2021 Worm meeting workshop on RNA imaging

This workshop was held within the online 2021 International worm meeting organized by GSA.

Organizers: Christopher M. Hammell, Cold Spring Harbor Laboratory, Erin Nishimura, Colorado State University, Sevinc Ercan, New York University

A brief description: Imaging single molecules in intact cells has the potential to reveal features of gene expression that are not possible to measure using standard, ensemble-based strategies. While a number of model organisms have successfully employed aptamer-based transcript imaging systems (MS2, PP7, etc.) to track individual RNAs in real time, these approaches have had only limited success in C. elegans. This workshop intends to build momentum toward establishing these systems throughout C. elegans research community which will complement this powerful genetic model and enable aspects of RNA transcription, export, localization, translation, and turnover to be studied in detail.

Speakers and presentations:

ChangHwan Lee (SUNY Albany), “Capturing dynamics of transcriptional bursting in vivo using the MS2 system.”

Hongjie Zhang, Universidade de Macau, “PP7/PCP-based visualization of membrane-associated transcripts in epithelia.”

Wolfgang Keil, Curie Institute, “Monitoring spatiotemporal patterns of post-embryonic miRNA transcription using the MS2 system.”

Erin Osborne Nishimura, Colorado State University, “Best practices in mRNA live imaging.”

Note: Also check out work from Antoine Barriere from Aix Marseille University presented in the worm meeting and published at: https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab469/6295541