiltration equipment, a bench centrifuge, a micro centrifuge, a water bath, a pH meter, a dispenser, a diluter, and a microwave. 

Rooms

Room 1 is an administration room and office. 

Room 2 (HPLC Room) with dimensions of 2.28x3.58m is maintained at 22ºC and has two high-efficiency liquid chromatographs for detecting sugars, organic acids and alcohols, one being an Accela® HPLC with refractive index detector. and a DIONEX® with electrochemical gold detector with pulsed amperometry. 

Room 3 was created to separate noisy equipment such as freeze dryer, ice machine, microbiological oven, sterilization oven with forced circulation, ultrasound bath, vacuum pump, ultra-thermostatic water bath, ultrafiltration cell. Under the countertops there are cabinets and drawers with Formica coverings where disposable materials are stored, as well as some small equipment such as magnetic stirrers, vortex machines and glassware. 

Room 4 (Autoclave Room) houses the 2 vertical autoclaves, a muffle furnace, a knife mill and a sieve shaker. 

Room 5 with dimensions of 3x3m has 2 analytical and 2 semi-analytical balances and AKTA (protein purification), equipment, reagents and filtration material are stored under the benches.

List of equipments in the equipment park at LEMeB - Dec 2023

#1 - Accela® Hplc With Refractive Index Detector

#1 - Akta (Protein Purification)

#2 - Analytical Balances

#1 - B.O.D Incubator

#2 - Bench Centrifuges

#1 - Biological Safety Cabinet

#1 - Diluter

#2 - Dionex® With Electrochemical Gold Detector With Pulsed Amperometry.

#2 - Direct-Q For Water Purification And Ultrafiltration

#2 - Domestic Freezers

#3 - Duplex Refrigerators

#2 - Electrophoresis Chamber

#1 - Elisa Plate Reader

#1 - Emergency Shower

#1 - Exhaust Hood

#1 - Freeze Dryer

#1 - Ice Machine

#1 - Knife Mill

#1 - Laminar Flow Cabin

#4 - Magnetic Stirrers

#1 - Micro Centrifuge

#3 - Microbiological cabinets

#1 - Microwave

#1 - Muffle

#1 - Nanodrop

#2 - pH Meter

#1 - Photodocumenter

#4 - Reactors (Fermenters)

#1 - Refrigerated Centrifuge

#1 - Refrigerator

#2 - Semi-Analytical Balances

#4 - Shaker Incubators

#1 - Sieve Shaker

#1 - Sterilization Oven With Forced Circulation

#1 - Tecan Plate Reader

#1 - Thermoblock

#2 - Thermoblocks With Stirring

#2 - Thermocyclers

#1 - Ultrafiltration

#2 - Ultrafreezers For Sample Storage

#1 - Ultrasound Bath

#4 - Ultra-Thermostatic Water Bath

#2 - Vacuum Pumps

#2 - Vertical Autoclaves

#2 - Visible Spectrophotometer

#2 - Vortex

#4 - Water Bath With Agitation