Publications

Selected publications, h factor 34: ORCID iD: 0000-0002-48922739 

2023

• Dubern, J-F., Halliday, N., Camara, M., Winzer, k., Barrett, D.A., Hardie, K.R., and Williams, P. (2023) Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa. Microbiology. 10.1099/mic.0.001316

• Kasza, K., Elsherbeny, A., Moloney, C., Hardie, K.R., Camara, M., Alexander, C., and Gurnani, P. (2023) Hybrid poly(ß-amino ester) triblock copolymers utilising a RAFT polymerisation grafting-from methodology. Macromolecular Chemistry and Physics https://doi.org/10.1002/macp.202300262.

2022

• Hardie, K.R. and Fenn, S.J. (2022) Antimicrobial Profile: Rifampicin: a broad-spectrum antibiotic. In press Journal of Medical Microbiology. 10.1099/jmm.0.001566

2021

• Soh, E. Y-C., Smith, F., Gimenez, M.R., Yan, L., Verjborg, R.M., Fletcher, M., Halliday, N., Bleves, S., Heeb, S., Camara, M., Givskov, M., Hardie, K.R., Tolker-Nielsen, T., Bérengère, I., and Williams, P. (2021) Disruption of the Pseudomonas aeruginosa TAT system perturbs PQS-dependent quorum sensing through lack of the Rieske cytochrome bc1 sub-unit. PLoS Pathogens. https://doi.org/10.1101/2021.03.01.433341 https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009425

• Hollmann, B., Perkins, M., Chauhan, V.M, Aylott, J.W., and Hardie, K.R. (2021) Fluorescent nanosensors reveal dynamic pH gradients duringbBiofilm formation. NPJ Biofilms and Microbiomes. 7, 50 (2021). https://doi.org/10.1038/s41522-021-00221-8https://rdcu.be/cmIic https://biorxiv.org/cgi/content/short/2020.07.31.230474v1

• Armstrong, T., Fenn, S.J., and Hardie, K.R. (2021) Antimicrobial Profile: Carbapenems: a broad-spectrum antibiotic. In press Journal of Medical Microbiology 2021 Dec;70(12). https://doi.org/10.1099/jmm.0.001462

• Kasza, K., Gurnani, P, Hardie, K.R., Miguel Camara, M., and Alexander, C. (2021) Challenges and solutions in polymer drug delivery for bacterial biofilm treatment: a tissue-by-tissue account. Advanced Drug Delivery Reviews vol 178.https://doi.org/10.1016/j.addr.2021.113973

• Armstrong, T., Fenn, S.J., and Hardie, K.R. (2021) Antimicrobial Profile: Carbapenems: a broad-spectrum antibiotic. Journal of Medical Microbiology 2021 Dec;70(12). https://doi.org/10.1099/jmm.0.001462

2020

• Hardie, K.R. (2020) Antimicrobial resistance: the good, the bad and the ugly. Invited Review Emerging Topics in Life Sciences. ETLS20190194. doi: https://doi.org/10.1042/ETLS20190194

• Zhang, J., Brown, J., Scurr, D., Bullen, A., McLellan-Gibson, K., Williams, P.,  Alexander, M.R., Hardie, K.R., Gilmore, I.S., and P. D. Rakowska, P.D. (2020) Label-free 3D molecular imaging of a P. aeruginosa biofilm using cryo-OrbiSIMS. Analytical Chemistry https://doi.org/10.1021/acs.analchem.0c01125.  https://www.biorxiv.org/content/10.1101/859967v1 

• Barzan, G., A. Sacco, A., Mandrile, L., Giovannozzi, A.M., Brown, J., Portesi, C., Alexander, M.,  Williams, P., Hardie, K.R., and Rossi, A.M. (2020) New frontiers against multi-drug resistance: A Raman-based approach for detection of bacterial viability and cross-induced antibiotic resistances within 1 hour. In press Sensors and Actuators B: Chemical. https://doi.org/10.1016/j.snb.2020.127774

• Jordana-Lluch, E., Garcia, V., Kingdon, A., Singh, N., Alexander, C., Williams, P., and Hardie, K.R. (2020).  Development of a polymicrobial model to examine interactions between commensals and pathogens on skin. Frontiers in Microbiology 11:291. doi: 10.3389/fmicb.2020.00291.

