Exact BR / Exact FL
(Axion/CytoSmart)
홈 / 제품소개 / 현미경 / 셀 카운터 (Cell Counter) / Exact
Exact
Cell counting is an essential step in many experimental, clinical, and diagnostic workflows. The Exact FL is an automated cell counting platform designed for efficient and accurate assessment of cell concentrations and viability. The state-of-the-art optics and image analysis software offers an unprecedented level of detail, allowing it to distinguish between cells and debris, detect individual cells within a cluster, or count organoids and measure their size.
Key Features
>> Count faster with less user variability – Manual cell counting is time-consuming and laborious. The Exact FL uses innovative machine-learning software coupled with an unmatched resolution and expanded field of view, minimizing user-to-user variability and increasing reproducibility, so you can focus on your experiment.
>> Get more with fluorescence – In addition to brightfield cell number and viability, red and green fluorescence channels let you count specific populations within your sample.
>> Plug it in and get started – With an easy-to-use, maintenance-free device that does not require calibration, any lab can quickly start using the Exact FL.
Overview
The Exact FL is an efficient and affordable cell counting platform. Both brightfield and fluorescence-based cell counting combined with powerful CMOS-based optics and AI-driven analysis takes the guesswork out of routine cell counting and provides accurate and reproducible results.
Software/Algorithms
The Exact FL is available with two software modules to match your assay needs:
Cell counting – Available in brightfield and fluorescence. Accurately measure cell concentration, size, and viability for cell seeding, protocol standardization, cell growth assessment, and more.
Organoid Counting – Count and analyze organoids using an advanced machine-learning algorithm.
The Organoid Counting module for the Exact platform can detect organoids and spheroids of various shapes and sizes. In addition to counting the total number of organoids, the module can quantify organoid size and distribution. With the latest in image analysis algorithms, quickly and accurately count your organoids and spheroids.
FAQ:
Can the Exact FL assess cell viability?
Yes, cell viability can be assessed either using green/red fluorescence channels (AO/PI) or a brightfield channel (Trypan Blue). In the Exact FL, when the green and red fluorescence channels are deselected, the Trypan Blue option will appear automatically.
Does the Exact FL work with any cell counting chamber?
Yes, as long as the counting chamber is transparent and has a depth of 0.1 mm. It does not matter if the cell counting chamber is made of glass, plastic, has a counting grid or if there are multiple counting chambers.
Is a light cover required for fluorescence cell counting?
To get the best results, we advise covering the stage of the device with the provided adapter plate to minimize the amount of environmental light.
Is a computer required?
The Exact FL requires a desktop or laptop running on Windows 10 or above, with a USB3 port and an active internet connection. A WiFi or Ethernet connection is necessary for connection to the Axion Portal for image analysis and storage.
How do I clean the Exact FL?
The devices are easy to clean using lint-free wipes and ethanol (70%) or isopropyl alcohol (IPA). Do not use acetone to clean the device, also the device cannot be autoclaved.
Organoid Counting Module
The Organoid Counting module for the Exact platform can detect organoids and spheroids of various shapes and sizes. In addition to counting the total number of organoids, the module can quantify organoid size and distribution. With the latest in image analysis algorithms, quickly and accurately count your organoids and spheroids.
Key Features
>> Accurate – Consistently count the number of organoids in your sample.
>> Fast – Minimize user-to-user variability with advanced image analysis that incorporates innovative machine-learning algorithms.
>> Powerful – Get better data with software that assesses the number of organoids or spheroids in a sample.
Overview
With the Organoid Counting Module:
>> Quantify organoids and spheroids automatically
>> Measure organoid size and distribution
The Organoid Counting Module enables robust and accurate quantification of organoids and spheroids in a sample. The module can also assess the size and population distribution of organoids and spheroids, making it an excellent tool for studying and optimization of 3D cell models.
Metrics provided by the Organoid Counting Module:
>> Organoid concentration (organoids/mL) - average concentration and concentration of individual counts
>> Organoid area (µm2) - Organoid area