PUBLICATIONS
ARTICLES
2024
N. Sakamoto, K. Ito, S. Ii, D.E. Conway, Y. Ueda, J. Nagatomi: A homeostatic role of nucleus-actin filament coupling in the regulation of cellular traction forces in fibroblasts. Biomechanics and Modeling in Mechanobiology, 2024. (https://doi.org/10.1007/s10237-024-01839-1)
坂元 尚哉, 上野 竜汰, 大八木 貴史, 武居 直行:接着基質伸展に伴う細胞核動的変形挙動解析に向けたその場観察装置の開発.日本機械学会論文集,90巻,930号,p23-00299,2024.(https://doi.org/10.1299/transjsme.23-00299)
2023
K. Sawasaki, M. Nakamura, S. Imada, Y. Horie, K. Takahashi, R. Yamazaki, N. Kimura, K. Kawahito, N. Sakamoto: Disruptive Effect of Impinging Jet Flow Environment on the Integrity of Endothelial Monolayer. Journal of Biorheology, Vol. 37 (2), pp. 130-137, 2023. (https://doi.org/10.17106/jbr.37.130)
Y. Ueda, N. Sakamoto: A Combined Application of Cyclic Stretching and Spheroid Culture Promotes Endothelial Differentiation of Mesenchymal Stem Cells. Journal of Biorheology, Vol. 37 (2), pp. 88-95, 2023 (https://doi.org/10.17106/jbr.37.88).
T. Miyano, A. Suzuki, N. Sakamoto: Actin cytoskeletal reorganization is involved in hyperosmotic stress-induced autophagy in tubular epithelial cells. Biochemical and Biophysical Research Communications, Vol. 663, pp. 1-7, 2023. (https://doi.org/10.1016/j.bbrc.2023.04.070)
2022
M. Ohta, N. Sakamoto, K. Funamoto, Z. Wang, Y. Kojima, H. Anzai: Review of Functional Analysis of Endothelial Cells in Flow Chambers. Journal of Functional Biomaterials, Vol. 13, 92, 2022. (Review)(https://doi.org/10.3390/jfb13030092)
坂元 尚哉, 舘林 耕平, 三好 洋美:壁せん断応力―引張ひずみ組合せ刺激が間葉系幹細胞の内皮分化に及ぼす影響.日本機械学会論文集,88巻,991号,p22-00103,2022.(https://doi.org/10.1299/transjsme.22-00103)
T. Miyano, A. Suzuki, N. Sakamoto: Calcium influx through TRPV4 channels involve in hyperosmotic stress-induced epithelial-mesenchymal transition in tubular epithelial cells . Biochemical and Biophysical Research Communications, Vol. 617, pp. 48-54, 2022. (https://doi.org/10.1016/j.bbrc.2022.06.003)
Y. Hiroshima, Y. Oyama, K. Sawasaki, M. Nakamura, N. Kimura, K. Kawahito, H. Fujie, N. Sakamoto: A Compressed Collagen Construct for Studying Endothelial–Smooth Muscle Cell Interaction under High Shear Stress. Annals of Biomedical Engineering, Vol. 50, No. 8, pp. 951-963, 2022. (https://doi.org/10.1007/s10439-022-02972-7)
YE X, Sakamoto N, Kotake Y, Yamasaki M, Numao M, Fujie H: Microscopic observation of centrifugally compressed cell-collagen combined constructs (C6) developed for soft tissue repair. 臨床バイオメカニクス.
S. Tsukamoto, K.-H, Chiam, T. Asakawa, K. Sawasaki, N. Takesue, N. Sakamoto: Compressive forces driven by lateral actin fibers are a key to the nuclear deformation under uniaxial cell-substrate stretching. Biochemical and Biophysical Research Communications, Vol. 597, pp. 37-43, 2022 (https://doi.org/10.1016/j.bbrc.2022.01.107).