2019

• Hardie, K (2019) Where bacterial metabolism and virulence intersect. Microbiology Today. May https://microbiologysociety.org/publication/current-issue/metabolism-health-and-disease/article/where-bacterial-metabolism-and-virulence-intersect.html

•  Singh,N., Romero,M., Travanut, A., Monteiro, P., Jordana-Lluch,E., Hardie, K.R., Williams, P., Alexander M., and Cameron Alexander, C. Dual bioresponsive antibiotic and Quorum Sensing inhibitor Ccmbination nanoparticles for treatment of Pseudomonas aeruginosabiofilms in vitroand ex vivo.  Biomaterials Science. DOI: 10.1039/c9bm00773c

•  Lary, D., Calvert, A., Nerlich, B., Segal, J., Vaughan, N., Randle, J.  and Hardie, K.R. (2019). Improving children’s and their visitors hand hygiene compliance. In Journal of Infection Prevention http://biorxiv.org/cgi/content/short/355818v1doi: https://doi.org/10.1101/355818 https://doi.org/10.1177/1757177419892065

• Brown, J.M., Williams, P., and Hardie, K.R. (2019) Microfluidic-based growth and imaging of bacterial biofilms. Bio-101. https://bio-protocol.org/bio101/about.aspx. https://en.bio-protocol.org/bio101/e3460

2018

• Ashawesh, MM., Markus, R., Penfold, C., and Hardie, KR. Autotransporter secretion exploits the bacterial actin homologue. http://biorxiv.org/cgi/content/short/305441v2.  https://doi.org/10.1101/305441

• Lary, D., Calvert, A., Nerlich, B., Segal, J., Vaughan, N., Randle, J.  and Hardie, K.R. .Improving children’s and their visitors hand hygiene compliance. http://biorxiv.org/cgi/content/short/355818v1doi: https://doi.org/10.1101/355818

2016

• • April 2016: Ruparell A., Dubern JF., Ortori C., Harrison, F., Halliday N., Emtage A., Ashawesh, M., Laughton C., Diggle, SP., Williams P., Barrett D., and Hardie K.R (2016) The fitness burdens imposed by synthesising quorum sensing systems. Nature Scientific Reports. http://www.nature.com/articles/srep33101   http://biorxiv.org/content/early/2016/04/26/050229

2015

• • December 2015: Super Resolution Imaging of immunofluorescent proteins in bacteria featured in The British Pharmacological Society magazine Pharmacology Matters Volume 8, issue 3, p 5-7. https://www.bps.ac.uk/publishing/our-other-publications/pharmacology-matters/pharmacology-matters-library

2014

• • Randle, J., Metcalfe, J., Webb, H., Luckett, JCA, Nerlich, B., Vaughan, N. Segal, J., and Hardie, K.R. (2014) Impact of an educational intervention upon the hand hygiene compliance of children. World Biomedical Frontiers Infection and Immunity. http://biomedfrontiers.org/infection-2014-6-22/

2013

• • Paredes-Osses, E., and Hardie, K.R. (2013) Cell fractionation of Pseudomonas aeruginosa. Bio-protocol http://www.bio-protocol.org/Default.aspx 

  • Soza T. Baban, Sarah A. Kuehne, Amira Barketi-Klai, Stephen T Cartman, Michelle Kelly, Kim R. Hardie, Imad Kansau, Anne Collignon, Nigel P. Minton (2013). The role of flagella in Clostridium difficile pathogenesis: Comparison between a non-epidemic and an epidemic strain. Plos One. 8(9):e73026

  • Randle, J., Metcalfe, J., Webb, H., Luckett, JCA, Nerlich, B., Vaughan, N. Segal, J., and Hardie, K.R. (2013) Impact of an educational intervention upon the hand hygiene compliance of children. Journal of Hospital Infection. 85(3):220-225. IF 3.221

2012

• Luckett, J.C.A, Darch, O., Watters, C., AbuOun, M., Wright, V., Paredes-Osses, E., Ward, J., Goto, H., Heeb, S,, Pommier, S., Rumbaugh, K., Camara, M., and Hardie, K.R. (2012) A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity. Plos Pathogens 8(8):e1002854

2011

• Ortori, C.A., Dubern, J-F, Chhabra, S.R, Cámara, M., Hardie, K.R., Williams, P.and Barrett, D.A.. (2011). Simultaneous quantitative profiling of N-acylhomserine lactone and 2-alkyl -4(1H)-quinolone families of quorum sensing signaling molecules using LC-MS/MS. Analytical and Bioanalytical Chemistry 399(2):839-850.

