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Post date: May 17, 2013 2:26:08 PM
September 9, 2009
Dr. Keirstead's current stem cell research utilizes federally-approved and non-federally-approved hESC lines. He developed a method to differentiate hESC lines into high purity oligodendrocyte progenitor populations, demonstrating for the first time that hESC lines can be differentiated into high purity CNS populations, and making available for the first time high purity human oligodendroglial lineage cells for research and therapy. He is currently investigating points of control in the maturation of stem cells to various CNS lineages besides oligodendrocytes. He has also recently demonstrated the regenerative ability of these cells in animal models of spinal cord injury and multiple sclerosis, where they remyelinate and facilitate functional locomotor recovery. He transplanted these cells into acute and chronic spinal cord injured adult rats, and rodent models of multiple sclerosis, to investigate their ability
to survived, differentiate into myelinating oligodendrocytes, and restore locomotor ability. In this manner he is comparing the regenerative efficacy of different hESC lines, as well as young and old passage hESC lines. He is also investigating the ability of transplanted hESC derivatives to migrate in the adult CNS following manipulation of the environment, including exercise of animals. Finally, he is conducting gene chip and other molecular analyses of hESCs and their derivatives to determine points of control during their differentiation, and the effects on hESC derivatives following exposure to the injured CNS.
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