坂元尚哉:せん断応力が血管内皮細胞へ及ぼす影響.トライボロジスト,第1号,pp36~41,2022.(解説)
2021
T. Miyano, A. Suzuki, N. Sakamoto: Hyperosmotic stress induces epithelial-mesenchymal transition through rearrangements of focal adhesions in tubular epithelial cells. PLOS ONE, Vol 16, No. 12, e0261345. (https://doi.org/10.1371/journal.pone.0261345)
K. Matsushita, C. Nakahara, S. Kimura, N. Sakamoto, S. Ii, H. Miyoshi: Intranuclear mesoscale viscoelastic changes during osteoblastic differentiation of human mesenchymal stem cells. FASEB Journal, Vol 35, No. 12, e22071
S. Tsukamoto, T. Asakawa, S. Kimura, N. Takesue, M.R.K. Mofrad, N. Sakamoto: Intranuclear strain in living cells subjected to substrate stretching: A combined experimental and computational study. Journal of Biomechanics, Vol. 119, 110292, 2021
J. Pauty, S. Nakano, R. Usuba, T. Nakajima, Y. Johmura, S. Omori, N. Sakamoto, A. Kikuchi, M. Nakanishi, Y. Matsunaga: A 3D tissue model-on-a-chip for studying the effects of human senescent fibroblasts on blood vessels, Biomaterials Science, Vol. 9, pp. 199-211, 2021.
2019
X. Han, N. Sakamoto, N. Tomita, H. Meng, M. Sato, M. Ohta: Influence of TGF-β1 expression in endothelial cells on smooth muscle cell phenotypes and MMP production under shear stress in a co-culture model, Cytotechnology, Vo. 71, pp. 489-496, 2019.
2018
N. Sakamoto, Y. Ueki, M. Oi, T. Kiuchi, M. Sato: Fluid shear stress suppresses ICAM-1-mediated transendothelial migration of leukocytes in coculture model, Biochemical and Biophysical Research Communications, Vol. 502, pp. 403-408, 2018.
2017
D. Yoshino, N. Sakamoto, M. Sato: Fluid shear stress combined with shear stress spatial gradients regulate vascular endothelial morphology, Integrative Biology, Vol. 9 (7), pp.584-594, 2017.
N. Sakamoto, M. Ogawa, K. Sadamoto, M. Takeuchi, N. Kataoka: Mechanical role of nesprin-1-mediated nucleus-actin filament binding in cyclic stretch-induced fibroblast elongation. Cellular and Molecular Bioengineering, Vol. 10 (4), pp. 327-338, 2017 .
X. Han, N. Sakamoto, N. Tomita, H. Meng, M. Sato, M. Ohta: Influence of shear stress on phenotype and MMP production of smooth muscle cells in a co-culture model. Journal of Biorheology, Vol. 31 (2), pp. 50-56, 2017.
N. Sakamoto, K. Sadamoto: Effect of low oxygen conditions on matrix metalloproteinase-9 production of macrophages subjected to cyclic stretching: involvement of ERK and Rho kinase pathways. Journal of Biomechanical Science and Engineering, Vol. 12 (1), p. 16-00590, 2017.
2016
T. Ohashi, Y. Sugaya, N. Sakamoto, M. Sato: Relative contribution of physiological hydrostatic pressure and fluid shear stress to endothelial monolayer integrity. Biomedical Engineering Letters, Vol. 6 (1), pp. 31-38, 2016.
2015
H. Abiko, S. Fujiwara, K. Ohashi, R. Hiatari, T. Mashiko, N. Sakamoto, M. Sato, K. Mizuno: Rho guanine nucleotide exchange factors involved in cyclic-stretch-induced reorientation of vascular endothelial cells. Journal of Cell Science, Vol. 128 (9), pp. 1683-1695, 2015.
〜2013
K. Oya, N. Sakamoto, M. Sato: Hypoxia suppresses stretch-induced elongation and orientation of macrophages. Bio-Medical Materials and Engineering, Vol. 23 (6), pp. 463-471, 2013.
L. Yuan, N. Sakamoto, G. Song, M. Sato: Low-Level Shear Stress Induces Human Mesenchymal Stem Cell Migration Through the SDF-1/CXCR4 Axis via MAPK Signaling Pathways, Stem Cells and Development, Vol. 22 (17), pp. 2384-2393, 2013.