2010

·         O’Toole, PW., Snelling, W.J., Canchaya, C., Forde, B.M., Hardie, K.R., Josenhans, C., Graham, R.L.J., McMullan, G., Parkhill, J., Belda, E., and Bentley, S.D. (2010) Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen. BMC Genomics 11:164.

·         Doherty, N., Shen, F-F., Halliday, N., Barrett, D., Hardie, KR., Winzer, K., and Atherton, J.C. (2010) In Helicobacter pylori, LuxS is a key enzyme in cysteine provision through a reverse transulphuration pathway. J. Bacteriology 192.5:1184-1192

·         Halliday, N.M, Hardie, K.R., Williams, P., Winzer, K, and Barrett, D.A. (2010) Quantitiative LC-MSMS profiling of activated methyl pathway metabolites in Escerichia coli LuxS-dependent quorum sensing. Analytical Biochemistry 403(1-2)20-29.

·         Shen, F-F., Hobley, L., Doherty, N., Loh, J.T., Cover, T.L., Sockett, R.E., Hardie, K.R., and Atherton, J.C. (2010) In Helicobacter pylori auto-inducer-2, but not LuxS/MccAB catalysed reverse transulphuration, regulates motility through modulation of flagellar gene transcription. In press BMC Microbiology.

·         Hardie, K.R., and Verran, J., (2010) Come Dine with Microbes. Microbiology Today. 37:190-191.

2009

·         Hardie, K.R., Pommier, S., and Wilhelm, S. (2009) The secreted proteins of Pseudomonas aeruginosa: their export machineries, and how they contribute to pathogenesis. Bacterial secreted proteins and pathogenesis Horizon Bioscience ISBN: 978-1-904455-42-4

·         Heurlier, K., Vendeville, A., Halliday, N., Green, A., Winzer, K., Tang, C.M., and Hardie, K.R. (2009) Growth deficiencies of Neisseria meningitidis pfs and luxS mutants are not due to inactivation of quorum-sensing. J. Bacteriology 191.4: 1293-1302.

·         Holmes, K., Tavender, T.J, Winzer, K., Wells. J.M., and Hardie, K.R (2009) AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro. BMC Microbiology 9:214-225

2008

·         Hardie, K.R., and Heurlier, K. (2008) Communication in bacterial communities: contribution of LuxS to Biofilm development. Nature Reviews Microbiology 6(8):635-643.

·         Wand, M.E., Sockett, R.E., Evans, K.J., Sharp, P.M., Hardie, K.R., and Winzer, K. (2006) Helicobacter pylori FlhB function: The FlhB C-terminal homologue HP1575 acts as a ‘spare part’ to permit flagellar export when the HP0770 FlhBCC domain is deleted. J. Bacteriology 188:7531-7541.

·         Argent, R.H., Thomas, R.J, Letley, D.P., Rittig, M.G, Hardie, K.R., and Atherton, J.C. (2008) Functional association between the Helicobacter pylori virulence factors VacA and CagA. J. Medical Microbiology 57: 145-150.

·         Tavender, T.,J Halliday, N.M., Hardie, K.R and Winzer, K., (2008) LuxS-independent formation of AI-2 from ribulose-5-phosphate. BMC Microbiol. 8:98-106.

2006

·         Wand, M.E., Sockett, R.E., Evans, K.J., Sharp, P.M., Hardie, K.R., and Winzer, K. (2006) Helicobacter pylori FlhB function: The FlhB C-terminal homologue HP1575 acts as a ‘spare part’ to permit flagellar export when the HP0770 FlhBCC domain is deleted. J. Bacteriology 188:7531-7541.

2005

·         Vendeville, A., Winzer, K., Heurlier, K., Tang, C.M., and Hardie, K.R. (2005) Making ‘sense’ of metabolism: Autoinducer-2, LuxS and  pathogenic bacteria. Nature Reviews Microbiology 3, 383-396.

