Culture of immune cells
Macrophages ind: monocytes + 100 ng/ml M-CSF for over 5 days
T-cell culture: Wiley's Current Protocols, Current Protocols in Immunol
Hemacytometer: Average # of cells on a large square x dilution factor x 104 (cells/ml)
CFSE labeling: CellTrace CFSE cell prol kit (Mol Probe, C34554); Sci-Mate;
Analyze ELISA data
GraphPad Prism: ProtocolPlace, Top Tip Bio, Cisbio
Excel: MyAssays, Mark Garcia
Flow cytomtry: Spectra Viewer
Ab labeling kits
Invitrogen (Molecular Probes), Signa-Aldrich, Pierce, PromoKine
Alexa Fluor 488 labeling kit (Invitrogen Molecular Probes, #A20181), Dialysis cassettes (Pierce);
Biotinylation: Thermo/Pierce, CampellSci
Primer, Alignment: Blast, Primer3+, Vector NTI, DNAStar, ClustalW2, SIB Blast
Reagent preparation:
How to dissolve peptides: Life Protein, GenScript
ug/ml (* uMole/MW ug) = uMole/mL = micro-/milli- x Mole/L = mM = 106 nM
Acid concentrations: OpenWetWare,
Centrifugal force: RCF (xg) = 1.118 * 10-5 * Radius * RPM2, Beckman Coulter, Kubota
Cell counting:
Cell counter: # in a large square x 104/ml x dilutions
Countess Cell Counter: Live/dead #'s/ml = Reading * (dilution folds / 2)
Uneven serial dilutions:
Target ingredient conc. = 1/first * 1/sec * ... * 1/nth = 1/final
Yotta (1024), Zetta (1021), Exa (1018), Peta (1015), Tera (1012), Giga (109), Mega (106), Kilo (103), Hecto (102), Deca (101)
One (100)
Deci (10-1), Centi (10-2), Milli (10-3), Micro, μ (10-6), Nano (10-9), Pico (10-12), Femto (10-15), Atto (10-18), Zepto (10-21), Yocto (10-24)
Number prefix: Wiki