• Prepared coded slides of brain tissue samples, stained using Golgi-impregnation method
• Evaluated Golgi-impregnated neurons for in-depth morphological analysis (i.e. amount/complexity of dendritic branching, length of dendritic branches, dendritic spine density and spine configurations, soma size, etc.)
• Generated camera lucida drawings of randomly selected neurons from given population
• Performed sholl and branch point analyses to convert drawings into quantitative data for statistical comparisons
• Interpreted and integrated aforementioned data to study effects of various diseases and treatment strategies on the health and integrity of the neurons and their associated circuitry
August 2012-December 2012
Research Volunteer, USF Health Byrd Alzheimer’s Institute
Molecular Pharmacology & Physiology, Neurobiology of Learning & Memory Laboratory
PI: Dr. Edwin Weeber, Professor/Director of Lab; Supervisors: Justin Trotter, Graduate Student and Dr. April Lussier, Post-Doctoral Fellow
• Collected tissue samples of full bird brain specimens using a microtome
• Mounted thin slices of brain tissue on slides and fluorescently stained samples
• Coded behavior for mice performing Forced Swimming Test (FST) to evaluate activity of antidepressant
• Responsible for other duties as assigned
June & July 2012
Summer Research Assistant, University of South Florida—Tampa, FL
Chemical & Biomedical Engineering, “Photodegradation Rate Measurements for Low Cost SelfCleaning Cement Surfaces”
Research Advisor: Dr. Vinay K. Gupta, Professor, Department of Chemical & Biomedical Engineering
• Performed qualitative and quantitative experiments for measuring photodegradation of an organic dye, methyl orange, in titania and alumina samples
• Generated silver-silica core-shell nanoparticles using the Stöber method
• Recorded effect of addition of Ag-Si nanoparticles to samples and their photocatalytic activity
June & July 2011
Summer Research Assistant, University of South Florida—Tampa, FL
Chemical & Biomedical Engineering, Global Center for Hearing and Speech Research
“The Aging Auditory System: Presbycusis and Its Neural Basis”
Research Advisor: Dr. Robert Frisina, Jr., Professor, Department of Chemical & Biomedical Engineering; Supervisors: Xiaoxia Zhu and Bo Ding
• Investigated potential mechanisms of hearing loss on a neural basis (i.e. how aldosterone may help reverse hearing loss by restoring cochlear function)
• Collected and compiled relevant literature on gamma-aminobutyric acid (GABA), its overall trends with age and the effects of GABAergic drugs on hearing in the aged, specifically how administration of an antiepileptic drug might increase overall GABA concentration and thereby improve temporal processing abilities
• Calibrated and operated computer-aided system (TDT System 3 instrumentation with BioSigRP/SigGenRP software) for collecting auditory data
• Interpreted results of ABR (auditory-evoked brainstem response) thresholds and patterns in young and aged mice