·         Hardie, K.R, Baldwin, T., and Williams, P. (2005) Molecular basis of bacterial adaptation to a pathogenic lifestyle. Topley and Wilson’s Microbiology and Microbial Infections: Bacteriology. 10th Edition. Hodder Arnold Volume 1 chapter 6: 146-183.

·         Fitchen N., Letley D., Atherton, J.C., O’Shea, P, Williams, P. and Hardie K.R. (2005) The secreted cytotoxin VacA reabsorbs to the bacterial cell surface in a non-vacuolating form. J. Medical Microbiology 54: 621-630

2003

·         Fitchen N., O’Shea, P, Williams, P. and Hardie K.R. (2003) Electrostatic sensor for identifying interactions between peptides and bacterial membranes. Molecular Immunology 40, 407-413

·         Winzer, K., Hardie, K.R., and Williams, P. (2003) LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria. Advances in Applied Microbiology, Academic Press, San Diego, USA. Volume 53: 291-396

·         Cooksley C., Jenks P., Green, AD., Cockayne A., Logan R., and Hardie, K.R. (2003) NapA protects H. pylori from oxidative stress damage, and is regulated by the ferric uptake regulator. J. Medical Microbiology. 52: 461-469

·         Hardie, K.R., Cooksley, C., Green, A.D., and Winzer, K. (2003) Autoinducer 2 activity in E.coli culture supernatants can be actively reduced despite maintenance of an active synthase, LuxS. Microbiology 149: 715-728.

·         Fitchen N., Williams P., and Hardie K.R. (2003). Functional complementation of E. coli secD and secG mutants by Helicobacter pylori homologues. FEMS Letts 229: 57-63.

2002

·         Winzer, K., Hardie, K.R., and  Williams, P.  (2002) Bacterial Cell-cell communication: Can't talk now: gone to lunch. Current Opinions in Microbiology. 5.2:216-222.

·         Burgess, N.A., Kirke, D.F., Williams, P., Winzer, K, Hardie, K.R, Meyers, N.L., Aduse-Opoku, J., Curtis, M.A., and Camara, M. (2002) LuxS-dependent quorum sensing in Porphyromonas gingivalis modulates protease and haemaggluntinin activities but is not essential for virulence. Microbiology: 148:763-772

·         Winzer, K., Hardie, K.R., Burgess, N., Doherty, N., Kirke, D., Holden, M.T.G., Linforth, R., Cornell, K.A., Taylor, A.J., Hill, P.J., and Williams, P. (2002) LuxS: its role in central metabolism and the in vitro synthesis of 4-Hydroxy-5-methyl-3(2H)-furanone. Microbiology 148:909-922.

·         Winzer, K., Sun, Y-H, Green, A., Delory, M., Blackley, D., Hardie, K.R., Baldwin, T.J., and Tang, C.M. (2002) The role of Neisseria meningitidis luxS in cell-to-cell signaling and bacteraemic infection. Infect. Immunol. 70.4:2245-2248.

·         Kaur, M., Jumel, K., Hardie, K.R., Hardman, A., Meadows, J., and Melia,C.D. (2002) Determining the molar mass of a plasma substitute succinylated gelatin by SEC-MALLS, sedimentation equilibrium and conventional SEC. J. Chromatography 957: 139-148.

Pre-2000

·         Guilvout, I., Hardie, K.R., Sauvonnet, N., and Pugsley, A.P. (1999) Dissection of the outer membrane secretin PulD: Are there specific domains for multimerization and secretion specificity? J. Bacteriology 181:7212-7220.

·         Filloux, A., and Hardie, K.R. A systematic approach to the study of protein secretion in Gram-negative bacteria. (1998) Methods in Microbiology:Bacterial Pathogenesis, Academic Press. Eds: Paul Williams, Peter Williams, George Salmond, Julian Ketley.

·         Jiang, Y., Camara, M., Chhabra, R., Hardie, K.R, Bycroft, B., Lazdunski, A., Salmond, G., Stewart, G., and Williams, P. (1998) In vitro biosynthesis of the Pseudomonas aeruginosa quorum sensing signal molecule, N-butanoyl-L-homoserine lactone. Molecular Microbiology 28:193-203.

