pE-4000 (CoolLED)
pE-4000 (CoolLED)
NEW GENERATION LEDs The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy.
The system has 16 selectable LED wavelengths across 4 channels that can be matched to the filters and fluorophores of almost any microscope, making it the broadest spectrum of illumination available.
The CoolLED pE-4000 now benefits from our award winning sustainable Green technology and delivers enhanced intensity at your microscope sample plane with a significant reduction in energy consumption, and is supplied with a 3 year warranty after registration.
The NEW Enhanced pE-4000 is a powerful 4-channel high-specification LED illumination system. It is flexible, controllable and environmentally friendly. Benefits include:
SELECTABILITY
Choice of 256 wavelength combinations from 16 installed LEDs (365nm -770nm)
Choose 1 of 4 wavelengths for each 4 channels on the control pod
Unique wavelength grouping concept (see below)
Optimum wavelength to suit your experiment
Specific wavelength characterisation for optimal optogenetic control of cell responses
Wavelength Grouping Concept
As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains. These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.
DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above.
CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available. This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.
With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets. The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.
The pE-4000 allows multiple experiments with varying wavelengths on the same sample , as shown here.
(A) Representative photocurrent traces of a PhobosCA expressing CA1 cell evoked with different activation wavelengths and shutoff with 595nm light
(B) Photocurrent traces in the same cell evoked with 460nm light and shutoff with indicated wavelengths (10mW/mm2)
Wietek et al (2017) Anion-conducting channelrhodopsins with tuned spectra and modified kinetics engineered for optogenetic manipulation of behaviour, Scientific Reports volume 7, Article number 14957(2017) doi: 10.1038/s41598-017-14330-y
SUSTAINABILITY
Lowest Power Consumption – other LED technology uses 120-350 Watts
>25000 hours operation
Highly stable, repeatable LED technology
Market leading energy efficiency
CELL VIABILITY
Optimal illumination through control features to extend fluorescence of cells
Reduced photobleaching and phototoxicity
Microsecond switching
Variable pulse duration on/off
“When you can only control intensity of ‘white’ light (rather than individual channels), the level of photobleaching can be high. With the pE-4000, we can control the excitation of the individual channels. It is possible to optimise the excitation intensity according to the labelling, greatly reducing photobleaching and phototoxicity in a live experiment.”
Dr Yan Gu, University of Sussex
“Suddenly, we were able to offer users uninterrupted extended live cell experiments of 100+ hours, without worrying about brightness fluctuations, lamps burning, room heating, etc. Also, users have reported markedly reduced bleaching and phototoxicity in their samples, both from the prokaryotic and the eukaryotic research fields.”
Dr Jens Eriksson, Oslo University Hospital
CONTROLLABILITY
Advance integration into major microscope software platforms
Save wavelength combinations and return to previous set-up
USB interface
Sequence runner
High speed TTL Triggering
Analogue control – dynamic intensity sinusoidal control option
White light control
Expansion Box
Individual channel intensity control
Pre-determined intensity saving option
VISIBILITY
High signal to noise ratio which gives cleaner images and data
“Striking, bright fluorescence images with a strong signal to noise ratio even when using a low magnification objective”
Graham Wright, Institute of Medical Biology, A*STAR Singapore
Time series of a confluent sheet of keratinocytes migrating during a live-cell imaging outgrowth assay, acquired on an Olympus IX-83 microscope equipped with a CoolLED pE-4000 lightsource (Fig. 4). Scale bar = 150 μm
ADAPTABILITY
Fits to all major research and clinical microscopes
Delivers light through a liquid light guide or fiber
Offers inline removable excitation filter holders
Uses the Universal Collimator
RELIABILITY
Assured through experience and QA assured processes
3 year Warranty after registration
First year Warranty swap
The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics. A dual mode control pod gives manual control in either White or Advanced modes. Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.