D. Yoshino, N. Sakamoto, K. Takahashi, E. Inoue, M. Sato: Development of Novel Flow Chamber to Study Endothelial Cell Morphology: Effects of Shear Flow with Uniform Spatial Gradient on Distribution of Focal Adhesion, Journal of Biomechanical Science and Engineering, Vol 8 (3), pp. 233-243, 2013.
L. Yuan, N. Sakamoto, G. Song, M. Sato: High-level shear stress stimulates endothelial differentiation and VEGF secretion by human mesenchymal stem cells, Cellular and Molecular Bioengineering, Vol. 6 (2), pp. 220-229, 2013.
T. Hatta, H. Sano, N. Sakamoto, K.N. Kishimoto, M. Sato, E. Itoi: Nicotine reduced MMP-9 expression in the primary porcine tenocytes exposed to cyclic stretch. Journal of Orthopaedic Research, Vol. 31 (4), pp. 645-650, 2013.
M. Sato, S. Kawamoto, M. Watanabe, N. Sakamoto, M. Sato, Y. Tabata, Y. Saiki: Medical Regeneration using a Biodegradable Felt as a Scaffold Preserves Integrity and Compliance of a Canine Dissected Aorta. Circulation, Vol. 126, pp. S102-S109, 2012.
T. Anno, N. Sakamoto, M. Sato: Role of Nesprin-1 in Nuclear Deformation in Endothelial Cells under Static and Uniaxial Stretching Conditions. Biochemical and Biophysical Research Communications, Vol. 424 (1), pp. 94-99, 2012.
L. Yuan, N. Sakamoto, G. Song, M. Sato: Migration of human mesenchymal stem cells under low shear stress mediated by MAPK signaling. Stem Cells and Development, Vol. 21 (13), pp. 2520-2530, 2012.
W. Huang, N. Sakamoto, M. R. Miyazawa, M. Sato: Role of paxillin in the early phase of orientation of the vascular endothelial cells exposed to cyclic stretching. Biochemical and Biophysical Research Communications, Vol. 418 (4), pp. 708-713, 2012.
W. Huang, N. Sakamoto, M. Sato: Role of intercellular junctions in redistribution of focal adhesions and orientation of vascular endothelial cells exposed to cyclic stretching. Cellular and Molecular Bioengineering, Vol 4 (3), pp. 368-378, 2011.
N. Sakamoto, T. Kiuchi, M. Sato: Development of an Endothelial-Smooth Muscle Cell Coculture Model Using Phenotype-Controlled Smooth Muscle Cells. Annals of Biomedical Engineering, Vol. 39 (11), pp. 2750-2758, 2011.
K. Oya, N. Sakamoto, T. Ohashi, M. Sato: Combined stimulation with cyclic stretching and hypoxia increases production of matrix metalloproteinase-9 and cytokines by macrophages. Biochemical and Biophysical Research Communications, Vol. 412, pp. 678-682, 2011.
K. Nishio, Y. Ueki, N. Sakamoto, M. Sato: Effect of initial orientation of vascular endothelial cells on activation of RhoGTPases induced by fluid shear stress. Cellular and Molecular Bioengineering, Vol. 4 (2), pp. 160-168, 2011.
R. Ichinose, H. Sano, K. Kishimoto, N. Sakamoto, M. Sato, E. Itoi: Alteration of the material properties of the normal supra-spinatus tendon by nicotine treatment in a rat model, Acta Orthopaedica, Vol. 81 (5), 634-638, 2010.
N. Sakamoto, K. Segawa, M. Kanzaki, T. Ohashi, M. Sato: Role of p120-Catenin in the Morphological Changes of Endothelial Cells Exposed to Fluid Shear Stress. Biochemical and Biophysical Research Communications, Vol. 398, pp. 426-432, 2010.
Y. Ueki, N. Sakamoto, M. Sato: Cyclic force applied to FAs induces actin recruitment depending on the dynamic loading pattern. The Open Biomedical Engineering Journal, Vol. 4, pp. 129-134, 2010.