·         Hardie, K.R. and Williams, P. Fractionation of bacterial cell envelopes. (1998) Methods in Microbiology: Bacterial Pathogenesis, Academic Press. Eds: Paul Williams, Peter Williams, George Salmond, Julian Ketley.

·         Pugsley, A.P., Francetic, O., Possot, O.M., Sauvonnet, N.  and Hardie, K.R.  (1997).Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria. Gene 192.1:13-19.

·         Daefler, S., Guilvout, I., Hardie, K.R., Pugsley, A.P., and Russel, M.. (1997) The C-terminal domain of the secretin PulD contains the binding site for its cognate chaperone, PulS, and confers PulS-dependence for pIVf1 function. Molecular Microbiology 24.3:465-475.

·         McGowan, S.J., Sebaihia, M., O’Leary, S., Hardie, K.R., Williams, P., Bycroft, B.W., Stewart, G.S.A.B., and Salmond, G.P.C. (1997) Analysis of the carbapenem gene cluster of Erwinia carotovora: definition of the antibiotic biosynthetic genes and evidence for a novel b-lactam resistance mechanism. Molecular Microbiology 26:545-556.

·         Pugsley, A.P., Francetic, O., Hardie, K.R, Possot, O.M., Sauvonnet, N., and Seydel, A. (1997) Pullulanase: Model protein substrate for the General Secretory Pathway of Gram-negative Bacteria. Folia Microbiol. 42:184-192.

·         Stanley, P., Koronakis, V., Hardie, K.R.,  and Hughes, C. (1996). Independent interaction of the acyltransferase HlyC with two maturation domains of the E. coli toxin HlyA. Molecular Microbiology 20: 813-822

·         Hardie, K.R., Lory, S.,  and Pugsley, A.P.  (1996). Chaperone-mediated insertion of an outer membrane protein in Escherichia coli. EMBO Journal 15.5:978-98

·         Hardie, K.R., Seydel, A., Guilvout, I.,  and Pugsley, A.P.  (1996) The secretin-specific, chaperone-like protein of the general secretory pathway: separation of proteolytic protection and piloting functions. Molecular Microbiology 22.5:967-977.

·         Hardie, K.R.,  Schulze, A., Parker, M.W., and Buckley, J.T. (1995). Vibrio sp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation. Molecular Microbiology 17.6:1035-1044

·         Hughes, C., Hardie, K.R., Koronakis, E., Bailey, M., Stanley, P.  and Koronakis, V. (1994). Synthesis, maturation and secretion of E. coli hemolysin and related toxins. Proceedings of the Sixth European Toxin Symposium: In Zeitallblatt fur Bakteriologie. Fisher Verlog. In Bacterial protein toxins p183.

·         Benz, R., Hardie, K.R.,  and Hughes, C. (1994) Pore-formation in artificial membranes by the secreted hemolysins of Proteus vulgaris and Morganella morganii. European Journal of Biochemistry 220:339-347.

·         Curran, R., Hardie, K.R., and Towner, K.J. (1994) Analysis of pulsed-field gel electrophoresis of insertion mutations in the transferrin-binding system of Haemophilus influenzae type b. J. Medical Microbiology 41:120-126.

·         van der Goot, F.G.,  Hardie, K.R., Parker, M.W., and Buckley, J.T.  (1994). The C-terminal activation peptide produced upon proteolytic activation of the cytolytic toxin aerolysin is not involved in channel formation. J. Biological Chemistry 269: 30496-30501

·         Hughes, C., Issartel, J-P., Hardie, K.R., Stanley, P.,  Koronakis, E.,  and Koronakis, V. (1992) Activation of E. coli prohemolysin to the membrane-targeted toxin by HlyC-directed ACP-dependent fatty acylation. FEMS Microbiology Immunology 105:37-44.

·         Hardie, K.R., Issartel, J-P., Koronakis, E., Hughes, C.,  and  Koronakis, V. (1991). In vitro  activation of Escherichia coli prohaemolysin to the mature membrane-targeted toxin requires HlyC and a low molecular-weight cytosolic polypeptide. Molecular Microbiology 5.7:1669-1681.