The pE-4000 offers:-
Broadest LED spectrum available: 365-770nm – match LED peaks to existing microscope filters
Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters
Individual control of selected LED wavelengths – less background, no filter wheel or shutters
Integrated with many imaging software packages - can be incorporated into all experimental parameters
Instant on/off – no shutters required, no warm up or cool down
Stable & repeatable – reliable and consistent results
Wide range of Microscope Adaptors – fits most microscopes
Precise intensity control in 1% steps (0-100%) – no ND filters required
Excellent uniformity over field of view – fixed and stable, no alignment necessary
Long lifetime – expected to exceed 25,000 hours of operating time
IDEAL FOR MULTI-USER AND CORE FACILITIES
Pre-sets allowing lab manager to match white spectrum to existing filter cubes
Higher contrast images from matched-white spectrum
Simple to fit, simple to use
No training required
IDEAL FOR ADVANCED RESEARCH
Individual LED wavelength selection
Rapid switching between LED wavelengths enables capture of high speed events
TTL & USB interfaces with imaging packages
Excitation filters can be fitted in optical path for controlled switching with no moving parts
Analogue inputs for dynamic intensity control
Optical feedback for applications requiring higher stability
Internal function generator for electrophysiology and optogenetics applications
Power requirements
110-240V a.c. 50/60Hz, 2.5A
Power consumption
Standby (i.e. no LEDs on) : Max 7W
Single wavelength operation : Max 25W
Dual wavelength operation : Max 44W
Triple wavelength operation : Max 53W
Quad wavelength operation : Max 60W
Dimensions
pE-4000 Light Source: 150mm(w) x 220mm(d) x 260mm(h) - Weight 3.5kg
pE-4000 Control pod: 154mm(w) x 135mm(d) x 40mm(h) – Weight 0.95kg
pE-4000 Power Supply: 164mm(w) x 64mm(d) x 35mm(h) – Weight 0.58kg
pE-Expansion Box: 151mm(w) x 18mm(d) x 95mm(h) - Weight 0.34kg
Environment & Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:
Mercury-free & Laser-free
Energy Efficient
Long lifetime (25,000 operating hours)
No bulb replacements
Reduced risk of eye damage
Quiet operation
No special disposal regulations or issues
Warranty
System =24 months extendable by free product registration. LEDs = 36 months
Manual : Dual function remote manual control pod for White mode or Advanced mode
Remote: Via USB for independent on/off and intensity control of each channel. Triggering speed approx 1ms
Via 4 TTL inputs for independent on/off control of each channel. Triggering speed <20us
Via single TTL for on/off control of manual or software selected channels
Via 4 analogue inputs 0-10V, 0-300kHz for dynamic control of intensity from external analogue signals
Synch Out: 4 TTL outputs for each channel – active high 1 TTL output for any channel – active high
Programmable interface: 4 TTL outputs for on/off control of peripherals (transmitted illuminators, stages etc.) 4 analogue outputs for intensity control of peripherals (can be programmed to mirror LED intensities for channel control) 0-10V full scale.
Function Generator: Internally generated sine, pulse and ramps for each channel programmed via control pod.
Connectivity: USB (B type) for PC connection. All other TTL and Analogue inputs/outputs via 25way 'D-type' female connector (optional rear mounting pE-Expansion Box available for BNC connectivity).
Imaging Software: Recognised under common software e.g. Micromanager, MetaMorph, cellSens, NIS Elements, etc. see Imaging Software
Ordering
pE-4000-L-SYS-ZZ: pE-4000 Light Source with manual control pod, and power supply for LLG delivery
pE-4000-F-SYS-ZZ: pE-4000 Light Source with manual control pod and power supply for Fiber delivery
pE-4000-EB25D: Rear mounting pE-Expansion Box for 25-way D-type to BNC connectivity
pE-4000-EFH-4 Set of four Excitation Filter holders (25mm dia.)
pE-1906: 1.5m long, 3mm diameter liquid light guide
pE-10400: pE-Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY Microscope Adaptor to customer-specified microscope
A range of fibers is available from CoolLED, go to Accessories tab To specify microscope code (YYY), see Adaptors
To specify Power Cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA