pE-340fura (CoolLED)

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The images above show a field of cardiac myocytes (heart cells). The cells were loaded with Fura-2 using standard conditions (i.e. incubation with 2 micromolar Fura-2 acetoxymethyl ester for 30 minutes, followed by an additional 30 minutes for de-esterification.

Images were obtained by Martin Bootman and Katja Rietdorf, School of Life, Health and Chemical Sciences, The Open University, UK.

Until recently, the response time of illumination systems for Fura-2 imaging have been limited to milliseconds due to mechanical switching of the wavelengths in arc lamp and monochromator systems. However, the new pE-340^fura can be controlled via convenient BNC TTL connections for precise illumination control in as little as 20 microseconds.

Spontaneous Ca²⁺ events are induced in Mg²⁺-free HBS. (A) Representative trace from a single hippocampal neuron of Mg²⁺-free induced Ca²⁺ events imaged at 0.5 Hz and (B) representative trace from two hippocampal neurons of Mg²⁺-free induced Ca²⁺ events imaged at 24.39 Hz.

TINNING, P. W., FRANSSEN, A. J. P.M., HRIDI, S. U., BUSHELL, T. J. and MCCONNELL, G. (2017), A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca²⁺ imaging of live cells with better than 5 nM precision. Journal of Microscopy. doi:10.1111/jmi.12616

Using the new pE-340^fura LED Illumination System, less Fura-2 dye can be loaded into the cells whilst still maintaining the same measured calcium concentration and good signal-to-noise ratio. The reduction in required dye not only improves cell-viability due to reduced dye toxicity, but also results in a cost reduction per experiment.

Comparison of Ca²⁺ increases obtained from the application of trypsin (100 nM) to tsA-201 cells loaded with different concentrations of Fura-2 AM.

TINNING, P. W., FRANSSEN, A. J. P.M., HRIDI, S. U., BUSHELL, T. J. and MCCONNELL, G. (2017), A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca²⁺ imaging of live cells with better than 5 nM precision. Journal of Microscopy. doi:10.1111/jmi.12616

Work by Sandrine Prost et al., from the University of Edinburgh, has shown that with independent wavelength controllable LED sources, signal-to-noise is dramatically improved over bulb systems and even over some available white wide spectrum LED sources.

Prost S et al (2016) Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections. PLoS ONE 11(9): e0162419. doi:10.1371/journal.pone.0162419

There are two pE-340^fura configuration options:

Direct-fit for connecting to a microscopes – by selecting from a range of microscope adaptors which covers all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide with a fixed 3mm diameter, liquid light guide. An optional pE-340^fura Universal Collimator can be specified in conjunction with a microscope adaptor if required, containing optics optimised to transmit the 340nm.

Power requirements

100-240V a.c. 50/60Hz, 1.4A

Power consumption

Standby Max 2W

1 band (white) at 100% intensity Max 24W

2 bands (340 + white) at 100% intensity Max 30W

3 bands (All) at 100% intensity 36W

Dimensions

pE-340^fura Light Source: 77mm(w) x 186mm(d) x 162mm(h) - Weight 1.40kg

pE-340^fura Control Pod: 88mm(w) x 125mm(d) x 37mm(h) - Weight 0.32kg

pE-340^fura Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg

pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg

Environment & Safety

LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:

• Mercury-free

• Energy Efficient: 80% less power

• Long lifetime (25,000 operating hours)

• No bulb replacements

• Reduced risk of eye damage

• Quiet operation

• No special disposal regulations or issues

Warranty

System = 24 months extendable by free product registration.

LEDs = 36 months (NB 340nm LED warranted for 3000 hours accumulated use)

Control pod for operation and access to settings and usage history.

Manual:

Manual control for instant on/off and intensity control in 1% steps from 0 – 100%

Individual bands can be selected/deselected and controlled independently as desired

Remote:

Via global and individual channel TTL for on/off control of selected channels using a BNC connection on the light source.

Triggering speed <20us at full power

Connectivity:

Remote via USB (B type) for imaging software control (see Imaging Software)

Sequence Runner:

Single TTL input to step through user defined sequence.

Triggering speed <20us at full power

Ordering

pE-340-FR-D-YYY-ZZ:

pE-340^fura Illumination System. Direct Fit. Includes Light Source, exchangeable microscope adaptor (YYY) to customer-specified microscope, remote manual control pod, set of three excitation filter holders (25mm dia.), Excitation Filters for 340nm & 380nm, and power supply (ZZ).

pE-340-FR-L-ZZ:

pE-340^fura Illumination System. For use with 3mm Liquid Light Guide. Includes Light Source, remote manual control pod, set of three excitation filter holders (25mm dia.), Excitation Filters for 340nm & 380nm, and power supply (ZZ).

pE-1910

3m long, 3mm diameter UV liquid light guide for use with pE-340^fura

pE-340-FR-COLL-YYY

pE-340^fura Universal Collimator for use with a single liquid light guide. Includes Microscope Adaptor (YYY) to customer-specified microscope

To specify microscope code (YYY) see Adaptors

To specify power cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA

Reviews

can absolutely recommend this light source for any kind of Fura-2 measurements.

Lars Kaestner and Quinghai Tian, Molecular Cell Biology, University of Saarland, Germany