Y. Ueki, Y. Uda, N. Sakamoto, M. Sato: Measurements of strain on single stress fibers in living endothelial cells induced by fluid shear stress. Biochemical and Biophysical Research Communications, Vol. 395, pp. 441-446, 2010.
N. Sakamoto, N, Saito, X. Han, T. Ohashi, M. Sato: Effect of spatial gradient in fluid shear stress on morphological changes in endothelial cells in response to flow. Biochemical and Biophysical Research Communications, Vol. 395, pp. 264-269, 2010.
Y. Ueki, N. Sakamoto, M. Sato: Direct measurement of shear strain in adherent vascular endothelial cells exposed to fluid shear stress. Biochemical and Biophysical Research Communications, Vol. 394, pp. 94-99, 2010.
S. Sugita, T. Murase, N. Sakamoto, T. Ohashi. M. Sato: Size sorting of kinesin-driven microtubules with topographical grooves on a chip. Lab on a Chip, Vol. 10, pp. 755-761, 2010.
H. Fujiwara, Y. Saiki, M. Sato, N. Sakamoto, T. Ohashi, M. Sato, Y. Tabata, K. Tabayashi: Modification of the descending thoracic aortic anastomotic site using biodegradable felt: Study in a canine model with or without basic fibroblast growth factor. Journal of Vascular Surgery, Vol. 51, pp. 194-202, 2010.
T.S. Matsui, S. Deguchi, N. Sakamoto, T. Ohashi, M. Sato: A versatile micro-mechanical tester for actin stress fibers isolated from cells. Biorheology. Vol. 46, pp. 401-415, 2009.
S. Sugita, N. Sakamoto, T. Ohashi, M. Sato: Novel image analysis for trajectory of microtubules gliding on kinesins with tip detection. Journal of Biomechanical Science and Engineering, Vol. 4 (3), pp. 404-414, 2009.
Y. Ueki, N. Sakamoto, T. Ohashi, M. Sato: Morphological responses of vascular endothelial cells induced by local stretch transmitted through intercellular junctions. Experimental Mechanics, Vol. 49, pp. 125-134, 2009.
S. Sugita, N. Sakamoto, T. Ohashi, M. Sato: Characterization of Motility Properties of Kinesin-Driven Microtubules Towards Nano-Scale Transporter: Focusing on Length of Microtubules and Kinesin Density. Journal of Biomechanical Science and Engineering, Vol. 3(4), pp.510-519, 2008.
Ohashi, K. Hanamura, D. Azuma, N. Sakamoto, M. Sato: Remodeling of endothelial cell nucleus exposed to three different mechanical stimuli. Journal of Biomechanical Science and Engineering, Vol. 3 (2), pp. 63-74, 2008.
N. Sakamoto, Y. Yamazaki, T. Ohashi, M. Sato: Effect of mechanical environment of focal adhesions on remodeling of endothelial cells subjected to cyclic stretching using microsubstrates. Journal of Robotics and Mechatronics. Vol. 19, No. 5, pp. 557-564, 2007.
S. Masuda, Y. Saiki, S. Kawatsu, I. Yoshioka, H. Fujiwara, S. Kawamoto, S. Sai, A. Iguchi, N. Sakamoto, T. Ohashi, M. Sato, K. Tabayashi: Trail of New Vascular Clips for Aortic Anastomosis in a Canine Model. The Journal of Thoracic and Cardiovascular Surgery, Vol. 134, No. 3, pp. 723-730, 2007.
T. Ohashi, Y. Sugaya, N. Sakamoto, M. Sato: Hydrostatic pressure influences morphology and expression of VE-cadherin of vascular endothelial cells. Journal of Biomechanics, Vol. 40 (11), pp. 2399-2405, 2007.
K. Ito, N. Sakamoto, T. Ohashi, M. Sato: Effects of Frequency of Pulsatile Flow on Morphology and Integrin Expression of Vascular Endothelial. Technology and Health Care, Vol. 15, pp. 91-101, 2007.
大橋俊朗,瀬川圭,坂元尚哉,佐藤正明:HUVECの形態およびVEカドヘリン発現に与える静水圧の影響.生体医工学,Vol. 44,pp. 454-459,2006.
N. Sakamoto, M. Oi, T. Ohashi, M. Sato: Nitric oxide production and smooth muscle cell phenotype of endothelial cell-smooth muscle cell cocultured model. Journal of Biomechanical Science and Engineering. Vol. 1, pp. 224-233, 2006.
G. Song, T. Ohashi, N. Sakamoto, M. Sato: Adhesive force of human hepatoma HepG2 cells to endothelial cells and expression of E-selectin. Molecular and Cellular Biomechanics. Vol. 3 (2), pp. 61-68, 2006.
N. Sakamoto, T. Ohashi, M. Sato: Effect of fluid shear stress on migration of vascular smooth muscle cells in cocultured model. Annals of Biomedical Engineering, Vol. 34 (3), pp. 408-415, 2006.
H. Fujiwara, K. Oda, Y. Saiki, I. Yoshioka, S. Kawatsu, N. Sakamoto, T. Ohashi, M. Sato, Y. Tabata, K. Tabayashi: The wrapping method using biodegradable felt strips has a preventive effect on the thinning of the aortic wall: Experimental study in the canine aorta. Journal of Vascular Surgery, Vol. 43 (2), pp. 349-356, 2006.
N. Sakamoto, T. Ohashi, and M. Sato: Effect of shear stress on permeability of endothelial monolayer cocultured with smooth muscle cells. JSME International Journal, Series C, Vol. 47(4), pp. 992-999, 2004.
Y. Sugaya, N. Sakamoto, T. Ohashi and M. Sato: Elongation and random orientation of bovine endothelial cells in response to hydrostatic pressure: comparison with response to shear stress. JSME International Journal, Series C, Vol. 46(4), pp. 1248-1255, 2003.
T. Ohashi, N. Sakamoto, A. Iwao and M. Sato: Oxygen tension modulates Ca2+ response to flow stimulus in endothelial cells exposed to hydrostatic pressure. Technology and Health Care Vol. 11, pp. 263-274, 2003.
佐藤正明,武田浩一郎,片岡則之,坂元尚哉:培養動脈組織片の初期ひずみ状態が内皮細胞の形態および細胞骨格構造に及ぼす影響.日本機械学会論文集(A編),Vol. 69(677),pp. 30−36,2003.
N. Sakamoto, T. Ohashi, M. Sato: Effect of magnetic field on nitric oxide synthesis of cultured endothelial cells. International Journal Applied Electromagnetics and Mechanics, Vol. 14, pp. 317-322, 2001/2002.
佐藤正明,坂元尚哉,出口真次,加藤みゆき,阿部皓一:ビタミンEの白血球粘着抑制効果および血小板凝集抑制作用.医学と薬学.Vol. 47(3), pp. 457-461、2002.
武田浩一郎,片岡則之,坂元尚哉,佐藤正明:培養動脈組織片を用いた内皮細胞の流れに対する形態的反応の検討.医用電子と生体工学,Vol. 36(3),pp. 293-301、1998.
PROCEEDEINGS
Y. Lin, N. Sakamoto, G. Song, M. Sato: Low Shear Stress Enhances Human Mesenchymal Stem Cell Migration through MAPK Activity. Stem Cell Programming & Reprogramming, 2011 (in press).
R. Miyazawa, W. Huang, N. Sakamoto, M. Sato: Role of Paxillin in Orientation of Vascular Endothelial Cells Exposed to Cyclic Stretch. The 5th Wolrd Congress on Bioengineering, in CD, 2011.
W. Huang, N. Sakamoto, K. Hanamura, R. Miyazawa, M. Sato: Intercellular Junctions Play an Important Role in Orientation of Vascular Endothelial Cells Exposed to Cyclic Stretch. ASME 2011 Summer Bioengineering Conference, in CD, 2011.
M. Sato, N. Saito, N. Sakamoto, T. Ohashi: High Wall Shear Stress Gradient Suppress Morphological Responses of Endothelial Cells to Fluid Flow. World Congress on Medical Physics and Biomedical Engineering, in CD, 2009.
N. Sakamoto, T. Ohashi, M. Sato: Influence of fluid shear stress on matrix metalloproteinase production in endothelial cells. The 13th International Conference on Biomedical Engineering, in CD, 2008.
N. Sakamoto, Y. Ueki, T. Ohashi, M. Sato: Fluid shear stress suppresses transendothelial migration of leukocytes in coculture model. NUS-Tohoku Graduate Student Conference in Bioengineering, in CD, 2008. (Invited)
N. Sakamoto, T. Ohashi, M. Sato: High Shear Stress Induces Production Of Matrix Metalloproteinase In Endothelial Cells. Proceedings of the 2008 Summer Bioengineering Conference, in CD, 2008.
D.W. Chew, N. Sakamoto, M. Sakamoto, L.B. Sanders, M.S. Makaroun, D.A. Vorp: Association of hypoxia with impaired remodeling mechanisms in the abdominal aortic aneurysm (AAA) wall. The FASEB Journal, 22, 174.22, 2008.
T. Matsui, S. Deguchi, N. Sakamoto, T. Ohashi, M. Sato: Stress relaxation properties of stress fibers isolated from smooth muscle cells. The 9th International Symposium on Future Medical Engineering Based on Bio-nanotechnology, 66-67, 2007.
K. Ito, N. Sakamoto, T. Ohashi, M. Sato: FRAP analysis of actin dynamics of smooth muscle cells in response to local mechanical stimulation. The 9th International Symposium on Future Medical Engineering Based on Bio-nanotechnology, 68-69, 2007.
N. Sakamoto, T. Ohashi, M. Sato: Effect of fluid shear stress on smooth muscle cell migration and permeability of endothelial monolayer in endothelial-smooth muscle cells cocultured model. Proceedings of the Final Symposium of the Tohoku University 21st Century Center of Excellence Program , 163-172, 2007.
K. Baria, Y. Sugaya, N. Sakamoto, T. Ohashi, M. Sato: Hydrostatic pressure influences sub-cellular localization of b-catenin of vascular endothelial cells. Proceedings of the Final Symposium of the Tohoku University 21st Century Center of Excellence Program, 61-70, 2007.
K. Ito, N. Sakamoto, T. Ohashi, M. Sato: Frequency of shear stress modulates morphology and integrin expression of vascular endothelial cells. Proceedings of the Final Symposium of the Tohoku University 21st Century Center of Excellence Program, 103-110, 2007.
D.A. Vorp, D.W. Chew, N. Sakamoto, M. Sakamoto, M.S. Makaroun: Association of biophysical forces with impaired remodeling mechanisms of the abdominal aortic aneurysm wall. Journal of Biomechanics, Vol. 39, Supplement 1, pp. S289-S290, 2006.
N. Sakamoto, M. Oi, Y. Ueki, T. Ohashi, M. Sato: Adhesive property of leukocytes to endothelial cells in cocultured model exposed to fluid shear stress. Proceedings of the 2005 Summer Bioengineering Conference, in CD, 2005.
N. Sakamoto, T. Ohashi, and M. Sato: Endothelial cells exposed to fluid shear stress regulate migration of cocultured smooth muscle cells. Proceedings of the 2003 Summer Bioengineering Conference, 621-622, 2003.
N. Sakamoto, T. Ohashi, and M. Sato: Fluid shear stress affects migration of vascular smooth muscle cells cocultured with endothelial cells. Proceedings of International Congress on Biological and Medical Engineering in Singapore, 240pdf in CD, 2002.
N. Sakamoto, T. Ohashi, and M. Sato: Oscillating magnetic field affects NO synthesis of cultured endothelial cell. Proceedings of 10th International Conference on Biomedical Engineering. 433-434, 2000.
K. Takeda, N. Kataoka, N. Sakamoto, M. Sato: Cytoskeletal change of endothelial cells of cultured aortic segment exposed to shear stress. 4th International Confercnce on Cellular Engineering, P-17, 1999.
M. Sato, N. Sakamoto, K. Takeda, and N. Kataoka: Endothelial cell responses to fluid shear stress using excised aortic segments. Proceedings of the 3rd International Conference on Cellular Engineering, 51-52, 1997.