MICE: Jax (Mice, Phenome, Genome), NCI, Charles River Labs
CELLS:
Primary cells: ScienCell, Invitrogen
Human or Nonhuman Primate: NIH NHP/UMass (Non-primate reagents, HSC-F cyno T cell line), Nat. Primate Res Ctr/U.Wisconsin, ProteoGenex, Bioreclamation, Cybrdi, AllCells
Isolation: Invitrogen Dynabeads, Miltenyi
Lymphocyte separation: GE Healthcare (Ficoll-Paque Plus), Sigma-Aldrich (Accuspin tubes), Axis-Shield (Lymphoprep tubes), Greiner Bio-One (Leucosep)
Nucleic Acids: OriGen (siRNA, cDNA), GenScript (seq synthesis), Biosynthesis (primers), Promega
PROTEINS: R&D Systems (Technical > Unit conversion), PeproTech Inc. (cytokines), HumanZyme, KPL
MAbs: Biocompare, Linscott's directory, Abpedia, BD, R&D (conversion), Bio X Cell, eBioscience, GenWay, BioLegend, LifeTech, Jackson, Miltenyi (MACS), AbD (x)Serotech, ABCam, Upsate, Quiagen, ABR, Acris(Gmy), GeneTex, Millipore, SoutherBiotech, Abs Inc, LSBio, Santa Cruz, Sigma-Aldrich, Covance (pAb maker)
F(ab')2: FragIT Purification Kit (Bulldog Bio), F(ab')2 prep kit (Thermo Pierce)
AB LABELING: Mol Probes (Alexa488), Pierce (biotin, HRP), KPL, Qiagen
Microbeads: Dynal (M270/280/MyOne streptavidin, M280/450 Tosylactivated), MACS(3.5μM)
MEDIA:
Life Sci: Serum-free: Gibco/Invitrogen, RPMI-1640 (formulation 22400 )
Serum, IgG, albumin: Jackson, Valley Biomedical
CHEMICALS: Sigma-Aldrich (Acids & bases), EMD (Calbiochem), Molecular Probes/Invitrogen
ELISA: R&D
ELISArray: MSD (spot maps), Qiagen, Quansys, Invitrogen
Other reagents:
Chlodronate liposome: Encapsula Nano (Products), Dutch
Plastics:
Nunc, Corning Costar
Low-absorption:
Corning Costar ultra-low attachment plates, Untreated V-shaped plate (3896), Nunc untreated conical bottom (249935, 52)
Bio Plas Inc: siliconized 0.5ml tubes (4200SLS)
Millipore, CDC Tech, Fine Science Tools, Hamilton, Roche,
EQUIPMENT: Thermo, Corporate Exp, Office Depot, Brady (Labxpert labeling system)
Invitrogen/Gibco: 2-ME (21985-023), ACK (006-0005DG), 0.5M EDTA pH8 (15575-038)
BD Biosciences: Matrigel (354234)
Commonly used solution: Invitrogen,
colorpHast* (EM Science), Western stripping (Pierce #21059)